Decellularization of fish skin for tissue engineering applications
Collagen is a popular biomaterial in tissue engineering due to its natural abundance and excellent biocompatibility. However, most commercial collagen comes from porcine and bovine sources, which have a risk of transmissible diseases and are unsuitable for patients with religious restrictions. Fish-...
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| Format: | Thesis |
| Language: | English |
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2019
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| Online Access: | https://hdl.handle.net/10356/100060 http://hdl.handle.net/10220/49976 |
| _version_ | 1811691837808705536 |
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| author | Lau, Chau Sang |
| author2 | Teoh Swee Hin |
| author_facet | Teoh Swee Hin Lau, Chau Sang |
| author_sort | Lau, Chau Sang |
| collection | NTU |
| description | Collagen is a popular biomaterial in tissue engineering due to its natural abundance and excellent biocompatibility. However, most commercial collagen comes from porcine and bovine sources, which have a risk of transmissible diseases and are unsuitable for patients with religious restrictions. Fish-derived collagen scaffolds are gaining attention due to their low immunological risk and the abundance of collagen in fish waste such as skin and scales. Among the various fish types, Nile Tilapia (Oreochromis niloticus), a tropical freshwater fish native to Africa, is lauded as a promising source of collagen due to its rapid growth rate and high protein content. Tilapia collagen scaffolds can be formed by reconstituting collagen extracted from tilapia skin, or by removing cells from tilapia skin in a process known as decellularization. While reconstituted tilapia collagen had been studied in tissue engineering, there was very little information on the decellularization of tilapia tissues. This revealed a great potential and novelty for decellularized tilapia skin to be used as a tissue engineering scaffold and to address the growing demand for tissue constructs. In this thesis, we demonstrated that (1) tilapia skin could be decellularized using appropriate treatments to yield an acellular scaffold, (2) the decellularized tilapia skin (DTS) possessed favourable physical and biological properties, and (3) the DTS was suitable as a scaffold in tissue regeneration. The findings in this thesis and subsequent works would help to meet the increasing need for tissue constructs from patients who are unable to take mammalian products, and transform fish waste into useful and valuable biomaterials. |
| first_indexed | 2024-10-01T06:26:14Z |
| format | Thesis |
| id | ntu-10356/100060 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:26:14Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/1000602020-10-28T08:40:48Z Decellularization of fish skin for tissue engineering applications Lau, Chau Sang Teoh Swee Hin School of Chemical and Biomedical Engineering Engineering::Bioengineering Science::Medicine::Tissue engineering Collagen is a popular biomaterial in tissue engineering due to its natural abundance and excellent biocompatibility. However, most commercial collagen comes from porcine and bovine sources, which have a risk of transmissible diseases and are unsuitable for patients with religious restrictions. Fish-derived collagen scaffolds are gaining attention due to their low immunological risk and the abundance of collagen in fish waste such as skin and scales. Among the various fish types, Nile Tilapia (Oreochromis niloticus), a tropical freshwater fish native to Africa, is lauded as a promising source of collagen due to its rapid growth rate and high protein content. Tilapia collagen scaffolds can be formed by reconstituting collagen extracted from tilapia skin, or by removing cells from tilapia skin in a process known as decellularization. While reconstituted tilapia collagen had been studied in tissue engineering, there was very little information on the decellularization of tilapia tissues. This revealed a great potential and novelty for decellularized tilapia skin to be used as a tissue engineering scaffold and to address the growing demand for tissue constructs. In this thesis, we demonstrated that (1) tilapia skin could be decellularized using appropriate treatments to yield an acellular scaffold, (2) the decellularized tilapia skin (DTS) possessed favourable physical and biological properties, and (3) the DTS was suitable as a scaffold in tissue regeneration. The findings in this thesis and subsequent works would help to meet the increasing need for tissue constructs from patients who are unable to take mammalian products, and transform fish waste into useful and valuable biomaterials. Doctor of Philosophy 2019-09-20T08:09:06Z 2019-12-06T20:15:57Z 2019-09-20T08:09:06Z 2019-12-06T20:15:57Z 2019 Thesis Lau, C. S. (2019). Decellularization of fish skin for tissue engineering applications. Doctoral thesis, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/100060 http://hdl.handle.net/10220/49976 10.32657/10356/100060 en 229 p. application/pdf |
| spellingShingle | Engineering::Bioengineering Science::Medicine::Tissue engineering Lau, Chau Sang Decellularization of fish skin for tissue engineering applications |
| title | Decellularization of fish skin for tissue engineering applications |
| title_full | Decellularization of fish skin for tissue engineering applications |
| title_fullStr | Decellularization of fish skin for tissue engineering applications |
| title_full_unstemmed | Decellularization of fish skin for tissue engineering applications |
| title_short | Decellularization of fish skin for tissue engineering applications |
| title_sort | decellularization of fish skin for tissue engineering applications |
| topic | Engineering::Bioengineering Science::Medicine::Tissue engineering |
| url | https://hdl.handle.net/10356/100060 http://hdl.handle.net/10220/49976 |
| work_keys_str_mv | AT lauchausang decellularizationoffishskinfortissueengineeringapplications |