The influence of tethered epidermal growth factor on connective tissue progenitor colony formation
Strategies to combine aspirated marrow cells with scaffolds to treat connective tissue defects are gaining increasing clinical attention and use. In situations such as large defects where initial survival and proliferation of transplanted connective tissue progenitors (CTPs) are limiting, therapeuti...
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| Format: | Article |
| Language: | en_US |
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Elsevier
2015
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| Online Access: | http://hdl.handle.net/1721.1/99188 https://orcid.org/0000-0002-1801-5548 |
| _version_ | 1826208975126790144 |
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| author | Marcantonio, Nicholas A. Boehm, Cynthia A. Rozic, Richard J. Au, Ada Wells, Alan Muschler, George F. Griffith, Linda G. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Marcantonio, Nicholas A. Boehm, Cynthia A. Rozic, Richard J. Au, Ada Wells, Alan Muschler, George F. Griffith, Linda G. |
| author_sort | Marcantonio, Nicholas A. |
| collection | MIT |
| description | Strategies to combine aspirated marrow cells with scaffolds to treat connective tissue defects are gaining increasing clinical attention and use. In situations such as large defects where initial survival and proliferation of transplanted connective tissue progenitors (CTPs) are limiting, therapeutic outcomes might be improved by using the scaffold to deliver growth factors that promote the early stages of cell function in the graft. Signaling by the epidermal growth factor receptor (EGFR) plays a role in cell survival and has been implicated in bone development and homeostasis. Providing epidermal growth factor (EGF) in a scaffold-tethered format may sustain local delivery and shift EGFR signaling to pro-survival modes compared to soluble ligand. We therefore examined the effect of tethered EGF on osteogenic colony formation from human bone marrow aspirates in the context of three different adhesion environments using a total of 39 donors. We found that tethered EGF, but not soluble EGF, increased the numbers of colonies formed regardless of adhesion background, and that tethered EGF did not impair early stages of osteogenic differentiation. |
| first_indexed | 2024-09-23T14:15:23Z |
| format | Article |
| id | mit-1721.1/99188 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:15:23Z |
| publishDate | 2015 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/991882022-09-28T19:31:04Z The influence of tethered epidermal growth factor on connective tissue progenitor colony formation Marcantonio, Nicholas A. Boehm, Cynthia A. Rozic, Richard J. Au, Ada Wells, Alan Muschler, George F. Griffith, Linda G. Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Marcantonio, Nicholas A. Au, Ada Griffith, Linda G. Strategies to combine aspirated marrow cells with scaffolds to treat connective tissue defects are gaining increasing clinical attention and use. In situations such as large defects where initial survival and proliferation of transplanted connective tissue progenitors (CTPs) are limiting, therapeutic outcomes might be improved by using the scaffold to deliver growth factors that promote the early stages of cell function in the graft. Signaling by the epidermal growth factor receptor (EGFR) plays a role in cell survival and has been implicated in bone development and homeostasis. Providing epidermal growth factor (EGF) in a scaffold-tethered format may sustain local delivery and shift EGFR signaling to pro-survival modes compared to soluble ligand. We therefore examined the effect of tethered EGF on osteogenic colony formation from human bone marrow aspirates in the context of three different adhesion environments using a total of 39 donors. We found that tethered EGF, but not soluble EGF, increased the numbers of colonies formed regardless of adhesion background, and that tethered EGF did not impair early stages of osteogenic differentiation. National Institute of General Medical Sciences (U.S.) (Grant NIH RO1 AR42997) National Institute of General Medical Sciences (U.S.) (Grant NIH RO1 AG024980) National Institute of General Medical Sciences (U.S.) (Grant NIH RO1 GM59870) National Institute of General Medical Sciences (U.S.) (Grant NIH DE019523) 2015-10-07T16:29:53Z 2015-10-07T16:29:53Z 2009-06 2009-03 Article http://purl.org/eprint/type/JournalArticle 01429612 1878-5905 http://hdl.handle.net/1721.1/99188 Marcantonio, Nicholas A., Cynthia A. Boehm, Richard J. Rozic, Ada Au, Alan Wells, George F. Muschler, and Linda G. Griffith. “The Influence of Tethered Epidermal Growth Factor on Connective Tissue Progenitor Colony Formation.” Biomaterials 30, no. 27 (September 2009): 4629–4638. https://orcid.org/0000-0002-1801-5548 en_US http://dx.doi.org/10.1016/j.biomaterials.2009.05.061 Biomaterials Creative Commons Attribution-Noncommercial-NoDerivatives http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier PMC |
| spellingShingle | Marcantonio, Nicholas A. Boehm, Cynthia A. Rozic, Richard J. Au, Ada Wells, Alan Muschler, George F. Griffith, Linda G. The influence of tethered epidermal growth factor on connective tissue progenitor colony formation |
| title | The influence of tethered epidermal growth factor on connective tissue progenitor colony formation |
| title_full | The influence of tethered epidermal growth factor on connective tissue progenitor colony formation |
| title_fullStr | The influence of tethered epidermal growth factor on connective tissue progenitor colony formation |
| title_full_unstemmed | The influence of tethered epidermal growth factor on connective tissue progenitor colony formation |
| title_short | The influence of tethered epidermal growth factor on connective tissue progenitor colony formation |
| title_sort | influence of tethered epidermal growth factor on connective tissue progenitor colony formation |
| url | http://hdl.handle.net/1721.1/99188 https://orcid.org/0000-0002-1801-5548 |
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