Highly sensitive electrochemical determination of neutrophil gelatinase-associated lipocalin for acute kidney injury
A label-free electrochemical immunosensor for the detection of neutrophil gelatinase-associated lipocalin (NGAL) is developed by the immobilization of rabbit polygonal lipocalin-2 antibody on gold nanoparticles attached on generation-1polyamidoamine (PAMAM) dendrimer (LA2/AuNPs/PAMAM)-modified gold...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Online Access: | https://hdl.handle.net/10356/100522 http://hdl.handle.net/10220/11053 |
| _version_ | 1826119384060395520 |
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| author | Kannan, Palanisamy Tiong, Ho Yee Kim, Dong-Hwan |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Kannan, Palanisamy Tiong, Ho Yee Kim, Dong-Hwan |
| author_sort | Kannan, Palanisamy |
| collection | NTU |
| description | A label-free electrochemical immunosensor for the detection of neutrophil gelatinase-associated lipocalin (NGAL) is developed by the immobilization of rabbit polygonal lipocalin-2 antibody on gold nanoparticles attached on generation-1polyamidoamine (PAMAM) dendrimer (LA2/AuNPs/PAMAM)-modified gold electrode. The modification procedure was characterized by UV–vis, surface enhanced Raman spectroscopy and field-emission scanning electron microscopy techniques. The detection of NGAL is based on the enhancement of oxidation current on the modified electrodes upon the antigen–antibody interaction. The electrochemical immunosensor exhibited high sensitivity (1 ng mL−1 (280 pM) based on the signal-to-noise ratio 3), wide linear range (50–250 ng mL−1) and long-term stability. The reliability of the developed immunosensor was investigated by the detection of NGAL in both blood serum and urine samples. |
| first_indexed | 2024-10-01T04:59:25Z |
| format | Journal Article |
| id | ntu-10356/100522 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:59:25Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/1005222020-03-07T11:35:32Z Highly sensitive electrochemical determination of neutrophil gelatinase-associated lipocalin for acute kidney injury Kannan, Palanisamy Tiong, Ho Yee Kim, Dong-Hwan School of Chemical and Biomedical Engineering A label-free electrochemical immunosensor for the detection of neutrophil gelatinase-associated lipocalin (NGAL) is developed by the immobilization of rabbit polygonal lipocalin-2 antibody on gold nanoparticles attached on generation-1polyamidoamine (PAMAM) dendrimer (LA2/AuNPs/PAMAM)-modified gold electrode. The modification procedure was characterized by UV–vis, surface enhanced Raman spectroscopy and field-emission scanning electron microscopy techniques. The detection of NGAL is based on the enhancement of oxidation current on the modified electrodes upon the antigen–antibody interaction. The electrochemical immunosensor exhibited high sensitivity (1 ng mL−1 (280 pM) based on the signal-to-noise ratio 3), wide linear range (50–250 ng mL−1) and long-term stability. The reliability of the developed immunosensor was investigated by the detection of NGAL in both blood serum and urine samples. 2013-07-09T04:00:58Z 2019-12-06T20:24:00Z 2013-07-09T04:00:58Z 2019-12-06T20:24:00Z 2011 2011 Journal Article Kannan, P., Tiong, H. Y., Kim, D.-H. (2001). Highly sensitive electrochemical determination of neutrophil gelatinase-associated lipocalin for acute kidney injury. Biosensors and Bioelectronics, 31(1), 32-36. https://hdl.handle.net/10356/100522 http://hdl.handle.net/10220/11053 10.1016/j.bios.2011.09.036 en Biosensors and bioelectronics © 2011 Elsevier B.V. |
| spellingShingle | Kannan, Palanisamy Tiong, Ho Yee Kim, Dong-Hwan Highly sensitive electrochemical determination of neutrophil gelatinase-associated lipocalin for acute kidney injury |
| title | Highly sensitive electrochemical determination of neutrophil gelatinase-associated lipocalin for acute kidney injury |
| title_full | Highly sensitive electrochemical determination of neutrophil gelatinase-associated lipocalin for acute kidney injury |
| title_fullStr | Highly sensitive electrochemical determination of neutrophil gelatinase-associated lipocalin for acute kidney injury |
| title_full_unstemmed | Highly sensitive electrochemical determination of neutrophil gelatinase-associated lipocalin for acute kidney injury |
| title_short | Highly sensitive electrochemical determination of neutrophil gelatinase-associated lipocalin for acute kidney injury |
| title_sort | highly sensitive electrochemical determination of neutrophil gelatinase associated lipocalin for acute kidney injury |
| url | https://hdl.handle.net/10356/100522 http://hdl.handle.net/10220/11053 |
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