The basis for rootstock resilient to Capnodis species : screening for genes encoding δ-endotoxins from Bacillus thuringiensis
BACKGROUND Conventional methods often fail to control the flatheaded borers Capnodis spp., major pests of stone fruit trees; the larvae are protected from insecticides and predation because they feed deep in the roots. A potential solution is transgenic trees producing in their roots toxic comp...
| Main Authors: | , , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2014
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/101569 http://hdl.handle.net/10220/19728 |
| _version_ | 1811683371756027904 |
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| author | Gindin, Galina Mendel, Zvi Levitin, Bella Kumar, Pradeep Kuznetsova, Tatiana Levi, Tal Shahi, Preeti Khasdan, Vadim Weinthal, Dan Einav, Monica Kushmaro, Ariel Protasov, Alex Zaritsky, Arieh Ben-Dov, Eitan |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Gindin, Galina Mendel, Zvi Levitin, Bella Kumar, Pradeep Kuznetsova, Tatiana Levi, Tal Shahi, Preeti Khasdan, Vadim Weinthal, Dan Einav, Monica Kushmaro, Ariel Protasov, Alex Zaritsky, Arieh Ben-Dov, Eitan |
| author_sort | Gindin, Galina |
| collection | NTU |
| description | BACKGROUND
Conventional methods often fail to control the flatheaded borers Capnodis spp., major pests of stone fruit trees; the larvae are protected from insecticides and predation because they feed deep in the roots. A potential solution is transgenic trees producing in their roots toxic compounds such as Cry proteins of Bacillus thuringiensis (Bt).
RESULTS
Toxicities against Capnodis larvae were demonstrated by exploiting a recently designed artificial larval diet and an available collection of field isolated Bt. An isolate of Bt tenebrionis (Btt) from commercial bioinsecticide (Novodor) displayed LC50 and LC95 values of 3.2 and 164 mg g−1, respectively, against neonates of Capnodis tenebrionis, whereas values of the most toxic field isolate K-7 were 1.9 and 25.6 mg g−1 respectively. Weights of surviving larvae after 1 month on diets containing low concentrations of K-7 (0.1–1.0 mg g−1) were lower than on Btt or untreated larvae. K-7 was also toxic against larvae of C. cariosa and C. miliaris and found to harbour genes encoding Cry9Ea-like and Cry23Aa/Cry37Aa binary toxins.
CONCLUSION
Larvae of Capnodis spp. are susceptible to Bt Cry toxins. Expressing cry genes active against these pests thus seems a feasible solution towards production of transgenic rootstock trees resilient to the pest. |
| first_indexed | 2024-10-01T04:11:41Z |
| format | Journal Article |
| id | ntu-10356/101569 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:11:41Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/1015692020-06-01T10:01:35Z The basis for rootstock resilient to Capnodis species : screening for genes encoding δ-endotoxins from Bacillus thuringiensis Gindin, Galina Mendel, Zvi Levitin, Bella Kumar, Pradeep Kuznetsova, Tatiana Levi, Tal Shahi, Preeti Khasdan, Vadim Weinthal, Dan Einav, Monica Kushmaro, Ariel Protasov, Alex Zaritsky, Arieh Ben-Dov, Eitan School of Materials Science & Engineering DRNTU::Science::Biological sciences::Botany DRNTU::Engineering::Materials BACKGROUND Conventional methods often fail to control the flatheaded borers Capnodis spp., major pests of stone fruit trees; the larvae are protected from insecticides and predation because they feed deep in the roots. A potential solution is transgenic trees producing in their roots toxic compounds such as Cry proteins of Bacillus thuringiensis (Bt). RESULTS Toxicities against Capnodis larvae were demonstrated by exploiting a recently designed artificial larval diet and an available collection of field isolated Bt. An isolate of Bt tenebrionis (Btt) from commercial bioinsecticide (Novodor) displayed LC50 and LC95 values of 3.2 and 164 mg g−1, respectively, against neonates of Capnodis tenebrionis, whereas values of the most toxic field isolate K-7 were 1.9 and 25.6 mg g−1 respectively. Weights of surviving larvae after 1 month on diets containing low concentrations of K-7 (0.1–1.0 mg g−1) were lower than on Btt or untreated larvae. K-7 was also toxic against larvae of C. cariosa and C. miliaris and found to harbour genes encoding Cry9Ea-like and Cry23Aa/Cry37Aa binary toxins. CONCLUSION Larvae of Capnodis spp. are susceptible to Bt Cry toxins. Expressing cry genes active against these pests thus seems a feasible solution towards production of transgenic rootstock trees resilient to the pest. 2014-06-13T02:08:21Z 2019-12-06T20:40:42Z 2014-06-13T02:08:21Z 2019-12-06T20:40:42Z 2013 2013 Journal Article Gindin, G., Mendel, Z., Levitin, B., Kumar, P., Levi, T., Shahi, P., et al. (2013). The basis for rootstock resilient to Capnodis species: screening for genes encoding δ -endotoxins from Bacillus thuringiensis . Pest Management Science, 70(8), 1283–1290. 1526-498X https://hdl.handle.net/10356/101569 http://hdl.handle.net/10220/19728 10.1002/ps.3694 en Pest management science © 2013 Society of Chemical Industry. |
| spellingShingle | DRNTU::Science::Biological sciences::Botany DRNTU::Engineering::Materials Gindin, Galina Mendel, Zvi Levitin, Bella Kumar, Pradeep Kuznetsova, Tatiana Levi, Tal Shahi, Preeti Khasdan, Vadim Weinthal, Dan Einav, Monica Kushmaro, Ariel Protasov, Alex Zaritsky, Arieh Ben-Dov, Eitan The basis for rootstock resilient to Capnodis species : screening for genes encoding δ-endotoxins from Bacillus thuringiensis |
| title | The basis for rootstock resilient to Capnodis species : screening for genes encoding δ-endotoxins from Bacillus thuringiensis |
| title_full | The basis for rootstock resilient to Capnodis species : screening for genes encoding δ-endotoxins from Bacillus thuringiensis |
| title_fullStr | The basis for rootstock resilient to Capnodis species : screening for genes encoding δ-endotoxins from Bacillus thuringiensis |
| title_full_unstemmed | The basis for rootstock resilient to Capnodis species : screening for genes encoding δ-endotoxins from Bacillus thuringiensis |
| title_short | The basis for rootstock resilient to Capnodis species : screening for genes encoding δ-endotoxins from Bacillus thuringiensis |
| title_sort | basis for rootstock resilient to capnodis species screening for genes encoding δ endotoxins from bacillus thuringiensis |
| topic | DRNTU::Science::Biological sciences::Botany DRNTU::Engineering::Materials |
| url | https://hdl.handle.net/10356/101569 http://hdl.handle.net/10220/19728 |
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