Non-linear optical microscopy sheds light on cardiovascular disease
Many cardiac diseases have been associated with increased fibrosis and changes in the organization of fibrillar collagen. The degree of fibrosis is routinely analyzed with invasive histological and immunohistochemical methods, giving a limited and qualitative understanding of the tissue's morph...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2014
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| Online Access: | https://hdl.handle.net/10356/100293 http://hdl.handle.net/10220/18448 |
| _version_ | 1811686099706183680 |
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| author | Lyon, Alexander R. Ferenczi, Mike A. Sikkel, Markus B. Caorsi, Valentina Toepfer, Christopher MacLeod, Ken |
| author2 | Talkachova, Alena |
| author_facet | Talkachova, Alena Lyon, Alexander R. Ferenczi, Mike A. Sikkel, Markus B. Caorsi, Valentina Toepfer, Christopher MacLeod, Ken |
| author_sort | Lyon, Alexander R. |
| collection | NTU |
| description | Many cardiac diseases have been associated with increased fibrosis and changes in the organization of fibrillar collagen. The degree of fibrosis is routinely analyzed with invasive histological and immunohistochemical methods, giving a limited and qualitative understanding of the tissue's morphological adaptation to disease. Our aim is to quantitatively evaluate the increase in fibrosis by three-dimensional imaging of the collagen network in the myocardium using the non-linear optical microscopy techniques Two-Photon Excitation microscopy (TPE) and Second Harmonic signal Generation (SHG). No sample staining is needed because numerous endogenous fluorophores are excited by a two-photon mechanism and highly non-centrosymmetric structures such as collagen generate strong second harmonic signals. We propose for the first time a 3D quantitative analysis to carefully evaluate the increased fibrosis in tissue from a rat model of heart failure post myocardial infarction. We show how to measure changes in fibrosis from the backward SHG (BSHG) alone, as only backward-propagating SHG is accessible for true in vivo applications. A 5-fold increase in collagen I fibrosis is detected in the remote surviving myocardium measured 20 weeks after infarction. The spatial distribution is also shown to change markedly, providing insight into the morphology of disease progression. |
| first_indexed | 2024-10-01T04:55:02Z |
| format | Journal Article |
| id | ntu-10356/100293 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:55:02Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/1002932022-02-16T16:31:06Z Non-linear optical microscopy sheds light on cardiovascular disease Lyon, Alexander R. Ferenczi, Mike A. Sikkel, Markus B. Caorsi, Valentina Toepfer, Christopher MacLeod, Ken Talkachova, Alena Lee Kong Chian School of Medicine (LKCMedicine) Medicine Many cardiac diseases have been associated with increased fibrosis and changes in the organization of fibrillar collagen. The degree of fibrosis is routinely analyzed with invasive histological and immunohistochemical methods, giving a limited and qualitative understanding of the tissue's morphological adaptation to disease. Our aim is to quantitatively evaluate the increase in fibrosis by three-dimensional imaging of the collagen network in the myocardium using the non-linear optical microscopy techniques Two-Photon Excitation microscopy (TPE) and Second Harmonic signal Generation (SHG). No sample staining is needed because numerous endogenous fluorophores are excited by a two-photon mechanism and highly non-centrosymmetric structures such as collagen generate strong second harmonic signals. We propose for the first time a 3D quantitative analysis to carefully evaluate the increased fibrosis in tissue from a rat model of heart failure post myocardial infarction. We show how to measure changes in fibrosis from the backward SHG (BSHG) alone, as only backward-propagating SHG is accessible for true in vivo applications. A 5-fold increase in collagen I fibrosis is detected in the remote surviving myocardium measured 20 weeks after infarction. The spatial distribution is also shown to change markedly, providing insight into the morphology of disease progression. Published version 2014-01-13T02:08:07Z 2019-12-06T20:19:52Z 2014-01-13T02:08:07Z 2019-12-06T20:19:52Z 2013 2013 Journal Article Caorsi, V., Toepfer, C., Sikkel, M. B., Lyon, A. R., MacLeod, K., & Ferenczi, M. A. (2013). Non-Linear Optical Microscopy Sheds Light on Cardiovascular Disease. PLoS ONE, 8(2), e56136-. 1932-6203 https://hdl.handle.net/10356/100293 http://hdl.handle.net/10220/18448 10.1371/journal.pone.0056136 23409139 en PLoS ONE © 2013 The Authors. This paper was published in PLoS ONE and is made available as an electronic reprint (preprint) with permission of the authors. The paper can be found at the following official DOI: [http://dx.doi.org/10.1371/journal.pone.0056136]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. application/pdf |
| spellingShingle | Medicine Lyon, Alexander R. Ferenczi, Mike A. Sikkel, Markus B. Caorsi, Valentina Toepfer, Christopher MacLeod, Ken Non-linear optical microscopy sheds light on cardiovascular disease |
| title | Non-linear optical microscopy sheds light on cardiovascular disease |
| title_full | Non-linear optical microscopy sheds light on cardiovascular disease |
| title_fullStr | Non-linear optical microscopy sheds light on cardiovascular disease |
| title_full_unstemmed | Non-linear optical microscopy sheds light on cardiovascular disease |
| title_short | Non-linear optical microscopy sheds light on cardiovascular disease |
| title_sort | non linear optical microscopy sheds light on cardiovascular disease |
| topic | Medicine |
| url | https://hdl.handle.net/10356/100293 http://hdl.handle.net/10220/18448 |
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