Antibody Derived Peptides for Detection of Ebola Virus Glycoprotein
Background Current Ebola virus (EBOV) detection methods are costly and impractical for epidemic scenarios. Different immune-based assays have been reported for the detection and quantification of Ebola virus (EBOV) proteins. In particular, several monoclonal antibodies (mAbs) have been described th...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Public Library of Science
2015
|
| Online Access: | http://hdl.handle.net/1721.1/99735 |
| _version_ | 1826195749328650240 |
|---|---|
| author | Rodriguez-Martinez, Luis Mario Marquez-Ipina, Alan Roberto Lopez-Pacheco, Felipe Perez-Chavarria, Roberto Gonzalez-Vazquez, Juan Carlos Gonzalez-Gonzalez, Everardo Trujillo-de Santiago, Grissel Ponce-Ponce de Leon, Cesar Alejandro Zhang, Yu Shrike Dokmeci, Mehmet Remzi Khademhosseini, Ali Alvarez, Mario Moises |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Rodriguez-Martinez, Luis Mario Marquez-Ipina, Alan Roberto Lopez-Pacheco, Felipe Perez-Chavarria, Roberto Gonzalez-Vazquez, Juan Carlos Gonzalez-Gonzalez, Everardo Trujillo-de Santiago, Grissel Ponce-Ponce de Leon, Cesar Alejandro Zhang, Yu Shrike Dokmeci, Mehmet Remzi Khademhosseini, Ali Alvarez, Mario Moises |
| author_sort | Rodriguez-Martinez, Luis Mario |
| collection | MIT |
| description | Background
Current Ebola virus (EBOV) detection methods are costly and impractical for epidemic scenarios. Different immune-based assays have been reported for the detection and quantification of Ebola virus (EBOV) proteins. In particular, several monoclonal antibodies (mAbs) have been described that bind the capsid glycoprotein (GP) of EBOV GP. However, the currently available platforms for the design and production of full-length mAbs are cumbersome and costly. The use of antibody fragments, rather than full-length antibodies, might represent a cost-effective alternative for the development of diagnostic and possibly even therapeutic alternatives for EBOV.
Methods/Principal Findings
We report the design and expression of three recombinant anti-GP mAb fragments in Escherichia coli cultures. These fragments contained the heavy and light variable portions of the three well-studied anti-GP full-length mAbs 13C6, 13F6, and KZ52, and are consequently named scFv-13C6, scFv-13F6, and Fab-KZ52, respectively. All three fragments exhibited specific anti-GP binding activity in ELISA experiments comparable to that of full-length anti-GP antibodies (i.e., the same order of magnitude) and they are easily and economically produced in bacterial cultures.
Conclusion/Significance
Antibody fragments might represent a useful, effective, and low cost alternative to full-length antibodies in Ebola related capture and diagnostics applications. |
| first_indexed | 2024-09-23T10:14:50Z |
| format | Article |
| id | mit-1721.1/99735 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:14:50Z |
| publishDate | 2015 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/997352022-09-30T19:52:16Z Antibody Derived Peptides for Detection of Ebola Virus Glycoprotein Rodriguez-Martinez, Luis Mario Marquez-Ipina, Alan Roberto Lopez-Pacheco, Felipe Perez-Chavarria, Roberto Gonzalez-Vazquez, Juan Carlos Gonzalez-Gonzalez, Everardo Trujillo-de Santiago, Grissel Ponce-Ponce de Leon, Cesar Alejandro Zhang, Yu Shrike Dokmeci, Mehmet Remzi Khademhosseini, Ali Alvarez, Mario Moises Harvard University--MIT Division of Health Sciences and Technology Dokmeci, Mehmet Remzi Khademhosseini, Ali Background Current Ebola virus (EBOV) detection methods are costly and impractical for epidemic scenarios. Different immune-based assays have been reported for the detection and quantification of Ebola virus (EBOV) proteins. In particular, several monoclonal antibodies (mAbs) have been described that bind the capsid glycoprotein (GP) of EBOV GP. However, the currently available platforms for the design and production of full-length mAbs are cumbersome and costly. The use