Gas Transfer in Cellularized Collagen-Membrane Gas Exchange Devices
Chronic lower respiratory disease is highly prevalent in the United States, and there remains a need for alternatives to lung transplant for patients who progress to end-stage lung disease. Portable or implantable gas oxygenators based on microfluidic technologies can address this need, provided the...
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| Format: | Article |
| Language: | en_US |
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Mary Ann Liebert, Inc.
2015
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| Online Access: | http://hdl.handle.net/1721.1/98486 https://orcid.org/0000-0001-5981-2589 |
| _version_ | 1826200301664731136 |
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| author | Lo, Justin H. Bassett, Erik K. Penson, Elliot J. N. Hoganson, David M. Vacanti, Joseph P. |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Lo, Justin H. Bassett, Erik K. Penson, Elliot J. N. Hoganson, David M. Vacanti, Joseph P. |
| author_sort | Lo, Justin H. |
| collection | MIT |
| description | Chronic lower respiratory disease is highly prevalent in the United States, and there remains a need for alternatives to lung transplant for patients who progress to end-stage lung disease. Portable or implantable gas oxygenators based on microfluidic technologies can address this need, provided they operate both efficiently and biocompatibly. Incorporating biomimetic materials into such devices can help replicate native gas exchange function and additionally support cellular components. In this work, we have developed microfluidic devices that enable blood gas exchange across ultra-thin collagen membranes (as thin as 2 μm). Endothelial, stromal, and parenchymal cells readily adhere to these membranes, and long-term culture with cellular components results in remodeling, reflected by reduced membrane thickness. Functionally, acellular collagen-membrane lung devices can mediate effective gas exchange up to ~288 mL/min/m[superscript 2] of oxygen and ~685 mL/min/m[superscript 2] of carbon dioxide, approaching the gas exchange efficiency noted in the native lung. Testing several configurations of lung devices to explore various physical parameters of the device design, we concluded that thinner membranes and longer gas exchange distances result in improved hemoglobin saturation and increases in pO[subscript 2]. However, in the design space tested, these effects are relatively small compared to the improvement in overall oxygen and carbon dioxide transfer by increasing the blood flow rate. Finally, devices cultured with endothelial and parenchymal cells achieved similar gas exchange rates compared with acellular devices. Biomimetic blood oxygenator design opens the possibility of creating portable or implantable microfluidic devices that achieve efficient gas transfer while also maintaining physiologic conditions. |
| first_indexed | 2024-09-23T11:34:23Z |
| format | Article |
| id | mit-1721.1/98486 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:34:23Z |
| publishDate | 2015 |
| publisher | Mary Ann Liebert, Inc. |
| record_format | dspace |
| spelling | mit-1721.1/984862022-09-27T20:25:40Z Gas Transfer in Cellularized Collagen-Membrane Gas Exchange Devices Lo, Justin H. Bassett, Erik K. Penson, Elliot J. N. Hoganson, David M. Vacanti, Joseph P. Harvard University--MIT Division of Health Sciences and Technology Lo, Justin H. Chronic lower respiratory disease is highly prevalent in the United States, and there remains a need for alternatives to lung transplant for patients who progress to end-stage lung disease. Portable or implantable gas oxygenators based on microfluidic technologies can address this need, provided they operate both efficiently and biocompatibly. Incorporating biomimetic materials into such devices can help replicate native gas exchange function and additionally support cellular components. In this work, we have developed microfluidic devices that enable blood gas exchange across ultra-thin collagen membranes (as thin as 2 μm). Endothelial, stromal, and parenchymal cells readily adhere to these membranes, and long-term culture with cellular components results in remodeling, reflected by reduced membrane thickness. Functionally, acellular collagen-membrane lung devices can mediate effective gas exchange up to ~288 mL/min/m[superscript 2] of oxygen and ~685 mL/min/m[superscript 2] of carbon dioxide, approaching the gas exchange efficiency noted in the native lung. Testing several configurations of lung devices to explore various physical parameters of the device design, we concluded that thinner membranes and longer gas exchange distances result in improved hemoglobin saturation and increases in pO[subscript 2]. However, in the design space tested, these effects are relatively small compared to the improvement in overall oxygen and carbon dioxide transfer by increasing the blood flow rate. Finally, devices cultured with endothelial and parenchymal cells achieved similar gas exchange rates compared with acellular devices. Biomimetic blood oxygenator design opens the possibility of creating portable or implantable microfluidic devices that achieve efficient gas transfer while also maintaining physiologic conditions. National Institute of General Medical Sciences (U.S.) (MSTP T32GM007753) 2015-09-14T18:34:12Z 2015-09-14T18:34:12Z 2015-07 2014-06 Article http://purl.org/eprint/type/JournalArticle 1937-3341 1937-335X http://hdl.handle.net/1721.1/98486 Lo, Justin H., Erik K. Bassett, Elliot J. N. Penson, David M. Hoganson, and Joseph P. Vacanti. “Gas Transfer in Cellularized Collagen-Membrane Gas Exchange Devices.” Tissue Engineering Part A 21, no. 15–16 (August 2015): 2147–2155. © 2015 Mary Ann Liebert, Inc. https://orcid.org/0000-0001-5981-2589 en_US http://dx.doi.org/10.1089/ten.TEA.2014.0369 Tissue Engineering Part A Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Mary Ann Liebert, Inc. Mary Ann Liebert |
| spellingShingle | Lo, Justin H. Bassett, Erik K. Penson, Elliot J. N. Hoganson, David M. Vacanti, Joseph P. Gas Transfer in Cellularized Collagen-Membrane Gas Exchange Devices |
| title | Gas Transfer in Cellularized Collagen-Membrane Gas Exchange Devices |
| title_full | Gas Transfer in Cellularized Collagen-Membrane Gas Exchange Devices |
| title_fullStr | Gas Transfer in Cellularized Collagen-Membrane Gas Exchange Devices |
| title_full_unstemmed | Gas Transfer in Cellularized Collagen-Membrane Gas Exchange Devices |
| title_short | Gas Transfer in Cellularized Collagen-Membrane Gas Exchange Devices |
| title_sort | gas transfer in cellularized collagen membrane gas exchange devices |
| url | http://hdl.handle.net/1721.1/98486 https://orcid.org/0000-0001-5981-2589 |
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