Tunable Membranes for Free-Flow Zone Electrophoresis in PDMS Microchip Using Guided Self-Assembly of Silica Microbeads
In this paper, we evaluate the strategy of using self-assembled microbeads to build a robust and tunable membrane for free-flow zone electrophoresis in a PDMS microfluidic chip. To fabricate a porous membrane as a salt bridge for free-flow zone electrophoresis, we used silica or polystyrene microbea...
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| Format: | Article |
| Language: | en_US |
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American Chemical Society (ACS)
2015
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| Online Access: | http://hdl.handle.net/1721.1/99520 https://orcid.org/0000-0002-2325-552X https://orcid.org/0000-0001-7215-1439 https://orcid.org/0000-0003-3150-6170 |
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| author | Song, Yong-Ak Wu, Lidan Wishnok, John S. Han, Jongyoon Tannenbaum, Steven Robert |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Song, Yong-Ak Wu, Lidan Wishnok, John S. Han, Jongyoon Tannenbaum, Steven Robert |
| author_sort | Song, Yong-Ak |
| collection | MIT |
| description | In this paper, we evaluate the strategy of using self-assembled microbeads to build a robust and tunable membrane for free-flow zone electrophoresis in a PDMS microfluidic chip. To fabricate a porous membrane as a salt bridge for free-flow zone electrophoresis, we used silica or polystyrene microbeads between 3–6 μm in diameter and packed them inside a microchannel. After complete evaporation, we infiltrated the porous microbead structure with a positively or negatively charged hydrogel to modify its surface charge polarity. Using this device, we demonstrated binary sorting (separation of positive and negative species at a given pH) of peptides and dyes in standard buffer systems without using sheath flows. The sample loss during sorting could be minimized by using ion selectivity of hydrogel-infiltrated microbead membranes. Our fabrication method enables building a robust membrane for pressure-driven free-flow zone electrophoresis with tunable pore size as well as surface charge polarity. |
| first_indexed | 2024-09-23T15:11:34Z |
| format | Article |
| id | mit-1721.1/99520 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:11:34Z |
| publishDate | 2015 |
| publisher | American Chemical Society (ACS) |
| record_format | dspace |
| spelling | mit-1721.1/995202022-10-02T01:14:15Z Tunable Membranes for Free-Flow Zone Electrophoresis in PDMS Microchip Using Guided Self-Assembly of Silica Microbeads Song, Yong-Ak Wu, Lidan Wishnok, John S. Han, Jongyoon Tannenbaum, Steven Robert Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemistry Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Song, Yong-Ak Wu, Lidan Tannenbaum, Steven Robert Wishnok, John S. Han, Jongyoon In this paper, we evaluate the strategy of using self-assembled microbeads to build a robust and tunable membrane for free-flow zone electrophoresis in a PDMS microfluidic chip. To fabricate a porous membrane as a salt bridge for free-flow zone electrophoresis, we used silica or polystyrene microbeads between 3–6 μm in diameter and packed them inside a microchannel. After complete evaporation, we infiltrated the porous microbead structure with a positively or negatively charged hydrogel to modify its surface charge polarity. Using this device, we demonstrated binary sorting (separation of positive and negative species at a given pH) of peptides and dyes in standard buffer systems without using sheath flows. The sample loss during sorting could be minimized by using ion selectivity of hydrogel-infiltrated microbead membranes. Our fabrication method enables building a robust membrane for pressure-driven free-flow zone electrophoresis with tunable pore size as well as surface charge polarity. National Institutes of Health (U.S.) (R21 EB008177-01A2) National Institutes of Health (U.S.) (P30-ES002109) 2015-10-29T18:39:12Z 2015-10-29T18:39:12Z 2013-11 2013-07 Article http://purl.org/eprint/type/JournalArticle 0003-2700 1520-6882 http://hdl.handle.net/1721.1/99520 Song, Yong-Ak, Lidan Wu, Steven R. Tannenbaum, John S. Wishnok, and Jongyoon Han. “Tunable Membranes for Free-Flow Zone Electrophoresis in PDMS Microchip Using Guided Self-Assembly of Silica Microbeads.” Anal. Chem. 85, no. 24 (December 17, 2013): 11695–11699. https://orcid.org/0000-0002-2325-552X https://orcid.org/0000-0001-7215-1439 https://orcid.org/0000-0003-3150-6170 en_US http://dx.doi.org/10.1021/ac402169x Analytical Chemistry Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society (ACS) PMC |
| spellingShingle | Song, Yong-Ak Wu, Lidan Wishnok, John S. Han, Jongyoon Tannenbaum, Steven Robert Tunable Membranes for Free-Flow Zone Electrophoresis in PDMS Microchip Using Guided Self-Assembly of Silica Microbeads |
| title | Tunable Membranes for Free-Flow Zone Electrophoresis in PDMS Microchip Using Guided Self-Assembly of Silica Microbeads |
| title_full | Tunable Membranes for Free-Flow Zone Electrophoresis in PDMS Microchip Using Guided Self-Assembly of Silica Microbeads |
| title_fullStr | Tunable Membranes for Free-Flow Zone Electrophoresis in PDMS Microchip Using Guided Self-Assembly of Silica Microbeads |
| title_full_unstemmed | Tunable Membranes for Free-Flow Zone Electrophoresis in PDMS Microchip Using Guided Self-Assembly of Silica Microbeads |
| title_short | Tunable Membranes for Free-Flow Zone Electrophoresis in PDMS Microchip Using Guided Self-Assembly of Silica Microbeads |
| title_sort | tunable membranes for free flow zone electrophoresis in pdms microchip using guided self assembly of silica microbeads |
| url | http://hdl.handle.net/1721.1/99520 https://orcid.org/0000-0002-2325-552X https://orcid.org/0000-0001-7215-1439 https://orcid.org/0000-0003-3150-6170 |
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