of antibody fragments, rather than full-length antibodies, might represent a cost-effective alternative for the development of diagnostic and possibly even therapeutic alternatives for EBOV. Methods/Principal Findings We report the design and expression of three recombinant anti-GP mAb fragments in Escherichia coli cultures. These fragments contained the heavy and light variable portions of the three well-studied anti-GP full-length mAbs 13C6, 13F6, and KZ52, and are consequently named scFv-13C6, scFv-13F6, and Fab-KZ52, respectively. All three fragments exhibited specific anti-GP binding activity in ELISA experiments comparable to that of full-length anti-GP antibodies (i.e., the same order of magnitude) and they are easily and economically produced in bacterial cultures. Conclusion/Significance Antibody fragments might represent a useful, effective, and low cost alternative to full-length antibodies in Ebola related capture and diagnostics applications. National Science Foundation (U.S.) (EFRI-1240443) IMMODGEL 602694 National Institutes of Health (U.S.) (EB012597) National Institutes of Health (U.S.) (AR057837) National Institutes of Health (U.S.) (DE021468) National Institutes of Health (U.S.) (HL099073) National Institutes of Health (U.S.) (AI105024) National Institutes of Health (U.S.) (AR063745) 2015-11-05T18:29:12Z 2015-11-05T18:29:12Z 2015-10 2015-03 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/99735 Rodriguez-Martinez, Luis Mario, Alan Roberto Marquez-Ipina, Felipe Lopez-Pacheco, Roberto Perez-Chavarria, Juan Carlos Gonzalez-Vazquez, Everardo Gonzalez-Gonzalez, Grissel Trujillo-de Santiago, et al. “Antibody Derived Peptides for Detection of Ebola Virus Glycoprotein.” Edited by Gourapura J Renukaradhya. PLoS ONE 10, no. 10 (October 21, 2015): e0135859. en_US http://dx.doi.org/10.1371/journal.pone.0135859 PLOS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/4.0/ application/pdf Public Library of Science Public Library of Science |
| spellingShingle | Rodriguez-Martinez, Luis Mario Marquez-Ipina, Alan Roberto Lopez-Pacheco, Felipe Perez-Chavarria, Roberto Gonzalez-Vazquez, Juan Carlos Gonzalez-Gonzalez, Everardo Trujillo-de Santiago, Grissel Ponce-Ponce de Leon, Cesar Alejandro Zhang, Yu Shrike Dokmeci, Mehmet Remzi Khademhosseini, Ali Alvarez, Mario Moises Antibody Derived Peptides for Detection of Ebola Virus Glycoprotein |
| title | Antibody Derived Peptides for Detection of Ebola Virus Glycoprotein |
| title_full | Antibody Derived Peptides for Detection of Ebola Virus Glycoprotein |
| title_fullStr | Antibody Derived Peptides for Detection of Ebola Virus Glycoprotein |
| title_full_unstemmed | Antibody Derived Peptides for Detection of Ebola Virus Glycoprotein |
| title_short | Antibody Derived Peptides for Detection of Ebola Virus Glycoprotein |
| title_sort | antibody derived peptides for detection of ebola virus glycoprotein |
| url | http://hdl.handle.net/1721.1/99735 |
| work_keys_str_mv | AT rodriguezmartinezluismario antibodyderivedpeptidesfordetectionofebolavirusglycoprotein AT marquezipinaalanroberto antibodyderivedpeptidesfordetectionofebolavirusglycoprotein AT lopezpachecofelipe antibodyderivedpeptidesfordetectionofebolavirusglycoprotein AT perezchavarriaroberto antibodyderivedpeptidesfordetectionofebolavirusglycoprotein AT gonzalezvazquezjuancarlos antibodyderivedpeptidesfordetectionofebolavirusglycoprotein AT gonzalezgonzalezeverardo antibodyderivedpeptidesfordetectionofebolavirusglycoprotein AT trujillodesantiagogrissel antibodyderivedpeptidesfordetectionofebolavirusglycoprotein AT ponceponcedeleoncesaralejandro antibodyderivedpeptidesfordetectionofebolavirusglycoprotein AT zhangyushrike antibodyderivedpeptidesfordetectionofebolavirusglycoprotein AT dokmecimehmetremzi antibodyderivedpeptidesfordetectionofebolavirusglycoprotein AT khademhosseiniali antibodyderivedpeptidesfordetectionofebolavirusglycoprotein AT alvarezmariomoises antibodyderivedpeptidesfordetectionofebolavirusglycoprotein |