Prediction of cartilage compressive modulus using multiexponential analysis of T[subscript 2] relaxation data and support vector regression
Evaluation of mechanical characteristics of cartilage by magnetic resonance imaging would provide a noninvasive measure of tissue quality both for tissue engineering and when monitoring clinical response to therapeutic interventions for cartilage degradation. We use results from multiexponential tra...
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| Format: | Article |
| Language: | en_US |
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Wiley Blackwell
2015
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| Online Access: | http://hdl.handle.net/1721.1/99427 https://orcid.org/0000-0002-4942-3456 |
| _version_ | 1811087077400379392 |
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| author | Irrechukwu, Onyi N. Thaer, Sarah Von Lin, Ping-Chang Reiter, David A. Grodzinsky, Alan J. Spencer, Richard G. Frank, Eliot |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Irrechukwu, Onyi N. Thaer, Sarah Von Lin, Ping-Chang Reiter, David A. Grodzinsky, Alan J. Spencer, Richard G. Frank, Eliot |
| author_sort | Irrechukwu, Onyi N. |
| collection | MIT |
| description | Evaluation of mechanical characteristics of cartilage by magnetic resonance imaging would provide a noninvasive measure of tissue quality both for tissue engineering and when monitoring clinical response to therapeutic interventions for cartilage degradation. We use results from multiexponential transverse relaxation analysis to predict equilibrium and dynamic stiffness of control and degraded bovine nasal cartilage, a biochemical model for articular cartilage. Sulfated glycosaminoglycan concentration/wet weight (ww) and equilibrium and dynamic stiffness decreased with degradation from 103.6 ± 37.0 µg/mg ww, 1.71 ± 1.10 MPa and 15.3 ± 6.7 MPa in controls to 8.25 ± 2.4 µg/mg ww, 0.015 ± 0.006 MPa and 0.89 ± 0.25MPa, respectively, in severely degraded explants. Magnetic resonance measurements were performed on cartilage explants at 4 °C in a 9.4 T wide-bore NMR spectrometer using a Carr–Purcell–Meiboom–Gill sequence. Multiexponential T[subscript 2] analysis revealed four water compartments with T[subscript 2] values of approximately 0.14, 3, 40 and 150 ms, with corresponding weight fractions of approximately 3, 2, 4 and 91%. Correlations between weight fractions and stiffness based on conventional univariate and multiple linear regressions exhibited a maximum r[superscript 2] of 0.65, while those based on support vector regression (SVR) had a maximum r[superscript 2] value of 0.90. These results indicate that (i) compartment weight fractions derived from multiexponential analysis reflect cartilage stiffness and (ii) SVR-based multivariate regression exhibits greatly improved accuracy in predicting mechanical properties as compared with conventional regression. |
| first_indexed | 2024-09-23T13:39:25Z |
| format | Article |
| id | mit-1721.1/99427 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:39:25Z |
| publishDate | 2015 |
| publisher | Wiley Blackwell |
| record_format | dspace |
| spelling | mit-1721.1/994272022-10-01T16:20:00Z Prediction of cartilage compressive modulus using multiexponential analysis of T[subscript 2] relaxation data and support vector regression Irrechukwu, Onyi N. Thaer, Sarah Von Lin, Ping-Chang Reiter, David A. Grodzinsky, Alan J. Spencer, Richard G. Frank, Eliot Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Frank, Eliot Grodzinsky, Alan J. Evaluation of mechanical characteristics of cartilage by magnetic resonance imaging would provide a noninvasive measure of tissue quality both for tissue engineering and when monitoring clinical response to therapeutic interventions for cartilage degradation. We use results from multiexponential transverse relaxation analysis to predict equilibrium and dynamic stiffness of control and degraded bovine nasal cartilage, a biochemical model for articular cartilage. Sulfated glycosaminoglycan concentration/wet weight (ww) and equilibrium and dynamic stiffness decreased with degradation from 103.6 ± 37.0 µg/mg ww, 1.71 ± 1.10 MPa and 15.3 ± 6.7 MPa in controls to 8.25 ± 2.4 µg/mg ww, 0.015 ± 0.006 MPa and 0.89 ± 0.25MPa, respectively, in severely degraded explants. Magnetic resonance measurements were performed on cartilage explants at 4 °C in a 9.4 T wide-bore NMR spectrometer using a Carr–Purcell–Meiboom–Gill sequence. Multiexponential T[subscript 2] analysis revealed four water compartments with T[subscript 2] values of approximately 0.14, 3, 40 and 150 ms, with corresponding weight fractions of approximately 3, 2, 4 and 91%. Correlations between weight fractions and stiffness based on conventional univariate and multiple linear regressions exhibited a maximum r[superscript 2] of 0.65, while those based on support vector regression (SVR) had a maximum r[superscript 2] value of 0.90. These results indicate that (i) compartment weight fractions derived from multiexponential analysis reflect cartilage stiffness and (ii) SVR-based multivariate regression exhibits greatly improved accuracy in predicting mechanical properties as compared with conventional regression. National Institutes of Health (U.S.). Intramural Research Program National Institute on Aging 2015-10-23T13:58:34Z 2015-10-23T13:58:34Z 2014-02 Article http://purl.org/eprint/type/JournalArticle 09523480 1099-1492 http://hdl.handle.net/1721.1/99427 Irrechukwu, Onyi N., Sarah Von Thaer, Eliot H. Frank, Ping-Chang Lin, David A. Reiter, Alan J. Grodzinsky, and Richard G. Spencer. “Prediction of Cartilage Compressive Modulus Using Multiexponential Analysis of T[subscript 2] Relaxation Data and Support Vector Regression.” NMR Biomed. 27, no. 4 (February 12, 2014): 468–477. https://orcid.org/0000-0002-4942-3456 en_US http://dx.doi.org/10.1002/nbm.3083 NMR in Biomedicine Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Wiley Blackwell PMC |
| spellingShingle | Irrechukwu, Onyi N. Thaer, Sarah Von Lin, Ping-Chang Reiter, David A. Grodzinsky, Alan J. Spencer, Richard G. Frank, Eliot Prediction of cartilage compressive modulus using multiexponential analysis of T[subscript 2] relaxation data and support vector regression |
| title | Prediction of cartilage compressive modulus using multiexponential analysis of T[subscript 2] relaxation data and support vector regression |
| title_full | Prediction of cartilage compressive modulus using multiexponential analysis of T[subscript 2] relaxation data and support vector regression |
| title_fullStr | Prediction of cartilage compressive modulus using multiexponential analysis of T[subscript 2] relaxation data and support vector regression |
| title_full_unstemmed | Prediction of cartilage compressive modulus using multiexponential analysis of T[subscript 2] relaxation data and support vector regression |
| title_short | Prediction of cartilage compressive modulus using multiexponential analysis of T[subscript 2] relaxation data and support vector regression |
| title_sort | prediction of cartilage compressive modulus using multiexponential analysis of t subscript 2 relaxation data and support vector regression |
| url | http://hdl.handle.net/1721.1/99427 https://orcid.org/0000-0002-4942-3456 |
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