Measuring single cell mass, volume, and density with dual suspended microchannel resonators
Cell size, measured as either volume or mass, is a fundamental indicator of cell state. Far more tightly regulated than size is density, the ratio between mass and volume, which can be used to distinguish between cell populations even when volume and mass appear to remain constant. Here we expand up...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | en_US |
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Royal Society of Chemistry
2015
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| Online Access: | http://hdl.handle.net/1721.1/99500 https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0003-4110-1388 |
| _version_ | 1811087633972985856 |
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| author | Shen, Wenjiang Grover, William H. Hecht, Vivian Chaya Payer, Kristofor Robert Bryan, Andrea Kristine Manalis, Scott R |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Shen, Wenjiang Grover, William H. Hecht, Vivian Chaya Payer, Kristofor Robert Bryan, Andrea Kristine Manalis, Scott R |
| author_sort | Shen, Wenjiang |
| collection | MIT |
| description | Cell size, measured as either volume or mass, is a fundamental indicator of cell state. Far more tightly regulated than size is density, the ratio between mass and volume, which can be used to distinguish between cell populations even when volume and mass appear to remain constant. Here we expand upon a previous method for measuring cell density involving a suspended microchannel resonator (SMR). We introduce a new device, the dual SMR, as a high-precision instrument for measuring single-cell mass, volume, and density using two resonators connected by a serpentine fluidic channel. The dual SMR designs considered herein demonstrate the critical role of channel geometry in ensuring proper mixing and damping of pressure fluctuations in microfluidic systems designed for precision measurement. We use the dual SMR to compare the physical properties of two well-known cancer cell lines: human lung cancer cell H1650 and mouse lymphoblastic leukemia cell line L1210. |
| first_indexed | 2024-09-23T13:49:34Z |
| format | Article |
| id | mit-1721.1/99500 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:49:34Z |
| publishDate | 2015 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | mit-1721.1/995002022-09-28T16:25:20Z Measuring single cell mass, volume, and density with dual suspended microchannel resonators Shen, Wenjiang Grover, William H. Hecht, Vivian Chaya Payer, Kristofor Robert Bryan, Andrea Kristine Manalis, Scott R Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Microsystems Technology Laboratories Koch Institute for Integrative Cancer Research at MIT Bryan, Andrea K. Hecht, Vivian Chaya Payer, Kristofor Robert Grover, William H. Manalis, Scott R. Cell size, measured as either volume or mass, is a fundamental indicator of cell state. Far more tightly regulated than size is density, the ratio between mass and volume, which can be used to distinguish between cell populations even when volume and mass appear to remain constant. Here we expand upon a previous method for measuring cell density involving a suspended microchannel resonator (SMR). We introduce a new device, the dual SMR, as a high-precision instrument for measuring single-cell mass, volume, and density using two resonators connected by a serpentine fluidic channel. The dual SMR designs considered herein demonstrate the critical role of channel geometry in ensuring proper mixing and damping of pressure fluctuations in microfluidic systems designed for precision measurement. We use the dual SMR to compare the physical properties of two well-known cancer cell lines: human lung cancer cell H1650 and mouse lymphoblastic leukemia cell line L1210. National Cancer Institute (U.S.) (Koch Institute Support (Core) Grant P30-CA14051) National Cancer Institute (U.S.). Physical Sciences Oncology Center (U54CA143874) National Cancer Institute (U.S.). Cell Decision Process Center (P50GM68762) National Institutes of Health (U.S.) (Contract R01GM085457) 2015-10-29T14:55:25Z 2015-10-29T14:55:25Z 2013-12 2013-09 Article http://purl.org/eprint/type/JournalArticle 1473-0197 1473-0189 http://hdl.handle.net/1721.1/99500 Bryan, Andrea K., Vivian C. Hecht, Wenjiang Shen, Kristofor Payer, William H. Grover, and Scott R. Manalis. “Measuring Single Cell Mass, Volume, and Density with Dual Suspended Microchannel Resonators.” Lab Chip 14, no. 3 (2014): 569–576. https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0003-4110-1388 en_US http://dx.doi.org/10.1039/c3lc51022k Lab on a Chip Creative Commons Attribution-Noncommercial-Share Alike http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Royal Society of Chemistry PMC |
| spellingShingle | Shen, Wenjiang Grover, William H. Hecht, Vivian Chaya Payer, Kristofor Robert Bryan, Andrea Kristine Manalis, Scott R Measuring single cell mass, volume, and density with dual suspended microchannel resonators |
| title | Measuring single cell mass, volume, and density with dual suspended microchannel resonators |
| title_full | Measuring single cell mass, volume, and density with dual suspended microchannel resonators |
| title_fullStr | Measuring single cell mass, volume, and density with dual suspended microchannel resonators |
| title_full_unstemmed | Measuring single cell mass, volume, and density with dual suspended microchannel resonators |
| title_short | Measuring single cell mass, volume, and density with dual suspended microchannel resonators |
| title_sort | measuring single cell mass volume and density with dual suspended microchannel resonators |
| url | http://hdl.handle.net/1721.1/99500 https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0003-4110-1388 |
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