Optogenetics reveals a role for accumbal medium spiny neurons expressing dopamine D2 receptors in cocaine-induced behavioral sensitization
Long-lasting, drug-induced adaptations within the nucleus accumbens (NAc) have been proposed to contribute to drug-mediated addictive behaviors. Here we have used an optogenetic approach to examine the role of NAc medium spiny neurons (MSNs) expressing dopamine D2 receptors (D2Rs) in cocaine-induced...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2014
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| Online Access: | https://hdl.handle.net/10356/101853 http://hdl.handle.net/10220/24219 |
| _version_ | 1811686913021575168 |
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| author | Song, Shelly Sooyun Kang, Byeong Jun Wen, Lei Lee, Hyo Jin Sim, Hye-ri Kim, Tae Hyong Yoon, Sehyoun Yoon, Bong-June Augustine, George J. Baik, Ja-Hyun |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Song, Shelly Sooyun Kang, Byeong Jun Wen, Lei Lee, Hyo Jin Sim, Hye-ri Kim, Tae Hyong Yoon, Sehyoun Yoon, Bong-June Augustine, George J. Baik, Ja-Hyun |
| author_sort | Song, Shelly Sooyun |
| collection | NTU |
| description | Long-lasting, drug-induced adaptations within the nucleus accumbens (NAc) have been proposed to contribute to drug-mediated addictive behaviors. Here we have used an optogenetic approach to examine the role of NAc medium spiny neurons (MSNs) expressing dopamine D2 receptors (D2Rs) in cocaine-induced behavioral sensitization. Adeno-associated viral vectors encoding channelrhodopsin-2 (ChR2) were delivered into the NAc of D2R-Cre transgenic mice. This allowed us to selectively photostimulate D2R-MSNs in NAc. D2R-MSNs form local inhibitory circuits, because photostimulation of D2R-MSN evoked inhibitory postsynaptic currents (IPSCs) in neighboring MSNs. Photostimulation of NAc D2R-MSN in vivo affected neither the initiation nor the expression of cocaine-induced behavioral sensitization. However, photostimulation during the drug withdrawal period attenuated expression of cocaine-induced behavioral sensitization. These results show that D2R-MSNs of NAc play a key role in withdrawal-induced plasticity and may contribute to relapse after cessation of drug abuse. |
| first_indexed | 2024-10-01T05:07:58Z |
| format | Journal Article |
| id | ntu-10356/101853 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:07:58Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/1018532022-02-16T16:28:18Z Optogenetics reveals a role for accumbal medium spiny neurons expressing dopamine D2 receptors in cocaine-induced behavioral sensitization Song, Shelly Sooyun Kang, Byeong Jun Wen, Lei Lee, Hyo Jin Sim, Hye-ri Kim, Tae Hyong Yoon, Sehyoun Yoon, Bong-June Augustine, George J. Baik, Ja-Hyun Lee Kong Chian School of Medicine (LKCMedicine) DRNTU::Science::Biological sciences::Human anatomy and physiology::Neurobiology Long-lasting, drug-induced adaptations within the nucleus accumbens (NAc) have been proposed to contribute to drug-mediated addictive behaviors. Here we have used an optogenetic approach to examine the role of NAc medium spiny neurons (MSNs) expressing dopamine D2 receptors (D2Rs) in cocaine-induced behavioral sensitization. Adeno-associated viral vectors encoding channelrhodopsin-2 (ChR2) were delivered into the NAc of D2R-Cre transgenic mice. This allowed us to selectively photostimulate D2R-MSNs in NAc. D2R-MSNs form local inhibitory circuits, because photostimulation of D2R-MSN evoked inhibitory postsynaptic currents (IPSCs) in neighboring MSNs. Photostimulation of NAc D2R-MSN in vivo affected neither the initiation nor the expression of cocaine-induced behavioral sensitization. However, photostimulation during the drug withdrawal period attenuated expression of cocaine-induced behavioral sensitization. These results show that D2R-MSNs of NAc play a key role in withdrawal-induced plasticity and may contribute to relapse after cessation of drug abuse. Published version 2014-11-11T01:46:29Z 2019-12-06T20:45:43Z 2014-11-11T01:46:29Z 2019-12-06T20:45:43Z 2014 2014 Journal Article Song, S. S., Kang, B. J., Wen, L., Lee, H. J., Sim, H. r., Kim, T. H., et al. (2014). Optogenetics reveals a role for accumbal medium spiny neurons expressing dopamine D2 receptors in cocaine-induced behavioral sensitization. Frontiers in behavioral neuroscience, 8, 1-11. 1662-5153 https://hdl.handle.net/10356/101853 http://hdl.handle.net/10220/24219 10.3389/fnbeh.2014.00336 25352792 en Frontiers in behavioral neuroscience © 2014 Song, Kang, Wen, Lee, Sim, Kim, Yoon, Yoon, Augustine and Baik. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. 11 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Human anatomy and physiology::Neurobiology Song, Shelly Sooyun Kang, Byeong Jun Wen, Lei Lee, Hyo Jin Sim, Hye-ri Kim, Tae Hyong Yoon, Sehyoun Yoon, Bong-June Augustine, George J. Baik, Ja-Hyun Optogenetics reveals a role for accumbal medium spiny neurons expressing dopamine D2 receptors in cocaine-induced behavioral sensitization |
| title | Optogenetics reveals a role for accumbal medium spiny neurons expressing dopamine D2 receptors in cocaine-induced behavioral sensitization |
| title_full | Optogenetics reveals a role for accumbal medium spiny neurons expressing dopamine D2 receptors in cocaine-induced behavioral sensitization |
| title_fullStr | Optogenetics reveals a role for accumbal medium spiny neurons expressing dopamine D2 receptors in cocaine-induced behavioral sensitization |
| title_full_unstemmed | Optogenetics reveals a role for accumbal medium spiny neurons expressing dopamine D2 receptors in cocaine-induced behavioral sensitization |
| title_short | Optogenetics reveals a role for accumbal medium spiny neurons expressing dopamine D2 receptors in cocaine-induced behavioral sensitization |
| title_sort | optogenetics reveals a role for accumbal medium spiny neurons expressing dopamine d2 receptors in cocaine induced behavioral sensitization |
| topic | DRNTU::Science::Biological sciences::Human anatomy and physiology::Neurobiology |
| url | https://hdl.handle.net/10356/101853 http://hdl.handle.net/10220/24219 |
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