CD8α[superscript +] Dendritic Cells Are the Critical Source of Interleukin-12 that Controls Acute Infection by Toxoplasma gondii Tachyzoites
CD8α[superscript +] dendritic cells (DCs) are important in vivo for cross-presentation of antigens derived from intracellular pathogens and tumors. Additionally, secretion of interleukin-12 (IL-12) by CD8α[superscript +] DCs suggests a role for these cells in response to Toxoplasma gondii antigens,...
| Autori principali: | , , , , , , , , , , |
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| Natura: | Articolo |
| Lingua: | en_US |
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Elsevier/Cell Press
2015
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| Accesso online: | http://hdl.handle.net/1721.1/99164 https://orcid.org/0000-0002-1090-6071 |
| _version_ | 1826216673823162368 |
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| author | Mashayekhi, Mona Sandau, Michelle M. Dunay, Ildiko R. Frickel, Eva M. Khan, Asis Goldszmid, Romina S. Sher, Alan Murphy, Theresa L. Sibley, L. David Murphy, Kenneth M. Ploegh, Hidde |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Mashayekhi, Mona Sandau, Michelle M. Dunay, Ildiko R. Frickel, Eva M. Khan, Asis Goldszmid, Romina S. Sher, Alan Murphy, Theresa L. Sibley, L. David Murphy, Kenneth M. Ploegh, Hidde |
| author_sort | Mashayekhi, Mona |
| collection | MIT |
| description | CD8α[superscript +] dendritic cells (DCs) are important in vivo for cross-presentation of antigens derived from intracellular pathogens and tumors. Additionally, secretion of interleukin-12 (IL-12) by CD8α[superscript +] DCs suggests a role for these cells in response to Toxoplasma gondii antigens, although it remains unclear whether these cells are required for protection against T. gondii infection. Toward this goal, we examined T. gondii infection of Batf3[superscript −/−] mice, which selectively lack only lymphoid-resident CD8α[superscript +] DCs and related peripheral CD103[superscript +] DCs. Batf3[superscript −/−] mice were extremely susceptible to T. gondii infection, with decreased production of IL-12 and interferon-γ. IL-12 administration restored resistance in Batf3[superscript −/−] mice, and mice in which IL-12 production was ablated only from CD8α[superscript +] DCs failed to control infection. These results reveal that the function of CD8α[superscript +] DCs extends beyond a role in cross-presentation and includes a critical role for activation of innate immunity through IL-12 production during T. gondii infection. |
| first_indexed | 2024-09-23T16:51:16Z |
| format | Article |
| id | mit-1721.1/99164 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:51:16Z |
| publishDate | 2015 |
| publisher | Elsevier/Cell Press |
| record_format | dspace |
| spelling | mit-1721.1/991642022-09-29T21:55:39Z CD8α[superscript +] Dendritic Cells Are the Critical Source of Interleukin-12 that Controls Acute Infection by Toxoplasma gondii Tachyzoites Mashayekhi, Mona Sandau, Michelle M. Dunay, Ildiko R. Frickel, Eva M. Khan, Asis Goldszmid, Romina S. Sher, Alan Murphy, Theresa L. Sibley, L. David Murphy, Kenneth M. Ploegh, Hidde Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research Ploegh, Hidde CD8α[superscript +] dendritic cells (DCs) are important in vivo for cross-presentation of antigens derived from intracellular pathogens and tumors. Additionally, secretion of interleukin-12 (IL-12) by CD8α[superscript +] DCs suggests a role for these cells in response to Toxoplasma gondii antigens, although it remains unclear whether these cells are required for protection against T. gondii infection. Toward this goal, we examined T. gondii infection of Batf3[superscript −/−] mice, which selectively lack only lymphoid-resident CD8α[superscript +] DCs and related peripheral CD103[superscript +] DCs. Batf3[superscript −/−] mice were extremely susceptible to T. gondii infection, with decreased production of IL-12 and interferon-γ. IL-12 administration restored resistance in Batf3[superscript −/−] mice, and mice in which IL-12 production was ablated only from CD8α[superscript +] DCs failed to control infection. These results reveal that the function of CD8α[superscript +] DCs extends beyond a role in cross-presentation and includes a critical role for activation of innate immunity through IL-12 production during T. gondii infection. 2015-10-06T18:52:06Z 2015-10-06T18:52:06Z 2011-08 2011-05 Article http://purl.org/eprint/type/JournalArticle 10747613 1097-4180 http://hdl.handle.net/1721.1/99164 Mashayekhi, Mona, Michelle M. Sandau, Ildiko R. Dunay, Eva M. Frickel, Asis Khan, Romina S. Goldszmid, Alan Sher, et al. “CD8α[superscript +] Dendritic Cells Are the Critical Source of Interleukin-12 That Controls Acute Infection by Toxoplasma Gondii Tachyzoites.” Immunity 35, no. 2 (August 2011): 249–259. https://orcid.org/0000-0002-1090-6071 en_US http://dx.doi.org/10.1016/j.immuni.2011.08.008 Immunity Creative Commons Attribution-Noncommercial-NoDerivatives http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier/Cell Press PMC |
| spellingShingle | Mashayekhi, Mona Sandau, Michelle M. Dunay, Ildiko R. Frickel, Eva M. Khan, Asis Goldszmid, Romina S. Sher, Alan Murphy, Theresa L. Sibley, L. David Murphy, Kenneth M. Ploegh, Hidde CD8α[superscript +] Dendritic Cells Are the Critical Source of Interleukin-12 that Controls Acute Infection by Toxoplasma gondii Tachyzoites |
| title | CD8α[superscript +] Dendritic Cells Are the Critical Source of Interleukin-12 that Controls Acute Infection by Toxoplasma gondii Tachyzoites |
| title_full | CD8α[superscript +] Dendritic Cells Are the Critical Source of Interleukin-12 that Controls Acute Infection by Toxoplasma gondii Tachyzoites |
| title_fullStr | CD8α[superscript +] Dendritic Cells Are the Critical Source of Interleukin-12 that Controls Acute Infection by Toxoplasma gondii Tachyzoites |
| title_full_unstemmed | CD8α[superscript +] Dendritic Cells Are the Critical Source of Interleukin-12 that Controls Acute Infection by Toxoplasma gondii Tachyzoites |
| title_short | CD8α[superscript +] Dendritic Cells Are the Critical Source of Interleukin-12 that Controls Acute Infection by Toxoplasma gondii Tachyzoites |
| title_sort | cd8α superscript dendritic cells are the critical source of interleukin 12 that controls acute infection by toxoplasma gondii tachyzoites |
| url | http://hdl.handle.net/1721.1/99164 https://orcid.org/0000-0002-1090-6071 |
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