Human mesenchymal stem-cell behaviour on direct laser micropatterned electrospun scaffolds with hierarchical structures
Direct laser machining and electrospinning are utilized to obtain a bi-layered hybrid scaffold with hierarchical topographical features to mimic extracellular matrix-like microenvironment of cells. Adult bone marrow derived human mesenchymal stem cells (hMSCs) are cultured in vitro in these hybrid s...
| Main Authors: | , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2014
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/101674 http://hdl.handle.net/10220/19695 |
| _version_ | 1811691858297880576 |
|---|---|
| author | Li, Huaqiong Wong, Yee Shan Wen, Feng Ng, Kee Woei Ng, Gary Ka Lai Venkatraman, Subbu S. Boey, Freddy Yin Chiang Tan, Lay Poh |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Li, Huaqiong Wong, Yee Shan Wen, Feng Ng, Kee Woei Ng, Gary Ka Lai Venkatraman, Subbu S. Boey, Freddy Yin Chiang Tan, Lay Poh |
| author_sort | Li, Huaqiong |
| collection | NTU |
| description | Direct laser machining and electrospinning are utilized to obtain a bi-layered hybrid scaffold with hierarchical topographical features to mimic extracellular matrix-like microenvironment of cells. Adult bone marrow derived human mesenchymal stem cells (hMSCs) are cultured in vitro in these hybrid scaffolds, and cell orientation, proliferation, viability, and differentiation are evaluated. The results show that this novel hybrid scaffold not only supports cell growth like traditional scaffolds, but also elicits positive responses from the cells, like lineage commitment and alignment, which are essential features of future scaffolds. |
| first_indexed | 2024-10-01T06:26:34Z |
| format | Journal Article |
| id | ntu-10356/101674 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:26:34Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/1016742020-06-01T10:13:43Z Human mesenchymal stem-cell behaviour on direct laser micropatterned electrospun scaffolds with hierarchical structures Li, Huaqiong Wong, Yee Shan Wen, Feng Ng, Kee Woei Ng, Gary Ka Lai Venkatraman, Subbu S. Boey, Freddy Yin Chiang Tan, Lay Poh School of Materials Science & Engineering A*STAR SIMTech DRNTU::Science::Biological sciences::Molecular biology Direct laser machining and electrospinning are utilized to obtain a bi-layered hybrid scaffold with hierarchical topographical features to mimic extracellular matrix-like microenvironment of cells. Adult bone marrow derived human mesenchymal stem cells (hMSCs) are cultured in vitro in these hybrid scaffolds, and cell orientation, proliferation, viability, and differentiation are evaluated. The results show that this novel hybrid scaffold not only supports cell growth like traditional scaffolds, but also elicits positive responses from the cells, like lineage commitment and alignment, which are essential features of future scaffolds. 2014-06-12T06:07:41Z 2019-12-06T20:42:36Z 2014-06-12T06:07:41Z 2019-12-06T20:42:36Z 2013 2013 Journal Article Li, H., Wong, Y. S., Wen, F., Ng, K. W., Ng, G. K. L., Venkatraman, S. S., et al. (2013). Human Mesenchymal Stem-Cell Behaviour On Direct Laser Micropatterned Electrospun Scaffolds with Hierarchical Structures. Macromolecular Bioscience, 13(3), 299-310. 1616-5187 https://hdl.handle.net/10356/101674 http://hdl.handle.net/10220/19695 10.1002/mabi.201200318 en Macromolecular bioscience © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| spellingShingle | DRNTU::Science::Biological sciences::Molecular biology Li, Huaqiong Wong, Yee Shan Wen, Feng Ng, Kee Woei Ng, Gary Ka Lai Venkatraman, Subbu S. Boey, Freddy Yin Chiang Tan, Lay Poh Human mesenchymal stem-cell behaviour on direct laser micropatterned electrospun scaffolds with hierarchical structures |
| title | Human mesenchymal stem-cell behaviour on direct laser micropatterned electrospun scaffolds with hierarchical structures |
| title_full | Human mesenchymal stem-cell behaviour on direct laser micropatterned electrospun scaffolds with hierarchical structures |
| title_fullStr | Human mesenchymal stem-cell behaviour on direct laser micropatterned electrospun scaffolds with hierarchical structures |
| title_full_unstemmed | Human mesenchymal stem-cell behaviour on direct laser micropatterned electrospun scaffolds with hierarchical structures |
| title_short | Human mesenchymal stem-cell behaviour on direct laser micropatterned electrospun scaffolds with hierarchical structures |
| title_sort | human mesenchymal stem cell behaviour on direct laser micropatterned electrospun scaffolds with hierarchical structures |
| topic | DRNTU::Science::Biological sciences::Molecular biology |
| url | https://hdl.handle.net/10356/101674 http://hdl.handle.net/10220/19695 |
| work_keys_str_mv | AT lihuaqiong humanmesenchymalstemcellbehaviourondirectlasermicropatternedelectrospunscaffoldswithhierarchicalstructures AT wongyeeshan humanmesenchymalstemcellbehaviourondirectlasermicropatternedelectrospunscaffoldswithhierarchicalstructures AT wenfeng humanmesenchymalstemcellbehaviourondirectlasermicropatternedelectrospunscaffoldswithhierarchicalstructures AT ngkeewoei humanmesenchymalstemcellbehaviourondirectlasermicropatternedelectrospunscaffoldswithhierarchicalstructures AT nggarykalai humanmesenchymalstemcellbehaviourondirectlasermicropatternedelectrospunscaffoldswithhierarchicalstructures AT venkatramansubbus humanmesenchymalstemcellbehaviourondirectlasermicropatternedelectrospunscaffoldswithhierarchicalstructures AT boeyfreddyyinchiang humanmesenchymalstemcellbehaviourondirectlasermicropatternedelectrospunscaffoldswithhierarchicalstructures AT tanlaypoh humanmesenchymalstemcellbehaviourondirectlasermicropatternedelectrospunscaffoldswithhierarchicalstructures |