Stress-vs-time signals allow the prediction of structurally catastrophic events during fracturing of immature cartilage and predetermine the biomechanical, biochemical, and structural impairment
Objective Trauma-associated cartilage fractures occur in children and adolescents with clinically significant incidence. Several studies investigated biomechanical injury by compressive forces but the injury-related stress has not been investigated extensively. In this study, we hypothesized that t...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Elsevier
2015
|
| Online Access: | http://hdl.handle.net/1721.1/99422 https://orcid.org/0000-0002-4942-3456 |
| _version_ | 1811085329906532352 |
|---|---|
| author | Rolauffs, Bernd Kurz, Bodo Felka, Tino Rothdiener, Miriam Uynuk-Ool, Tatiana Aurich, Matthias Frank, Eliot Bahrs, Christian Badke, Andreas Stöckle, Ulrich Aicher, Wilhelm K. Grodzinsky, Alan J. |
| author2 | Massachusetts Institute of Technology. Center for Biomedical Engineering |
| author_facet | Massachusetts Institute of Technology. Center for Biomedical Engineering Rolauffs, Bernd Kurz, Bodo Felka, Tino Rothdiener, Miriam Uynuk-Ool, Tatiana Aurich, Matthias Frank, Eliot Bahrs, Christian Badke, Andreas Stöckle, Ulrich Aicher, Wilhelm K. Grodzinsky, Alan J. |
| author_sort | Rolauffs, Bernd |
| collection | MIT |
| description | Objective
Trauma-associated cartilage fractures occur in children and adolescents with clinically significant incidence. Several studies investigated biomechanical injury by compressive forces but the injury-related stress has not been investigated extensively. In this study, we hypothesized that the biomechanical stress occurring during compressive injury predetermines the biomechanical, biochemical, and structural consequences. We specifically investigated whether the stress-vs-time signal correlated with the injurious damage and may allow prediction of cartilage matrix fracturing.
Methods
Superficial and deeper zones disks (SZDs, DZDs; immature bovine cartilage) were biomechanically characterized, injured (50% compression, 100%/s strain-rate), and re-characterized. Correlations of the quantified functional, biochemical and histological damage with biomechanical parameters were zonally investigated.
Results
Injured SZDs exhibited decreased dynamic stiffness (by 93.04 ± 1.72%), unresolvable equilibrium moduli, structural damage (2.0 ± 0.5 on a 5-point-damage-scale), and 1.78-fold increased sGAG loss. DZDs remained intact. Measured stress-vs-time-curves during injury displayed 4 distinct shapes, which correlated with histological damage (p < 0.001), loss of dynamic stiffness and sGAG (p < 0.05). Damage prediction in a blinded experiment using stress-vs-time grades was 100%-correct and sensitive to differentiate single/complex matrix disruptions. Correlations of the dissipated energy and maximum stress rise with the extent of biomechanical and biochemical damage reached significance when SZDs and DZDs were analyzed as zonal composites but not separately.
Conclusions
The biomechanical stress that occurs during compressive injury predetermines the biomechanical, biochemical, and structural consequences and, thus, the structural and functional damage during cartilage fracturing. A novel biomechanical method based on the interpretation of compressive yielding allows the accurate prediction of the extent of structural damage. |
| first_indexed | 2024-09-23T13:07:12Z |
| format | Article |
| id | mit-1721.1/99422 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:07:12Z |
| publishDate | 2015 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | mit-1721.1/994222022-10-01T13:11:21Z Stress-vs-time signals allow the prediction of structurally catastrophic events during fracturing of immature cartilage and predetermine the biomechanical, biochemical, and structural impairment Rolauffs, Bernd Kurz, Bodo Felka, Tino Rothdiener, Miriam Uynuk-Ool, Tatiana Aurich, Matthias Frank, Eliot Bahrs, Christian Badke, Andreas Stöckle, Ulrich Aicher, Wilhelm K. Grodzinsky, Alan J. Massachusetts Institute of Technology. Center for Biomedical Engineering Massachusetts Institute of Technology. Department of Biological Engineering Rolauffs, Bernd Frank, Eliot Grodzinsky, Alan J. Objective Trauma-associated cartilage fractures occur in children and adolescents with clinically significant incidence. Several studies investigated biomechanical injury by compressive forces but the injury-related stress has not been investigated extensively. In this study, we hypothesized that the biomechanical stress occurring during compressive injury predetermines the biomechanical, biochemical, and structural consequences. We specifically investigated whether the stress-vs-time signal correlated with the injurious damage and may allow prediction of cartilage matrix fracturing. Methods Superficial and deeper zones disks (SZDs, DZDs; immature bovine cartilage) were biomechanically characterized, injured (50% compression, 100%/s strain-rate), and re-characterized. Correlations of the quantified functional, biochemical and histological damage with biomechanical parameters were zonally investigated. Results Injured SZDs exhibited decreased dynamic stiffness (by 93.04 ± 1.72%), unresolvable equilibrium moduli, structural damage (2.0 ± 0.5 on a 5-point-damage-scale), and 1.78-fold increased sGAG loss. DZDs remained intact. Measured stress-vs-time-curves during injury displayed 4 distinct shapes, which correlated with histological damage (p < 0.001), loss of dynamic stiffness and sGAG (p < 0.05). Damage prediction in a blinded experiment using stress-vs-time grades was 100%-correct and