Fe(III) reduction and U(VI) immobilization by Paenibacillus sp. strain 300A, isolated from Hanford 300A subsurface sediments
A facultative iron-reducing [Fe(III)-reducing] Paenibacillus sp. strain was isolated from Hanford 300A subsurface sediment biofilms that was capable of reducing soluble Fe(III) complexes [Fe(III)-nitrilotriacetic acid and Fe(III)-citrate] but unable to reduce poorly crystalline ferrihydrite (Fh). Ho...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2013
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| Online Access: | https://hdl.handle.net/10356/100340 http://hdl.handle.net/10220/10967 |
| _version_ | 1826111004918939648 |
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| author | McLean, Jeffrey S. Fredrickson, Jim K. Ahmed, Bulbul Cao, Bin Ica, Tuba Dohnalkova, Alice Istanbullu, Ozlem Paksoy, Akin Beyenal, Haluk |
| author_facet | McLean, Jeffrey S. Fredrickson, Jim K. Ahmed, Bulbul Cao, Bin Ica, Tuba Dohnalkova, Alice Istanbullu, Ozlem Paksoy, Akin Beyenal, Haluk |
| author_sort | McLean, Jeffrey S. |
| collection | NTU |
| description | A facultative iron-reducing [Fe(III)-reducing] Paenibacillus sp. strain was isolated from Hanford 300A subsurface sediment biofilms that was capable of reducing soluble Fe(III) complexes [Fe(III)-nitrilotriacetic acid and Fe(III)-citrate] but unable to reduce poorly crystalline ferrihydrite (Fh). However, Paenibacillus sp. 300A was capable of reducing Fh in the presence of low concentrations (2 μM) of either of the electron transfer mediators (ETMs) flavin mononucleotide (FMN) or anthraquinone-2,6-disulfonate (AQDS). Maximum initial Fh reduction rates were observed at catalytic concentrations (<10 μM) of either FMN or AQDS. Higher FMN concentrations inhibited Fh reduction, while increased AQDS concentrations did not. We also found that Paenibacillus sp. 300A could reduce Fh in the presence of natural ETMs from Hanford 300A subsurface sediments. In the absence of ETMs, Paenibacillus sp. 300A was capable of immobilizing U(VI) through both reduction and adsorption. The relative contributions of adsorption and microbial reduction to U(VI) removal from the aqueous phase were ∼7:3 in PIPES [piperazine-N,N′-bis(2-ethanesulfonic acid)] and ∼1:4 in bicarbonate buffer. Our study demonstrated that Paenibacillus sp. 300A catalyzes Fe(III) reduction and U(VI) immobilization and that these reactions benefit from externally added or naturally existing ETMs in 300A subsurface sediments. |
| first_indexed | 2024-10-01T02:43:30Z |
| format | Journal Article |
| id | ntu-10356/100340 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:43:30Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/1003402022-02-16T16:31:20Z Fe(III) reduction and U(VI) immobilization by Paenibacillus sp. strain 300A, isolated from Hanford 300A subsurface sediments McLean, Jeffrey S. Fredrickson, Jim K. Ahmed, Bulbul Cao, Bin Ica, Tuba Dohnalkova, Alice Istanbullu, Ozlem Paksoy, Akin Beyenal, Haluk A facultative iron-reducing [Fe(III)-reducing] Paenibacillus sp. strain was isolated from Hanford 300A subsurface sediment biofilms that was capable of reducing soluble Fe(III) complexes [Fe(III)-nitrilotriacetic acid and Fe(III)-citrate] but unable to reduce poorly crystalline ferrihydrite (Fh). However, Paenibacillus sp. 300A was capable of reducing Fh in the presence of low concentrations (2 μM) of either of the electron transfer mediators (ETMs) flavin mononucleotide (FMN) or anthraquinone-2,6-disulfonate (AQDS). Maximum initial Fh reduction rates were observed at catalytic concentrations (<10 μM) of either FMN or AQDS. Higher FMN concentrations inhibited Fh reduction, while increased AQDS concentrations did not. We also found that Paenibacillus sp. 300A could reduce Fh in the presence of natural ETMs from Hanford 300A subsurface sediments. In the absence of ETMs, Paenibacillus sp. 300A was capable of immobilizing U(VI) through both reduction and adsorption. The relative contributions of adsorption and microbial reduction to U(VI) removal from the aqueous phase were ∼7:3 in PIPES [piperazine-N,N′-bis(2-ethanesulfonic acid)] and ∼1:4 in bicarbonate buffer. Our study demonstrated that Paenibacillus sp. 300A catalyzes Fe(III) reduction and U(VI) immobilization and that these reactions benefit from externally added or naturally existing ETMs in 300A subsurface sediments. 2013-07-05T02:21:00Z 2019-12-06T20:20:49Z 2013-07-05T02:21:00Z 2019-12-06T20:20:49Z 2012 2012 Journal Article Ahmed, B., Cao, B., McLean, J. S., Ica, T., Dohnalkova, A., Istanbullu, O., et al. (2012). Fe(III) Reduction and U(VI) Immobilization by Paenibacillus sp. Strain 300A, Isolated from Hanford 300A Subsurface Sediments. Applied and Environmental Microbiology, 78(22), 8001-8009. 0099-2240 https://hdl.handle.net/10356/100340 http://hdl.handle.net/10220/10967 10.1128/AEM.01844-12 22961903 en Applied and environmental microbiology © 2012 American Society for Microbiology. |
| spellingShingle | McLean, Jeffrey S. Fredrickson, Jim K. Ahmed, Bulbul Cao, Bin Ica, Tuba Dohnalkova, Alice Istanbullu, Ozlem Paksoy, Akin Beyenal, Haluk Fe(III) reduction and U(VI) immobilization by Paenibacillus sp. strain 300A, isolated from Hanford 300A subsurface sediments |
| title | Fe(III) reduction and U(VI) immobilization by Paenibacillus sp. strain 300A, isolated from Hanford 300A subsurface sediments |
| title_full | Fe(III) reduction and U(VI) immobilization by Paenibacillus sp. strain 300A, isolated from Hanford 300A subsurface sediments |
| title_fullStr | Fe(III) reduction and U(VI) immobilization by Paenibacillus sp. strain 300A, isolated from Hanford 300A subsurface sediments |
| title_full_unstemmed | Fe(III) reduction and U(VI) immobilization by Paenibacillus sp. strain 300A, isolated from Hanford 300A subsurface sediments |
| title_short | Fe(III) reduction and U(VI) immobilization by Paenibacillus sp. strain 300A, isolated from Hanford 300A subsurface sediments |
| title_sort | fe iii reduction and u vi immobilization by paenibacillus sp strain 300a isolated from hanford 300a subsurface sediments |
| url | https://hdl.handle.net/10356/100340 http://hdl.handle.net/10220/10967 |
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