Optogenetic control of nerve growth
Due to the limited regenerative ability of neural tissue, a diverse set of biochemical and biophysical cues for increasing nerve growth has been investigated, including neurotrophic factors, topography, and electrical stimulation. In this report, we explore optogenetic control of neurite growth as a...
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2015
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| Online Access: | http://hdl.handle.net/1721.1/98436 https://orcid.org/0000-0001-6495-5197 https://orcid.org/0000-0002-8525-8451 https://orcid.org/0000-0002-8669-0246 https://orcid.org/0000-0002-3376-6358 https://orcid.org/0000-0003-4890-6103 |
| _version_ | 1826194250954440704 |
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| author | Park, Seongjun Koppes, Ryan Jia, Xiaoting Achyuta, Anil Kumar H. McLaughlin, Bryan L. Froriep, Ulrich Paul Anikeeva, Polina Olegovna |
| author2 | Massachusetts Institute of Technology. Department of Materials Science and Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Materials Science and Engineering Park, Seongjun Koppes, Ryan Jia, Xiaoting Achyuta, Anil Kumar H. McLaughlin, Bryan L. Froriep, Ulrich Paul Anikeeva, Polina Olegovna |
| author_sort | Park, Seongjun |
| collection | MIT |
| description | Due to the limited regenerative ability of neural tissue, a diverse set of biochemical and biophysical cues for increasing nerve growth has been investigated, including neurotrophic factors, topography, and electrical stimulation. In this report, we explore optogenetic control of neurite growth as a cell-specific alternative to electrical stimulation. By investigating a broad range of optical stimulation parameters on dorsal root ganglia (DRGs) expressing channelrhodopsin 2 (ChR2), we identified conditions that enhance neurite outgrowth by three-fold as compared to unstimulated or wild-type (WT) controls. Furthermore, optogenetic stimulation of ChR2 expressing DRGs induces directional outgrowth in WT DRGs co-cultured within a 10 mm vicinity of the optically sensitive ganglia. This observed enhancement and polarization of neurite growth was accompanied by an increased expression of neural growth and brain derived neurotrophic factors (NGF, BDNF). This work highlights the potential for implementing optogenetics to drive nerve growth in specific cell populations. |
| first_indexed | 2024-09-23T09:53:12Z |
| format | Article |
| id | mit-1721.1/98436 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:53:12Z |
| publishDate | 2015 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/984362022-09-26T14:19:30Z Optogenetic control of nerve growth Park, Seongjun Koppes, Ryan Jia, Xiaoting Achyuta, Anil Kumar H. McLaughlin, Bryan L. Froriep, Ulrich Paul Anikeeva, Polina Olegovna Massachusetts Institute of Technology. Department of Materials Science and Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Research Laboratory of Electronics Simons Center for the Social Brain (Massachusetts Institute of Technology) Park, Seongjun Koppes, Ryan Froriep, Ulrich Paul Jia, Xiaoting Anikeeva, Polina Olegovna Due to the limited regenerative ability of neural tissue, a diverse set of biochemical and biophysical cues for increasing nerve growth has been investigated, including neurotrophic factors, topography, and electrical stimulation. In this report, we explore optogenetic control of neurite growth as a cell-specific alternative to electrical stimulation. By investigating a broad range of optical stimulation parameters on dorsal root ganglia (DRGs) expressing channelrhodopsin 2 (ChR2), we identified conditions that enhance neurite outgrowth by three-fold as compared to unstimulated or wild-type (WT) controls. Furthermore, optogenetic stimulation of ChR2 expressing DRGs induces directional outgrowth in WT DRGs co-cultured within a 10 mm vicinity of the optically sensitive ganglia. This observed enhancement and polarization of neurite growth was accompanied by an increased expression of neural growth and brain derived neurotrophic factors (NGF, BDNF). This work highlights the potential for implementing optogenetics to drive nerve growth in specific cell populations. Charles Stark Draper Laboratory (University Research and Development Grant) National Science Foundation (U.S.). Materials Research Science and Engineering Centers (Program) (Award DMR-0819762) National Science Foundation (U.S.) (CAREER Award CBET-1253890) Simons Foundation Korean Government Scholarship Program for Study Overseas 2015-09-10T16:03:41Z 2015-09-10T16:03:41Z 2015-05 2014-10 Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/98436 Park, Seongjun, Ryan A. Koppes, Ulrich P. Froriep, Xiaoting Jia, Anil Kumar H. Achyuta, Bryan L. McLaughlin, and Polina Anikeeva. “Optogenetic Control of Nerve Growth.” Scientific Reports 5 (May 18, 2015): 9669. https://orcid.org/0000-0001-6495-5197 https://orcid.org/0000-0002-8525-8451 https://orcid.org/0000-0002-8669-0246 https://orcid.org/0000-0002-3376-6358 https://orcid.org/0000-0003-4890-6103 en_US http://dx.doi.org/10.1038/srep09669 Scientific Reports Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Nature Publishing Group |
| spellingShingle | Park, Seongjun Koppes, Ryan Jia, Xiaoting Achyuta, Anil Kumar H. McLaughlin, Bryan L. Froriep, Ulrich Paul Anikeeva, Polina Olegovna Optogenetic control of nerve growth |
| title | Optogenetic control of nerve growth |
| title_full | Optogenetic control of nerve growth |
| title_fullStr | Optogenetic control of nerve growth |
| title_full_unstemmed | Optogenetic control of nerve growth |
| title_short | Optogenetic control of nerve growth |
| title_sort | optogenetic control of nerve growth |
| url | http://hdl.handle.net/1721.1/98436 https://orcid.org/0000-0001-6495-5197 https://orcid.org/0000-0002-8525-8451 https://orcid.org/0000-0002-8669-0246 https://orcid.org/0000-0002-3376-6358 https://orcid.org/0000-0003-4890-6103 |
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