Novel endoscopes for microscopic assessment of airway clearance using micro-optical coherence tomography
Thesis: S.M., Harvard-MIT Program in Health Sciences and Technology, 2015.
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| Other Authors: | |
| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2015
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| Online Access: | http://hdl.handle.net/1721.1/97830 |
| _version_ | 1826204292122411008 |
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| author | Unglert, Carolin Isabella |
| author2 | Guillermo J. Tearney. |
| author_facet | Guillermo J. Tearney. Unglert, Carolin Isabella |
| author_sort | Unglert, Carolin Isabella |
| collection | MIT |
| description | Thesis: S.M., Harvard-MIT Program in Health Sciences and Technology, 2015. |
| first_indexed | 2024-09-23T12:51:58Z |
| format | Thesis |
| id | mit-1721.1/97830 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T12:51:58Z |
| publishDate | 2015 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/978302019-04-12T15:56:09Z Novel endoscopes for microscopic assessment of airway clearance using micro-optical coherence tomography Unglert, Carolin Isabella Guillermo J. Tearney. Harvard--MIT Program in Health Sciences and Technology. Harvard--MIT Program in Health Sciences and Technology. Harvard--MIT Program in Health Sciences and Technology. Thesis: S.M., Harvard-MIT Program in Health Sciences and Technology, 2015. Cataloged from PDF version of thesis. Includes bibliographical references (pages 73-77). The health of the human respiratory system depends critically on airway clearance via motile hair-like structures (cilia), which transport and eliminate unwanted particles trapped within mucus. Impairment of mucociliary clearance (MCC) can lead to life-threatening airway narrowing and lung infections, and is a major cause of morbidity and mortality in patients with cystic fibrosis, primary ciliary dyskinesia and chronic obstructive lung disease. However, no tool for microscopic in-vivo visualization of ciliary function is currently available, limiting studies of disease pathogenesis, refined diagnosis and phenotyping, and the development of novel therapeutics. In this thesis, a novel, 1-pm resolution, optical interferometric imaging technique termed Micro-OCT was incorporated into miniaturized common-path endoscopes and mucociliary transport was visualized in vivo for the first time. The first-generation Micro-OCT probe had a rigid design with outer diameter of 4 mm and a two-prism configuration providing beam splitting and sample beam shaping into an annular profile. Image quality of the probe allowed visualization of the periodic pattern of ciliary beating, measurement of airway surface liquid depth (ASL) and visualization of mucociliary transport. Unaltered ciliary function was demonstrated in a living, spontaneously breathing swine model. Newer generation common-path endoscope designs were demonstrated that improve, among other limitations, the stability of the reference reflector position and provide greater potential for miniaturization. The presented work opens unprecedented avenues for studying MCC and the effect of novel therapeutics within the complexity of a living organism. Further, it lays the groundwork for the development of a human probe with the potential to revolutionize diagnosis, phenotyping, and therapy management for all patients with respiratory disease involving the mucociliary escalator. by Carolin Isabella Unglert. S.M. 2015-07-17T19:51:03Z 2015-07-17T19:51:03Z 2015 2015 Thesis http://hdl.handle.net/1721.1/97830 913232611 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 77 pages application/pdf Massachusetts Institute of Technology |
| spellingShingle | Harvard--MIT Program in Health Sciences and Technology. Unglert, Carolin Isabella Novel endoscopes for microscopic assessment of airway clearance using micro-optical coherence tomography |
| title | Novel endoscopes for microscopic assessment of airway clearance using micro-optical coherence tomography |
| title_full | Novel endoscopes for microscopic assessment of airway clearance using micro-optical coherence tomography |
| title_fullStr | Novel endoscopes for microscopic assessment of airway clearance using micro-optical coherence tomography |
| title_full_unstemmed | Novel endoscopes for microscopic assessment of airway clearance using micro-optical coherence tomography |
| title_short | Novel endoscopes for microscopic assessment of airway clearance using micro-optical coherence tomography |
| title_sort | novel endoscopes for microscopic assessment of airway clearance using micro optical coherence tomography |
| topic | Harvard--MIT Program in Health Sciences and Technology. |
| url | http://hdl.handle.net/1721.1/97830 |
| work_keys_str_mv | AT unglertcarolinisabella novelendoscopesformicroscopicassessmentofairwayclearanceusingmicroopticalcoherencetomography |