Biofilm growth and near-infrared radiation-driven photosynthesis of the chlorophyll d-containing cyanobacterium acaryochloris marina
The cyanobacterium Acaryochloris marina is the only known phototroph harboring chlorophyll (Chl) d. It is easy to cultivate it in a planktonic growth mode, and A. marina cultures have been subject to detailed biochemical and biophysical characterization. In natural situations, A. marina is mainly fo...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2013
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| Online Access: | https://hdl.handle.net/10356/100269 http://hdl.handle.net/10220/10965 |
| _version_ | 1826118144574357504 |
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| author | Behrendt, Lars Schrameyer, Verena Qvortrup, Klaus Lundin, Luisa Kühl, Michael Sørensen, Søren J. Larkum, Anthony W. D. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Behrendt, Lars Schrameyer, Verena Qvortrup, Klaus Lundin, Luisa Kühl, Michael Sørensen, Søren J. Larkum, Anthony W. D. |
| author_sort | Behrendt, Lars |
| collection | NTU |
| description | The cyanobacterium Acaryochloris marina is the only known phototroph harboring chlorophyll (Chl) d. It is easy to cultivate it in a planktonic growth mode, and A. marina cultures have been subject to detailed biochemical and biophysical characterization. In natural situations, A. marina is mainly found associated with surfaces, but this growth mode has not been studied yet. Here, we show that the A. marina type strain MBIC11017 inoculated into alginate beads forms dense biofilm-like cell clusters, as in natural A. marina biofilms, characterized by strong O2 concentration gradients that change with irradiance. Biofilm growth under both visible radiation (VIS, 400 to 700 nm) and near-infrared radiation (NIR, ∼700 to 730 nm) yielded maximal cell-specific growth rates of 0.38 per day and 0.64 per day, respectively. The population doubling times were 1.09 and 1.82 days for NIR and visible light, respectively. The photosynthesis versus irradiance curves showed saturation at a photon irradiance of Ek (saturating irradiance) >250 μmol photons m−2 s−1 for blue light but no clear saturation at 365 μmol photons m−2 s−1 for NIR. The maximal gross photosynthesis rates in the aggregates were ∼1,272 μmol O2 mg Chl d−1 h−1 (NIR) and ∼1,128 μmol O2 mg Chl d−1 h−1 (VIS). The photosynthetic efficiency (α) values were higher in NIR-irradiated cells [(268 ± 0.29) × 10−6 m2 mg Chl d−1 (mean ± standard deviation)] than under blue light [(231 ± 0.22) × 10−6 m2 mg Chl d−1]. A. marina is well adapted to a biofilm growth mode under both visible and NIR irradiance and under O2 conditions ranging from anoxia to hyperoxia, explaining its presence in natural niches with similar environmental conditions. |
| first_indexed | 2024-10-01T04:38:55Z |
| format | Journal Article |
| id | ntu-10356/100269 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:38:55Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/1002692022-02-16T16:31:22Z Biofilm growth and near-infrared radiation-driven photosynthesis of the chlorophyll d-containing cyanobacterium acaryochloris marina Behrendt, Lars Schrameyer, Verena Qvortrup, Klaus Lundin, Luisa Kühl, Michael Sørensen, Søren J. Larkum, Anthony W. D. School of Biological Sciences DRNTU::Science::Biological sciences The cyanobacterium Acaryochloris marina is the only known phototroph harboring chlorophyll (Chl) d. It is easy to cultivate it in a planktonic growth mode, and A. marina cultures have been subject to detailed biochemical and biophysical characterization. In natural situations, A. marina is mainly found associated with surfaces, but this growth mode has not been studied yet. Here, we show that the A. marina type strain MBIC11017 inoculated into alginate beads forms dense biofilm-like cell clusters, as in natural A. marina biofilms, characterized by strong O2 concentration gradients that change with irradiance. Biofilm growth under both visible radiation (VIS, 400 to 700 nm) and near-infrared radiation (NIR, ∼700 to 730 nm) yielded maximal cell-specific growth rates of 0.38 per day and 0.64 per day, respectively. The population doubling times were 1.09 and 1.82 days for NIR and visible light, respectively. The photosynthesis versus irradiance curves showed saturation at a photon irradiance of Ek (saturating irradiance) >250 μmol photons m−2 s−1 for blue light but no clear saturation at 365 μmol photons m−2 s−1 for NIR. The maximal gross photosynthesis rates in the aggregates were ∼1,272 μmol O2 mg Chl d−1 h−1 (NIR) and ∼1,128 μmol O2 mg Chl d−1 h−1 (VIS). The photosynthetic efficiency (α) values were higher in NIR-irradiated cells [(268 ± 0.29) × 10−6 m2 mg Chl d−1 (mean ± standard deviation)] than under blue light [(231 ± 0.22) × 10−6 m2 mg Chl d−1]. A. marina is well adapted to a biofilm growth mode under both visible and NIR irradiance and under O2 conditions ranging from anoxia to hyperoxia, explaining its presence in natural niches with similar environmental conditions. 2013-07-05T02:14:04Z 2019-12-06T20:19:24Z 2013-07-05T02:14:04Z 2019-12-06T20:19:24Z 2012 2012 Journal Article Behrendt, L., Schrameyer, V., Qvortrup, K., Lundin, L., Sørensen, S. J., Larkum, A. W. D., et al. (2012). Biofilm Growth and Near-Infrared Radiation-Driven Photosynthesis of the Chlorophyll d-Containing Cyanobacterium Acaryochloris marina. Applied and Environmental Microbiology, 78(11), 3896-3904. 0099-2240 https://hdl.handle.net/10356/100269 http://hdl.handle.net/10220/10965 10.1128/AEM.00397-12 22467501 en Applied and environmental microbiology © 2012 American Society for Microbiology. |
| spellingShingle | DRNTU::Science::Biological sciences Behrendt, Lars Schrameyer, Verena Qvortrup, Klaus Lundin, Luisa Kühl, Michael Sørensen, Søren J. Larkum, Anthony W. D. Biofilm growth and near-infrared radiation-driven photosynthesis of the chlorophyll d-containing cyanobacterium acaryochloris marina |
| title | Biofilm growth and near-infrared radiation-driven photosynthesis of the chlorophyll d-containing cyanobacterium acaryochloris marina |
| title_full | Biofilm growth and near-infrared radiation-driven photosynthesis of the chlorophyll d-containing cyanobacterium acaryochloris marina |
| title_fullStr | Biofilm growth and near-infrared radiation-driven photosynthesis of the chlorophyll d-containing cyanobacterium acaryochloris marina |
| title_full_unstemmed | Biofilm growth and near-infrared radiation-driven photosynthesis of the chlorophyll d-containing cyanobacterium acaryochloris marina |
| title_short | Biofilm growth and near-infrared radiation-driven photosynthesis of the chlorophyll d-containing cyanobacterium acaryochloris marina |
| title_sort | biofilm growth and near infrared radiation driven photosynthesis of the chlorophyll d containing cyanobacterium acaryochloris marina |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/100269 http://hdl.handle.net/10220/10965 |
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