Quantum dot/antibody conjugates for in vivo cytometric imaging in mice
Multiplexed, phenotypic, intravital cytometric imaging requires novel fluorophore conjugates that have an appropriate size for long circulation and diffusion and show virtually no nonspecific binding to cells/serum while binding to cells of interest with high specificity. In addition, these conjugat...
| Main Authors: | , , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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National Academy of Sciences (U.S.)
2015
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| Online Access: | http://hdl.handle.net/1721.1/98406 https://orcid.org/0000-0003-2220-4365 |
| _version_ | 1811077993874849792 |
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| author | Han, Hee-Sun Niemeyer, Elisabeth Huang, Yuhui Kamoun, Walid S. Martin, John Daniel Bhaumik, Jayeeta Chen, Yunching Roberge, Sylvie Cui, Jian Martin, Margaret R. Fukumura, Dai Jain, Rakesh K. Bawendi, Moungi G. Duda, Dan G. |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Han, Hee-Sun Niemeyer, Elisabeth Huang, Yuhui Kamoun, Walid S. Martin, John Daniel Bhaumik, Jayeeta Chen, Yunching Roberge, Sylvie Cui, Jian Martin, Margaret R. Fukumura, Dai Jain, Rakesh K. Bawendi, Moungi G. Duda, Dan G. |
| author_sort | Han, Hee-Sun |
| collection | MIT |
| description | Multiplexed, phenotypic, intravital cytometric imaging requires novel fluorophore conjugates that have an appropriate size for long circulation and diffusion and show virtually no nonspecific binding to cells/serum while binding to cells of interest with high specificity. In addition, these conjugates must be stable and maintain a high quantum yield in the in vivo environments. Here, we show that this can be achieved using compact (~15 nm in hydrodynamic diameter) and biocompatible quantum dot (QD) -Ab conjugates. We developed these conjugates by coupling whole mAbs to QDs coated with norbornene-displaying polyimidazole ligands using tetrazine–norbornene cycloaddition. Our QD immunoconstructs were used for in vivo single-cell labeling in bone marrow. The intravital imaging studies using a chronic calvarial bone window showed that our QD-Ab conjugates diffuse into the entire bone marrow and efficiently label single cells belonging to rare populations of hematopoietic stem and progenitor cells (Sca1[superscript +]c-Kit[superscript +] cells). This in vivo cytometric technique may be useful in a wide range of structural and functional imaging to study the interactions between cells and between a cell and its environment in intact and diseased tissues. |
| first_indexed | 2024-09-23T10:51:42Z |
| format | Article |
| id | mit-1721.1/98406 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:51:42Z |
| publishDate | 2015 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/984062022-09-30T23:31:13Z Quantum dot/antibody conjugates for in vivo cytometric imaging in mice Han, Hee-Sun Niemeyer, Elisabeth Huang, Yuhui Kamoun, Walid S. Martin, John Daniel Bhaumik, Jayeeta Chen, Yunching Roberge, Sylvie Cui, Jian Martin, Margaret R. Fukumura, Dai Jain, Rakesh K. Bawendi, Moungi G. Duda, Dan G. Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Chemistry Han, Hee-Sun Martin, John Daniel Cui, Jian Bawendi, Moungi G. Multiplexed, phenotypic, intravital cytometric imaging requires novel fluorophore conjugates that have an appropriate size for long circulation and diffusion and show virtually no nonspecific binding to cells/serum while binding to cells of interest with high specificity. In addition, these conjugates must be stable and maintain a high quantum yield in the in vivo environments. Here, we show that this can be achieved using compact (~15 nm in hydrodynamic diameter) and biocompatible quantum dot (QD) -Ab conjugates. We developed these conjugates by coupling whole mAbs to QDs coated with norbornene-displaying polyimidazole ligands using tetrazine–norbornene cycloaddition. Our QD immunoconstructs were used for in vivo single-cell labeling in bone marrow. The intravital imaging studies using a chronic calvarial bone window showed that our QD-Ab conjugates diffuse into the entire bone marrow and efficiently label single cells belonging to rare populations of hematopoietic stem and progenitor cells (Sca1[superscript +]c-Kit[superscript +] cells). This in vivo cytometric technique may be useful in a wide range of structural and functional imaging to study the interactions between cells and between a cell and its environment in intact and diseased tissues. National Institutes of Health (U.S.) (Grant U54-CA151884) National Institutes of Health (U.S.) (Grant P41-EB015871-26A1) Samsung Scholarship Foundation (Graduate Student Fellowship) Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies (Grant W911NF-07-D-0004) 2015-09-08T18:08:06Z 2015-09-08T18:08:06Z 2015-02 2014-06 Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/98406 Han, Hee-Sun, Elisabeth Niemeyer, Yuhui Huang, Walid S. Kamoun, John D. Martin, Jayeeta Bhaumik, Yunching Chen, et al. “Quantum Dot/antibody Conjugates for in Vivo Cytometric Imaging in Mice.” Proc Natl Acad Sci USA 112, no. 5 (January 20, 2015): 1350–1355. https://orcid.org/0000-0003-2220-4365 en_US http://dx.doi.org/10.1073/pnas.1421632111 Proceedings of the National Academy of Sciences Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) National Academy of Sciences (U.S.) |
| spellingShingle | Han, Hee-Sun Niemeyer, Elisabeth Huang, Yuhui Kamoun, Walid S. Martin, John Daniel Bhaumik, Jayeeta Chen, Yunching Roberge, Sylvie Cui, Jian Martin, Margaret R. Fukumura, Dai Jain, Rakesh K. Bawendi, Moungi G. Duda, Dan G. Quantum dot/antibody conjugates for in vivo cytometric imaging in mice |
| title | Quantum dot/antibody conjugates for in vivo cytometric imaging in mice |
| title_full | Quantum dot/antibody conjugates for in vivo cytometric imaging in mice |
| title_fullStr | Quantum dot/antibody conjugates for in vivo cytometric imaging in mice |
| title_full_unstemmed | Quantum dot/antibody conjugates for in vivo cytometric imaging in mice |
| title_short | Quantum dot/antibody conjugates for in vivo cytometric imaging in mice |
| title_sort | quantum dot antibody conjugates for in vivo cytometric imaging in mice |
| url | http://hdl.handle.net/1721.1/98406 https://orcid.org/0000-0003-2220-4365 |
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