Inhibiting the Activity of CA1 Hippocampal Neurons Prevents the Recall of Contextual Fear Memory in Inducible ArchT Transgenic Mice
The optogenetic manipulation of light-activated ion-channels/pumps (i.e., opsins) can reversibly activate or suppress neuronal activity with precise temporal control. Therefore, optogenetic techniques hold great potential to establish causal relationships between specific neuronal circuits and their...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2015
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| Online Access: | http://hdl.handle.net/1721.1/98168 https://orcid.org/0000-0002-0419-3351 |
| _version_ | 1826204990504435712 |
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| author | Sakaguchi, Masanori Kim, Karam Yu, Lily Mae Yee Hashikawa, Yoshiko Sekine, Yukiko Okumura, Yuki Kawano, Masako Hayashi, Masanobu Kumar, Deependra McHugh, Thomas J. Hayashi, Yasunori Boyden, Edward |
| author2 | Massachusetts Institute of Technology. Media Laboratory |
| author_facet | Massachusetts Institute of Technology. Media Laboratory Sakaguchi, Masanori Kim, Karam Yu, Lily Mae Yee Hashikawa, Yoshiko Sekine, Yukiko Okumura, Yuki Kawano, Masako Hayashi, Masanobu Kumar, Deependra McHugh, Thomas J. Hayashi, Yasunori Boyden, Edward |
| author_sort | Sakaguchi, Masanori |
| collection | MIT |
| description | The optogenetic manipulation of light-activated ion-channels/pumps (i.e., opsins) can reversibly activate or suppress neuronal activity with precise temporal control. Therefore, optogenetic techniques hold great potential to establish causal relationships between specific neuronal circuits and their function in freely moving animals. Due to the critical role of the hippocampal CA1 region in memory function, we explored the possibility of targeting an inhibitory opsin, ArchT, to CA1 pyramidal neurons in mice. We established a transgenic mouse line in which tetracycline trans-activator induces ArchT expression. By crossing this line with a CaMKIIα-tTA transgenic line, the delivery of light via an implanted optrode inhibits the activity of excitatory CA1 neurons. We found that light delivery to the hippocampus inhibited the recall of a contextual fear memory. Our results demonstrate that this optogenetic mouse line can be used to investigate the neuronal circuits underlying behavior. |
| first_indexed | 2024-09-23T13:05:20Z |
| format | Article |
| id | mit-1721.1/98168 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:05:20Z |
| publishDate | 2015 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/981682022-09-28T11:52:55Z Inhibiting the Activity of CA1 Hippocampal Neurons Prevents the Recall of Contextual Fear Memory in Inducible ArchT Transgenic Mice Sakaguchi, Masanori Kim, Karam Yu, Lily Mae Yee Hashikawa, Yoshiko Sekine, Yukiko Okumura, Yuki Kawano, Masako Hayashi, Masanobu Kumar, Deependra McHugh, Thomas J. Hayashi, Yasunori Boyden, Edward Massachusetts Institute of Technology. Media Laboratory Program in Media Arts and Sciences (Massachusetts Institute of Technology) Boyden, Edward Stuart The optogenetic manipulation of light-activated ion-channels/pumps (i.e., opsins) can reversibly activate or suppress neuronal activity with precise temporal control. Therefore, optogenetic techniques hold great potential to establish causal relationships between specific neuronal circuits and their function in freely moving animals. Due to the critical role of the hippocampal CA1 region in memory function, we explored the possibility of targeting an inhibitory opsin, ArchT, to CA1 pyramidal neurons in mice. We established a transgenic mouse line in which tetracycline trans-activator induces ArchT expression. By crossing this line with a CaMKIIα-tTA transgenic line, the delivery of light via an implanted optrode inhibits the activity of excitatory CA1 neurons. We found that light delivery to the hippocampus inhibited the recall of a contextual fear memory. Our results demonstrate that this optogenetic mouse line can be used to investigate the neuronal circuits underlying behavior. 2015-08-20T18:55:23Z 2015-08-20T18:55:23Z 2015-06 2015-03 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/98168 Sakaguchi, Masanori, Karam Kim, Lily Mae Yee Yu, Yoshiko Hashikawa, Yukiko Sekine, Yuki Okumura, Masako Kawano, et al. “Inhibiting the Activity of CA1 Hippocampal Neurons Prevents the Recall of Contextual Fear Memory in Inducible ArchT Transgenic Mice.” Edited by Masuo Ohno. PLoS ONE 10, no. 6 (June 15, 2015): e0130163. https://orcid.org/0000-0002-0419-3351 en_US http://dx.doi.org/10.1371/journal.pone.0130163 PLOS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Public Library of Science Public Library of Science |
| spellingShingle | Sakaguchi, Masanori Kim, Karam Yu, Lily Mae Yee Hashikawa, Yoshiko Sekine, Yukiko Okumura, Yuki Kawano, Masako Hayashi, Masanobu Kumar, Deependra McHugh, Thomas J. Hayashi, Yasunori Boyden, Edward Inhibiting the Activity of CA1 Hippocampal Neurons Prevents the Recall of Contextual Fear Memory in Inducible ArchT Transgenic Mice |
| title | Inhibiting the Activity of CA1 Hippocampal Neurons Prevents the Recall of Contextual Fear Memory in Inducible ArchT Transgenic Mice |
| title_full | Inhibiting the Activity of CA1 Hippocampal Neurons Prevents the Recall of Contextual Fear Memory in Inducible ArchT Transgenic Mice |
| title_fullStr | Inhibiting the Activity of CA1 Hippocampal Neurons Prevents the Recall of Contextual Fear Memory in Inducible ArchT Transgenic Mice |
| title_full_unstemmed | Inhibiting the Activity of CA1 Hippocampal Neurons Prevents the Recall of Contextual Fear Memory in Inducible ArchT Transgenic Mice |
| title_short | Inhibiting the Activity of CA1 Hippocampal Neurons Prevents the Recall of Contextual Fear Memory in Inducible ArchT Transgenic Mice |
| title_sort | inhibiting the activity of ca1 hippocampal neurons prevents the recall of contextual fear memory in inducible archt transgenic mice |
| url | http://hdl.handle.net/1721.1/98168 https://orcid.org/0000-0002-0419-3351 |
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