Water-Soluble pillararene-functionalized graphene oxide for in vitro raman and fluorescence dual-mode imaging
This study provides a successful preparation of biocompatible hybrid materials (1-GO and 2-GO) by the integration of graphene oxide (GO) with water-soluble pillararenes (bolaamphiphile 1 and tadpolelike amphiphile 2) for dual-mode Raman and fluorescence bioimaging in vitro. The investigations show t...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2014
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| Online Access: | https://hdl.handle.net/10356/100962 http://hdl.handle.net/10220/19691 |
| _version_ | 1826127237568528384 |
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| author | Zhao, Yanli Zhang, Huacheng Ma, Xing Nguyen, Kim Truc Zeng, Yongfei Tai, Shuhui |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Zhao, Yanli Zhang, Huacheng Ma, Xing Nguyen, Kim Truc Zeng, Yongfei Tai, Shuhui |
| author_sort | Zhao, Yanli |
| collection | NTU |
| description | This study provides a successful preparation of biocompatible hybrid materials (1-GO and 2-GO) by the integration of graphene oxide (GO) with water-soluble pillararenes (bolaamphiphile 1 and tadpolelike amphiphile 2) for dual-mode Raman and fluorescence bioimaging in vitro. The investigations show that pillararenes 1 and 2 were loaded onto the surface of GO through strong hydrogen-bonding interactions. Aqueous suspensions of 1-GO and 2-GO are stable and can be kept for a long time. After confirming their good biocompatibility by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the 1-GO and 2-GO hybrids were endocytosed by HeLa cells for in vitro Raman imaging. It was found that 1-GO presents better Raman imaging than 2-GO. When a fluorescent guest molecule, bipyridinium derivative 3, was added into the suspensions of the hybrids, the suspensions of 1-GO and 2-GO were as stable as the original. The suspensions of the inclusion complexes (1-GO[DOT OPERATOR]3 and 2-GO[DOT OPERATOR]3) formed from 1-GO and 2-GO with 3 can also be endocytosed by HeLa cells to enable in vitro fluorescence imaging to be performed. It was found that 1-GO[DOT OPERATOR]3 performs better than 2-GO[DOT OPERATOR]3. The current research has determined the capacities of pillararene-modified GO for combined bioimaging, which paves the way for using these biocompatible materials towards dual-mode diagnostics. |
| first_indexed | 2024-10-01T07:05:31Z |
| format | Journal Article |
| id | ntu-10356/100962 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:05:31Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/1009622020-06-01T10:26:49Z Water-Soluble pillararene-functionalized graphene oxide for in vitro raman and fluorescence dual-mode imaging Zhao, Yanli Zhang, Huacheng Ma, Xing Nguyen, Kim Truc Zeng, Yongfei Tai, Shuhui School of Materials Science & Engineering School of Physical and Mathematical Sciences DRNTU::Engineering::Materials::Biomaterials This study provides a successful preparation of biocompatible hybrid materials (1-GO and 2-GO) by the integration of graphene oxide (GO) with water-soluble pillararenes (bolaamphiphile 1 and tadpolelike amphiphile 2) for dual-mode Raman and fluorescence bioimaging in vitro. The investigations show that pillararenes 1 and 2 were loaded onto the surface of GO through strong hydrogen-bonding interactions. Aqueous suspensions of 1-GO and 2-GO are stable and can be kept for a long time. After confirming their good biocompatibility by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the 1-GO and 2-GO hybrids were endocytosed by HeLa cells for in vitro Raman imaging. It was found that 1-GO presents better Raman imaging than 2-GO. When a fluorescent guest molecule, bipyridinium derivative 3, was added into the suspensions of the hybrids, the suspensions of 1-GO and 2-GO were as stable as the original. The suspensions of the inclusion complexes (1-GO[DOT OPERATOR]3 and 2-GO[DOT OPERATOR]3) formed from 1-GO and 2-GO with 3 can also be endocytosed by HeLa cells to enable in vitro fluorescence imaging to be performed. It was found that 1-GO[DOT OPERATOR]3 performs better than 2-GO[DOT OPERATOR]3. The current research has determined the capacities of pillararene-modified GO for combined bioimaging, which paves the way for using these biocompatible materials towards dual-mode diagnostics. 2014-06-12T03:42:28Z 2019-12-06T20:31:29Z 2014-06-12T03:42:28Z 2019-12-06T20:31:29Z 2014 2014 Journal Article Zhang, H., Ma, X., Nguyen, K. T., Zeng, Y., Tai, S., & Zhao, Y. (2014). Water-Soluble Pillararene-Functionalized Graphene Oxide for In Vitro Raman and Fluorescence Dual-Mode Imaging. ChemPlusChem, 79(3), 462-469. 2192-6506 https://hdl.handle.net/10356/100962 http://hdl.handle.net/10220/19691 10.1002/cplu.201300408 en ChemPlusChem © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| spellingShingle | DRNTU::Engineering::Materials::Biomaterials Zhao, Yanli Zhang, Huacheng Ma, Xing Nguyen, Kim Truc Zeng, Yongfei Tai, Shuhui Water-Soluble pillararene-functionalized graphene oxide for in vitro raman and fluorescence dual-mode imaging |
| title | Water-Soluble pillararene-functionalized graphene oxide for in vitro raman and fluorescence dual-mode imaging |
| title_full | Water-Soluble pillararene-functionalized graphene oxide for in vitro raman and fluorescence dual-mode imaging |
| title_fullStr | Water-Soluble pillararene-functionalized graphene oxide for in vitro raman and fluorescence dual-mode imaging |
| title_full_unstemmed | Water-Soluble pillararene-functionalized graphene oxide for in vitro raman and fluorescence dual-mode imaging |
| title_short | Water-Soluble pillararene-functionalized graphene oxide for in vitro raman and fluorescence dual-mode imaging |
| title_sort | water soluble pillararene functionalized graphene oxide for in vitro raman and fluorescence dual mode imaging |
| topic | DRNTU::Engineering::Materials::Biomaterials |
| url | https://hdl.handle.net/10356/100962 http://hdl.handle.net/10220/19691 |
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