Pathogenesis and transmission of swine origin A(H3N2)v influenza viruses in ferrets
Recent isolation of a novel swine-origin influenza A H3N2 variant virus [A(H3N2)v] from humans in the United States has raised concern over the pandemic potential of these viruses. Here, we analyzed the virulence, transmissibility, and receptor-binding preference of four A(H3N2)v influenza viruses i...
| Main Authors: | , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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National Academy of Sciences (U.S.)
2015
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| Online Access: | http://hdl.handle.net/1721.1/99517 https://orcid.org/0000-0002-2085-7840 |
| _version_ | 1826193998209875968 |
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| author | Pearce, Melissa B. Jayaraman, Akila Pappas, Claudia Belser, Jessica A. Zeng, Hui Gustin, Kortney M. Maines, Taronna R. Sun, Xiangjie Raman, Rahul Cox, Nancy J. Sasisekharan, Ram Katz, Jacqueline M. Tumpey, Terrence M. |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Pearce, Melissa B. Jayaraman, Akila Pappas, Claudia Belser, Jessica A. Zeng, Hui Gustin, Kortney M. Maines, Taronna R. Sun, Xiangjie Raman, Rahul Cox, Nancy J. Sasisekharan, Ram Katz, Jacqueline M. Tumpey, Terrence M. |
| author_sort | Pearce, Melissa B. |
| collection | MIT |
| description | Recent isolation of a novel swine-origin influenza A H3N2 variant virus [A(H3N2)v] from humans in the United States has raised concern over the pandemic potential of these viruses. Here, we analyzed the virulence, transmissibility, and receptor-binding preference of four A(H3N2)v influenza viruses isolated from humans in 2009, 2010, and 2011. High titers of infectious virus were detected in nasal turbinates and nasal wash samples of A(H3N2)v-inoculated ferrets. All four A(H3N2)v viruses possessed the capacity to spread efficiently between cohoused ferrets, and the 2010 and 2011 A(H3N2)v isolates transmitted efficiently to naïve ferrets by respiratory droplets. A dose-dependent glycan array analysis of A(H3N2)v showed a predominant binding to α2-6–sialylated glycans, similar to human-adapted influenza A viruses. We further tested the viral replication efficiency of A(H3N2)v viruses in a relevant cell line, Calu-3, derived from human bronchial epithelium. The A(H3N2)v viruses replicated in Calu-3 cells to significantly higher titers compared with five common seasonal H3N2 influenza viruses. These findings suggest that A(H3N2)v viruses have the capacity for efficient replication and transmission in mammals and underscore the need for continued public health surveillance. |
| first_indexed | 2024-09-23T09:48:58Z |
| format | Article |
| id | mit-1721.1/99517 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:48:58Z |
| publishDate | 2015 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/995172022-09-26T13:53:32Z Pathogenesis and transmission of swine origin A(H3N2)v influenza viruses in ferrets Pearce, Melissa B. Jayaraman, Akila Pappas, Claudia Belser, Jessica A. Zeng, Hui Gustin, Kortney M. Maines, Taronna R. Sun, Xiangjie Raman, Rahul Cox, Nancy J. Sasisekharan, Ram Katz, Jacqueline M. Tumpey, Terrence M. Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. School of Engineering Koch Institute for Integrative Cancer Research at MIT Jayaraman, Akila Raman, Rahul Sasisekharan, Ram Recent isolation of a novel swine-origin influenza A H3N2 variant virus [A(H3N2)v] from humans in the United States has raised concern over the pandemic potential of these viruses. Here, we analyzed the virulence, transmissibility, and receptor-binding preference of four A(H3N2)v influenza viruses isolated from humans in 2009, 2010, and 2011. High titers of infectious virus were detected in nasal turbinates and nasal wash samples of A(H3N2)v-inoculated ferrets. All four A(H3N2)v viruses possessed the capacity to spread efficiently between cohoused ferrets, and the 2010 and 2011 A(H3N2)v isolates transmitted efficiently to naïve ferrets by respiratory droplets. A dose-dependent glycan array analysis of A(H3N2)v showed a predominant binding to α2-6–sialylated glycans, similar to human-adapted influenza A viruses. We further tested the viral replication efficiency of A(H3N2)v viruses in a relevant cell line, Calu-3, derived from human bronchial epithelium. The A(H3N2)v viruses replicated in Calu-3 cells to significantly higher titers compared with five common seasonal H3N2 influenza viruses. These findings suggest that A(H3N2)v viruses have the capacity for efficient replication and transmission in mammals and underscore the need for continued public health surveillance. National Institutes of Health (U.S.) (GM 57073) Singapore-MIT Alliance for Research and Technology 2015-10-29T18:22:15Z 2015-10-29T18:22:15Z 2012-02 2011-12 Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/99517 Pearce, M. B., A. Jayaraman, C. Pappas, J. A. Belser, H. Zeng, K. M. Gustin, T. R. Maines, et al. “Pathogenesis and Transmission of Swine Origin A(H3N2)v Influenza Viruses in Ferrets.” Proceedings of the National Academy of Sciences 109, no. 10 (February 21, 2012): 3944–3949. https://orcid.org/0000-0002-2085-7840 en_US http://dx.doi.org/10.1073/pnas.1119945109 Proceedings of the National Academy of Sciences Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) PNAS |
| spellingShingle | Pearce, Melissa B. Jayaraman, Akila Pappas, Claudia Belser, Jessica A. Zeng, Hui Gustin, Kortney M. Maines, Taronna R. Sun, Xiangjie Raman, Rahul Cox, Nancy J. Sasisekharan, Ram Katz, Jacqueline M. Tumpey, Terrence M. Pathogenesis and transmission of swine origin A(H3N2)v influenza viruses in ferrets |
| title | Pathogenesis and transmission of swine origin A(H3N2)v influenza viruses in ferrets |
| title_full | Pathogenesis and transmission of swine origin A(H3N2)v influenza viruses in ferrets |
| title_fullStr | Pathogenesis and transmission of swine origin A(H3N2)v influenza viruses in ferrets |
| title_full_unstemmed | Pathogenesis and transmission of swine origin A(H3N2)v influenza viruses in ferrets |
| title_short | Pathogenesis and transmission of swine origin A(H3N2)v influenza viruses in ferrets |
| title_sort | pathogenesis and transmission of swine origin a h3n2 v influenza viruses in ferrets |
| url | http://hdl.handle.net/1721.1/99517 https://orcid.org/0000-0002-2085-7840 |
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