sensitive to differentiate single/complex matrix disruptions. Correlations of the dissipated energy and maximum stress rise with the extent of biomechanical and biochemical damage reached significance when SZDs and DZDs were analyzed as zonal composites but not separately. Conclusions The biomechanical stress that occurs during compressive injury predetermines the biomechanical, biochemical, and structural consequences and, thus, the structural and functional damage during cartilage fracturing. A novel biomechanical method based on the interpretation of compressive yielding allows the accurate prediction of the extent of structural damage. National Institutes of Health (U.S.) (Grant R01-AR45779) Deutsche Forschungsgemeinschaft (Grant RO2511/1-1) Deutsche Forschungsgemeinschaft (Grant RO2511/2-1) Germany. Federal Ministry of Education and Research (Grant 01KQ0902B TP2) 2015-10-23T13:04:17Z 2015-10-23T13:04:17Z 2013-06 2013-06 Article http://purl.org/eprint/type/JournalArticle 10478477 1095-8657 http://hdl.handle.net/1721.1/99422 Rolauffs, Bernd, Bodo Kurz, Tino Felka, Miriam Rothdiener, Tatiana Uynuk-Ool, Matthias Aurich, Eliot Frank, et al. “Stress-Vs-Time Signals Allow the Prediction of Structurally Catastrophic Events During Fracturing of Immature Cartilage and Predetermine the Biomechanical, Biochemical, and Structural Impairment.” Journal of Structural Biology 183, no. 3 (September 2013): 501–511. https://orcid.org/0000-0002-4942-3456 en_US http://dx.doi.org/10.1016/j.jsb.2013.06.011 Journal of Structural Biology Creative Commons Attribution http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf Elsevier PMC |
| spellingShingle | Rolauffs, Bernd Kurz, Bodo Felka, Tino Rothdiener, Miriam Uynuk-Ool, Tatiana Aurich, Matthias Frank, Eliot Bahrs, Christian Badke, Andreas Stöckle, Ulrich Aicher, Wilhelm K. Grodzinsky, Alan J. Stress-vs-time signals allow the prediction of structurally catastrophic events during fracturing of immature cartilage and predetermine the biomechanical, biochemical, and structural impairment |
| title | Stress-vs-time signals allow the prediction of structurally catastrophic events during fracturing of immature cartilage and predetermine the biomechanical, biochemical, and structural impairment |
| title_full | Stress-vs-time signals allow the prediction of structurally catastrophic events during fracturing of immature cartilage and predetermine the biomechanical, biochemical, and structural impairment |
| title_fullStr | Stress-vs-time signals allow the prediction of structurally catastrophic events during fracturing of immature cartilage and predetermine the biomechanical, biochemical, and structural impairment |
| title_full_unstemmed | Stress-vs-time signals allow the prediction of structurally catastrophic events during fracturing of immature cartilage and predetermine the biomechanical, biochemical, and structural impairment |
| title_short | Stress-vs-time signals allow the prediction of structurally catastrophic events during fracturing of immature cartilage and predetermine the biomechanical, biochemical, and structural impairment |
| title_sort | stress vs time signals allow the prediction of structurally catastrophic events during fracturing of immature cartilage and predetermine the biomechanical biochemical and structural impairment |
| url | http://hdl.handle.net/1721.1/99422 https://orcid.org/0000-0002-4942-3456 |
| work_keys_str_mv | AT rolauffsbernd stressvstimesignalsallowthepredictionofstructurallycatastrophiceventsduringfracturingofimmaturecartilageandpredeterminethebiomechanicalbiochemicalandstructuralimpairment AT kurzbodo stressvstimesignalsallowthepredictionofstructurallycatastrophiceventsduringfracturingofimmaturecartilageandpredeterminethebiomechanicalbiochemicalandstructuralimpairment AT felkatino stressvstimesignalsallowthepredictionofstructurallycatastrophiceventsduringfracturingofimmaturecartilageandpredeterminethebiomechanicalbiochemicalandstructuralimpairment AT rothdienermiriam stressvstimesignalsallowthepredictionofstructurallycatastrophiceventsduringfracturingofimmaturecartilageandpredeterminethebiomechanicalbiochemicalandstructuralimpairment AT uynukooltatiana stressvstimesignalsallowthepredictionofstructurallycatastrophiceventsduringfracturingofimmaturecartilageandpredeterminethebiomechanicalbiochemicalandstructuralimpairment AT aurichmatthias stressvstimesignalsallowthepredictionofstructurallycatastrophiceventsduringfracturingofimmaturecartilageandpredeterminethebiomechanicalbiochemicalandstructuralimpairment AT frankeliot stressvstimesignalsallowthepredictionofstructurallycatastrophiceventsduringfracturingofimmaturecartilageandpredeterminethebiomechanicalbiochemicalandstructuralimpairment AT bahrschristian stressvstimesignalsallowthepredictionofstructurallycatastrophiceventsduringfracturingofimmaturecartilageandpredeterminethebiomechanicalbiochemicalandstructuralimpairment AT badkeandreas stressvstimesignalsallowthepredictionofstructurallycatastrophiceventsduringfracturingofimmaturecartilageandpredeterminethebiomechanicalbiochemicalandstructuralimpairment AT stockleulrich stressvstimesignalsallowthepredictionofstructurallycatastrophiceventsduringfracturingofimmaturecartilageandpredeterminethebiomechanicalbiochemicalandstructuralimpairment AT aicherwilhelmk stressvstimesignalsallowthepredictionofstructurallycatastrophiceventsduringfracturingofimmaturecartilageandpredeterminethebiomechanicalbiochemicalandstructuralimpairment AT grodzinskyalanj stressvstimesignalsallowthepredictionofstructurallycatastrophiceventsduringfracturingofimmaturecartilageandpredeterminethebiomechanicalbiochemicalandstructuralimpairment |