Distance-mediated plasmonic dimers for reusable colorimetric switches : a measurable peak shift of more than 60 nm
The first reconfigurable colorimetric DNA switches based on target DNA binding are reported. This DNA binding actuates a change in the interparticle distance between gold nanoparticle dimers. A significant spectral shift of 68 nm is achievable from on-off switching. The reconfigurability is possible...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2014
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/102132 http://hdl.handle.net/10220/18943 |
| _version_ | 1826122839282941952 |
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| author | Li, Chang Ming Guo, Longhua Ferhan, Abdul Rahim Chen, Hailan Chen, Guonan Hong, Seungpyo Kim, Dong-Hwan |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Li, Chang Ming Guo, Longhua Ferhan, Abdul Rahim Chen, Hailan Chen, Guonan Hong, Seungpyo Kim, Dong-Hwan |
| author_sort | Li, Chang Ming |
| collection | NTU |
| description | The first reconfigurable colorimetric DNA switches based on target DNA binding are reported. This DNA binding actuates a change in the interparticle distance between gold nanoparticle dimers. A significant spectral shift of 68 nm is achievable from on-off switching. The reconfigurability is possible owing to thiol and EDC-imidazole coupling which anchors the DNA linkers to the nanoparticles. The huge spectral shift allows the unaided eye to observe single target biomolecular binding event in real time under a darkfield microscope. The limit-of-detection for target molecules in PBS and human serum are 10−13 M and 10−11 M respectively. An improved fabrication strategy via asymmetric functionalization is also described, assisted by solid phase synthesis which minimizes the formation of trimers and multimers. |
| first_indexed | 2024-10-01T05:54:44Z |
| format | Journal Article |
| id | ntu-10356/102132 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:54:44Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/1021322020-03-07T11:35:25Z Distance-mediated plasmonic dimers for reusable colorimetric switches : a measurable peak shift of more than 60 nm Li, Chang Ming Guo, Longhua Ferhan, Abdul Rahim Chen, Hailan Chen, Guonan Hong, Seungpyo Kim, Dong-Hwan School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering The first reconfigurable colorimetric DNA switches based on target DNA binding are reported. This DNA binding actuates a change in the interparticle distance between gold nanoparticle dimers. A significant spectral shift of 68 nm is achievable from on-off switching. The reconfigurability is possible owing to thiol and EDC-imidazole coupling which anchors the DNA linkers to the nanoparticles. The huge spectral shift allows the unaided eye to observe single target biomolecular binding event in real time under a darkfield microscope. The limit-of-detection for target molecules in PBS and human serum are 10−13 M and 10−11 M respectively. An improved fabrication strategy via asymmetric functionalization is also described, assisted by solid phase synthesis which minimizes the formation of trimers and multimers. 2014-03-21T07:16:45Z 2019-12-06T20:50:08Z 2014-03-21T07:16:45Z 2019-12-06T20:50:08Z 2013 2013 Journal Article Guo, L., Ferhan, A. R., Chen, H., Li, C. M., Chen, G., Hong, S., et al. (2013). Distance-mediated plasmonic dimers for reusable colorimetric switches : a measurable peak shift of more than 60 nm. Small, 9(2), 234-240. 1613-6810 https://hdl.handle.net/10356/102132 http://hdl.handle.net/10220/18943 10.1002/smll.201201061 en Small © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| spellingShingle | DRNTU::Engineering::Chemical engineering Li, Chang Ming Guo, Longhua Ferhan, Abdul Rahim Chen, Hailan Chen, Guonan Hong, Seungpyo Kim, Dong-Hwan Distance-mediated plasmonic dimers for reusable colorimetric switches : a measurable peak shift of more than 60 nm |
| title | Distance-mediated plasmonic dimers for reusable colorimetric switches : a measurable peak shift of more than 60 nm |
| title_full | Distance-mediated plasmonic dimers for reusable colorimetric switches : a measurable peak shift of more than 60 nm |
| title_fullStr | Distance-mediated plasmonic dimers for reusable colorimetric switches : a measurable peak shift of more than 60 nm |
| title_full_unstemmed | Distance-mediated plasmonic dimers for reusable colorimetric switches : a measurable peak shift of more than 60 nm |
| title_short | Distance-mediated plasmonic dimers for reusable colorimetric switches : a measurable peak shift of more than 60 nm |
| title_sort | distance mediated plasmonic dimers for reusable colorimetric switches a measurable peak shift of more than 60 nm |
| topic | DRNTU::Engineering::Chemical engineering |
| url | https://hdl.handle.net/10356/102132 http://hdl.handle.net/10220/18943 |
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