A defined co-culture of Geobacter sulfurreducens and Escherichia coli in a membrane-less microbial fuel cell
Wastewater-fed microbial fuel cells (MFCs) are a promising technology to treat low-organic carbon wastewater and recover part of the chemical energy in wastewater as electrical power. However, the interactions between electrochemically active and fermentative microorganisms cannot be easily studied...
| Main Authors: | , , |
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| Format: | Journal Article |
| Language: | English |
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2014
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| Online Access: | https://hdl.handle.net/10356/102030 http://hdl.handle.net/10220/18913 |
| _version_ | 1826124720906436608 |
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| author | Bourdakos, Nicholas Marsili, Enrico Mahadevan, Radhakrishnan |
| author2 | Singapore Centre for Environmental Life Sciences Engineering |
| author_facet | Singapore Centre for Environmental Life Sciences Engineering Bourdakos, Nicholas Marsili, Enrico Mahadevan, Radhakrishnan |
| author_sort | Bourdakos, Nicholas |
| collection | NTU |
| description | Wastewater-fed microbial fuel cells (MFCs) are a promising technology to treat low-organic carbon wastewater and recover part of the chemical energy in wastewater as electrical power. However, the interactions between electrochemically active and fermentative microorganisms cannot be easily studied in wastewater-fed MFCs because of their complex microbial communities. Defined co-culture MFCs provide a detailed understanding of such interactions. In this study, we characterize the extracellular metabolites in laboratory-scale membrane-less MFCs inoculated with Geobacter sulfurreducens and Escherichia coli co-culture and compare them with pure culture MFCs. G. sulfurreducens MFCs are sparged to maintain anaerobic conditions, while co-culture MFCs rely on E. coli for oxygen removal. G. sulfurreducens MFCs have a power output of 128 mW m−2, compared to 63 mW m−2 from the co-culture MFCs. Analysis of metabolites shows that succinate production in co-culture MFCs decreases current production by G. sulfurreducens and that the removal of succinate is responsible for the increased current density in the late co-culture MFCs. Interestingly, pH adjustment is not required for co-culture MFCs but a base addition is necessary for E. coli MFCs and cultures in vials. Our results show that defined co-culture MFCs provide clear insights into metabolic interactions among bacteria while maintaining a low operational complexity. |
| first_indexed | 2024-10-01T06:24:59Z |
| format | Journal Article |
| id | ntu-10356/102030 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:24:59Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/1020302020-03-07T12:47:11Z A defined co-culture of Geobacter sulfurreducens and Escherichia coli in a membrane-less microbial fuel cell Bourdakos, Nicholas Marsili, Enrico Mahadevan, Radhakrishnan Singapore Centre for Environmental Life Sciences Engineering DRNTU::Science::Biological sciences::Microbiology Wastewater-fed microbial fuel cells (MFCs) are a promising technology to treat low-organic carbon wastewater and recover part of the chemical energy in wastewater as electrical power. However, the interactions between electrochemically active and fermentative microorganisms cannot be easily studied in wastewater-fed MFCs because of their complex microbial communities. Defined co-culture MFCs provide a detailed understanding of such interactions. In this study, we characterize the extracellular metabolites in laboratory-scale membrane-less MFCs inoculated with Geobacter sulfurreducens and Escherichia coli co-culture and compare them with pure culture MFCs. G. sulfurreducens MFCs are sparged to maintain anaerobic conditions, while co-culture MFCs rely on E. coli for oxygen removal. G. sulfurreducens MFCs have a power output of 128 mW m−2, compared to 63 mW m−2 from the co-culture MFCs. Analysis of metabolites shows that succinate production in co-culture MFCs decreases current production by G. sulfurreducens and that the removal of succinate is responsible for the increased current density in the late co-culture MFCs. Interestingly, pH adjustment is not required for co-culture MFCs but a base addition is necessary for E. coli MFCs and cultures in vials. Our results show that defined co-culture MFCs provide clear insights into metabolic interactions among bacteria while maintaining a low operational complexity. 2014-03-17T06:14:56Z 2019-12-06T20:48:32Z 2014-03-17T06:14:56Z 2019-12-06T20:48:32Z 2013 2013 Journal Article Bourdakos, N., Marsili, E., & Mahadevan, R. (2014). A defined co-culture of Geobacter sulfurreducens and Escherichia coli in a membrane-less microbial fuel cell. Biotechnology and Bioengineering, 111(4), 709-718. 0006-3592 https://hdl.handle.net/10356/102030 http://hdl.handle.net/10220/18913 10.1002/bit.25137 en Biotechnology and bioengineering © 2013 Wiley Periodicals, Inc. |
| spellingShingle | DRNTU::Science::Biological sciences::Microbiology Bourdakos, Nicholas Marsili, Enrico Mahadevan, Radhakrishnan A defined co-culture of Geobacter sulfurreducens and Escherichia coli in a membrane-less microbial fuel cell |
| title | A defined co-culture of Geobacter sulfurreducens and Escherichia coli in a membrane-less microbial fuel cell |
| title_full | A defined co-culture of Geobacter sulfurreducens and Escherichia coli in a membrane-less microbial fuel cell |
| title_fullStr | A defined co-culture of Geobacter sulfurreducens and Escherichia coli in a membrane-less microbial fuel cell |
| title_full_unstemmed | A defined co-culture of Geobacter sulfurreducens and Escherichia coli in a membrane-less microbial fuel cell |
| title_short | A defined co-culture of Geobacter sulfurreducens and Escherichia coli in a membrane-less microbial fuel cell |
| title_sort | defined co culture of geobacter sulfurreducens and escherichia coli in a membrane less microbial fuel cell |
| topic | DRNTU::Science::Biological sciences::Microbiology |
| url | https://hdl.handle.net/10356/102030 http://hdl.handle.net/10220/18913 |
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