Targeted disruption of a single sex pheromone receptor gene completely abolishes in vivo pheromone response in the silkmoth
Male moths use species-specific sex pheromones to identify and orientate toward conspecific females. Odorant receptors (ORs) for sex pheromone substances have been identified as sex pheromone receptors in various moth species. However, direct in vivo evidence linking the functional role of these ORs...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Nature Publishing Group
2015
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| Online Access: | http://hdl.handle.net/1721.1/98441 https://orcid.org/0000-0003-2782-2509 |
| _version_ | 1811073915158528000 |
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| author | Sakurai, Takeshi Mitsuno, Hidefumi Mikami, Akihisa Uchino, Keiro Tabuchi, Masashi Zhang, Feng Sezutsu, Hideki Kanzaki, Ryohei |
| author2 | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Sakurai, Takeshi Mitsuno, Hidefumi Mikami, Akihisa Uchino, Keiro Tabuchi, Masashi Zhang, Feng Sezutsu, Hideki Kanzaki, Ryohei |
| author_sort | Sakurai, Takeshi |
| collection | MIT |
| description | Male moths use species-specific sex pheromones to identify and orientate toward conspecific females. Odorant receptors (ORs) for sex pheromone substances have been identified as sex pheromone receptors in various moth species. However, direct in vivo evidence linking the functional role of these ORs with behavioural responses is lacking. In the silkmoth, Bombyx mori, female moths emit two sex pheromone components, bombykol and bombykal, but only bombykol elicits sexual behaviour in male moths. A sex pheromone receptor BmOR1 is specifically tuned to bombykol and is expressed in specialized olfactory receptor neurons (ORNs) in the pheromone sensitive long sensilla trichodea of male silkmoth antennae. Here, we show that disruption of the BmOR1 gene, mediated by transcription activator-like effector nucleases (TALENs), completely removes ORN sensitivity to bombykol and corresponding pheromone-source searching behaviour in male moths. Furthermore, transgenic rescue of BmOR1 restored normal behavioural responses to bombykol. Our results demonstrate that BmOR1 is required for the physiological and behavioural response to bombykol, demonstrating that it is the receptor that mediates sex pheromone responses in male silkmoths. This study provides the first direct evidence that a member of the sex pheromone receptor family in moth species mediates conspecific sex pheromone information for sexual behaviour. |
| first_indexed | 2024-09-23T09:40:10Z |
| format | Article |
| id | mit-1721.1/98441 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:40:10Z |
| publishDate | 2015 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | mit-1721.1/984412022-09-30T16:03:46Z Targeted disruption of a single sex pheromone receptor gene completely abolishes in vivo pheromone response in the silkmoth Sakurai, Takeshi Mitsuno, Hidefumi Mikami, Akihisa Uchino, Keiro Tabuchi, Masashi Zhang, Feng Sezutsu, Hideki Kanzaki, Ryohei Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences McGovern Institute for Brain Research at MIT Zhang, Feng Male moths use species-specific sex pheromones to identify and orientate toward conspecific females. Odorant receptors (ORs) for sex pheromone substances have been identified as sex pheromone receptors in various moth species. However, direct in vivo evidence linking the functional role of these ORs with behavioural responses is lacking. In the silkmoth, Bombyx mori, female moths emit two sex pheromone components, bombykol and bombykal, but only bombykol elicits sexual behaviour in male moths. A sex pheromone receptor BmOR1 is specifically tuned to bombykol and is expressed in specialized olfactory receptor neurons (ORNs) in the pheromone sensitive long sensilla trichodea of male silkmoth antennae. Here, we show that disruption of the BmOR1 gene, mediated by transcription activator-like effector nucleases (TALENs), completely removes ORN sensitivity to bombykol and corresponding pheromone-source searching behaviour in male moths. Furthermore, transgenic rescue of BmOR1 restored normal behavioural responses to bombykol. Our results demonstrate that BmOR1 is required for the physiological and behavioural response to bombykol, demonstrating that it is the receptor that mediates sex pheromone responses in male silkmoths. This study provides the first direct evidence that a member of the sex pheromone receptor family in moth species mediates conspecific sex pheromone information for sexual behaviour. 2015-09-10T16:35:48Z 2015-09-10T16:35:48Z 2015-06 2015-02 Article http://purl.org/eprint/type/JournalArticle 2045-2322 http://hdl.handle.net/1721.1/98441 Sakurai, Takeshi, Hidefumi Mitsuno, Akihisa Mikami, Keiro Uchino, Masashi Tabuchi, Feng Zhang, Hideki Sezutsu, and Ryohei Kanzaki. “Targeted Disruption of a Single Sex Pheromone Receptor Gene Completely Abolishes in Vivo Pheromone Response in the Silkmoth.” Scientific Reports 5 (June 5, 2015): 11001. https://orcid.org/0000-0003-2782-2509 en_US http://dx.doi.org/10.1038/srep11001 Scientific Reports Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Nature Publishing Group Nature Publishing Group |
| spellingShingle | Sakurai, Takeshi Mitsuno, Hidefumi Mikami, Akihisa Uchino, Keiro Tabuchi, Masashi Zhang, Feng Sezutsu, Hideki Kanzaki, Ryohei Targeted disruption of a single sex pheromone receptor gene completely abolishes in vivo pheromone response in the silkmoth |
| title | Targeted disruption of a single sex pheromone receptor gene completely abolishes in vivo pheromone response in the silkmoth |
| title_full | Targeted disruption of a single sex pheromone receptor gene completely abolishes in vivo pheromone response in the silkmoth |
| title_fullStr | Targeted disruption of a single sex pheromone receptor gene completely abolishes in vivo pheromone response in the silkmoth |
| title_full_unstemmed | Targeted disruption of a single sex pheromone receptor gene completely abolishes in vivo pheromone response in the silkmoth |
| title_short | Targeted disruption of a single sex pheromone receptor gene completely abolishes in vivo pheromone response in the silkmoth |
| title_sort | targeted disruption of a single sex pheromone receptor gene completely abolishes in vivo pheromone response in the silkmoth |
| url | http://hdl.handle.net/1721.1/98441 https://orcid.org/0000-0003-2782-2509 |
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