Engineering bioconjugated gold nanospheres and gold nanorods as label-free plasmon scattering probes for ultrasensitive multiplex dark-field imaging of cancer cells
In this paper, we proposed the use of gold nanoparticles as plasmon scattering probes for dark-field multiplex imaging of live cancer cells. By carefully engineering the surfaces, aqueous dispersions of anti-EGFR antibody-conjugated gold nanospheres and gold nanorods are prepared. We demonstrated th...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2014
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| Online Access: | https://hdl.handle.net/10356/100529 http://hdl.handle.net/10220/24122 |
| _version_ | 1826124132768546816 |
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| author | Gong, Tianxun Olivo, Malini Dinish, U. S. Goh, Douglas Kong, Kien Voon Yong, Ken-Tye |
| author2 | School of Electrical and Electronic Engineering |
| author_facet | School of Electrical and Electronic Engineering Gong, Tianxun Olivo, Malini Dinish, U. S. Goh, Douglas Kong, Kien Voon Yong, Ken-Tye |
| author_sort | Gong, Tianxun |
| collection | NTU |
| description | In this paper, we proposed the use of gold nanoparticles as plasmon scattering probes for dark-field multiplex imaging of live cancer cells. By carefully engineering the surfaces, aqueous dispersions of anti-EGFR antibody-conjugated gold nanospheres and gold nanorods are prepared. We demonstrated the receptor-mediated delivery of antibody conjugated gold nanospheres and gold nanorods into EGFR receptor-positive oral squamous cell carcinoma cell line by using dark-field microscopy technique. Our result suggests that gold nanospheres and gold nanorods formulations could provide up to 7 types of plasmon scattering probes based on their tunability range with a 100 nm spectral separation in absorption bands. |
| first_indexed | 2024-10-01T06:15:40Z |
| format | Journal Article |
| id | ntu-10356/100529 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:15:40Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/1005292020-03-07T14:02:40Z Engineering bioconjugated gold nanospheres and gold nanorods as label-free plasmon scattering probes for ultrasensitive multiplex dark-field imaging of cancer cells Gong, Tianxun Olivo, Malini Dinish, U. S. Goh, Douglas Kong, Kien Voon Yong, Ken-Tye School of Electrical and Electronic Engineering DRNTU::Science::Medicine::Biomedical engineering In this paper, we proposed the use of gold nanoparticles as plasmon scattering probes for dark-field multiplex imaging of live cancer cells. By carefully engineering the surfaces, aqueous dispersions of anti-EGFR antibody-conjugated gold nanospheres and gold nanorods are prepared. We demonstrated the receptor-mediated delivery of antibody conjugated gold nanospheres and gold nanorods into EGFR receptor-positive oral squamous cell carcinoma cell line by using dark-field microscopy technique. Our result suggests that gold nanospheres and gold nanorods formulations could provide up to 7 types of plasmon scattering probes based on their tunability range with a 100 nm spectral separation in absorption bands. 2014-10-24T05:55:20Z 2019-12-06T20:24:04Z 2014-10-24T05:55:20Z 2019-12-06T20:24:04Z 2013 2013 Journal Article Gong, T., Olivo, M., Dinish, U. S., Goh, D., Kong, K. V., & Yong, K.-T. (2013). Engineering bioconjugated gold nanospheres and gold nanorods as label-free plasmon scattering probes for ultrasensitive multiplex dark-field imaging of cancer cells. Journal of biomedical nanotechnology, 9(6), 985-991. 1550-7033 https://hdl.handle.net/10356/100529 http://hdl.handle.net/10220/24122 10.1166/jbn.2013.1603 en Journal of biomedical nanotechnology © 2013 American Scientific Publishers. |
| spellingShingle | DRNTU::Science::Medicine::Biomedical engineering Gong, Tianxun Olivo, Malini Dinish, U. S. Goh, Douglas Kong, Kien Voon Yong, Ken-Tye Engineering bioconjugated gold nanospheres and gold nanorods as label-free plasmon scattering probes for ultrasensitive multiplex dark-field imaging of cancer cells |
| title | Engineering bioconjugated gold nanospheres and gold nanorods as label-free plasmon scattering probes for ultrasensitive multiplex dark-field imaging of cancer cells |
| title_full | Engineering bioconjugated gold nanospheres and gold nanorods as label-free plasmon scattering probes for ultrasensitive multiplex dark-field imaging of cancer cells |
| title_fullStr | Engineering bioconjugated gold nanospheres and gold nanorods as label-free plasmon scattering probes for ultrasensitive multiplex dark-field imaging of cancer cells |
| title_full_unstemmed | Engineering bioconjugated gold nanospheres and gold nanorods as label-free plasmon scattering probes for ultrasensitive multiplex dark-field imaging of cancer cells |
| title_short | Engineering bioconjugated gold nanospheres and gold nanorods as label-free plasmon scattering probes for ultrasensitive multiplex dark-field imaging of cancer cells |
| title_sort | engineering bioconjugated gold nanospheres and gold nanorods as label free plasmon scattering probes for ultrasensitive multiplex dark field imaging of cancer cells |
| topic | DRNTU::Science::Medicine::Biomedical engineering |
| url | https://hdl.handle.net/10356/100529 http://hdl.handle.net/10220/24122 |
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