Use of UTF1 genetic control elements as iPSC reporter
The reprogramming of adult somatic cells into an embryonic stem cell (ESC) state by various means has opened a new chapter in basic and applied life science. While this technology will create great opportunities for regenerative medicine, the more immediate impact is likely to be found in human dise...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Online Access: | https://hdl.handle.net/10356/102135 http://hdl.handle.net/10220/16803 |
| _version_ | 1811684081480499200 |
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| author | Morshedi, Amir Soroush Noghabi, Monireh Dröge, Peter |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Morshedi, Amir Soroush Noghabi, Monireh Dröge, Peter |
| author_sort | Morshedi, Amir |
| collection | NTU |
| description | The reprogramming of adult somatic cells into an embryonic stem cell (ESC) state by various means has opened a new chapter in basic and applied life science. While this technology will create great opportunities for regenerative medicine, the more immediate impact is likely to be found in human disease modeling and drug testing/development. An important aspect in the latter contexts is the ability to reliably monitor the pluripotent stem cell state, in particular with respect to human cell reprogramming using patient-specific somatic cells and high-throughput screens. Undifferentiated transcription factor 1 (UTF1) belongs to the core transcriptional network characterizing pluripotency. UTF1 is involved in ESC-specific chromatin organization, and its expression pattern during cell reprogramming and subsequent differentiation appears to be tightly connected with the pluripotent stem cell state. Here, we capitalized on these features and generated a reliable reporter system that was used to monitor induced pluripotent stem cell (iPSC) formation and subsequent differentiation. Our reporter cassette comprises less than 2.3 kb and remains functional during many cell passages after genomic integration. The fact that the human UTF1 genetic control elements work in a mouse background and the demonstrated functionality of the reporter in an epigenetic state further qualifies this system as a versatile new tool for iPSC research. |
| first_indexed | 2024-10-01T04:22:57Z |
| format | Journal Article |
| id | ntu-10356/102135 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:22:57Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/1021352020-03-07T12:18:09Z Use of UTF1 genetic control elements as iPSC reporter Morshedi, Amir Soroush Noghabi, Monireh Dröge, Peter School of Biological Sciences DRNTU::Science::Biological sciences The reprogramming of adult somatic cells into an embryonic stem cell (ESC) state by various means has opened a new chapter in basic and applied life science. While this technology will create great opportunities for regenerative medicine, the more immediate impact is likely to be found in human disease modeling and drug testing/development. An important aspect in the latter contexts is the ability to reliably monitor the pluripotent stem cell state, in particular with respect to human cell reprogramming using patient-specific somatic cells and high-throughput screens. Undifferentiated transcription factor 1 (UTF1) belongs to the core transcriptional network characterizing pluripotency. UTF1 is involved in ESC-specific chromatin organization, and its expression pattern during cell reprogramming and subsequent differentiation appears to be tightly connected with the pluripotent stem cell state. Here, we capitalized on these features and generated a reliable reporter system that was used to monitor induced pluripotent stem cell (iPSC) formation and subsequent differentiation. Our reporter cassette comprises less than 2.3 kb and remains functional during many cell passages after genomic integration. The fact that the human UTF1 genetic control elements work in a mouse background and the demonstrated functionality of the reporter in an epigenetic state further qualifies this system as a versatile new tool for iPSC research. 2013-10-24T07:45:27Z 2019-12-06T20:50:11Z 2013-10-24T07:45:27Z 2019-12-06T20:50:11Z 2011 2011 Journal Article Morshedi, A., Soroush Noghabi, M., & Dröge, P. (2011). Use of UTF1 genetic control elements as iPSC reporter. Stem cell reviews and reports, 9(4), 523-530. 1550-8943 https://hdl.handle.net/10356/102135 http://hdl.handle.net/10220/16803 10.1007/s12015-011-9342-7 en Stem cell reviews and reports |
| spellingShingle | DRNTU::Science::Biological sciences Morshedi, Amir Soroush Noghabi, Monireh Dröge, Peter Use of UTF1 genetic control elements as iPSC reporter |
| title | Use of UTF1 genetic control elements as iPSC reporter |
| title_full | Use of UTF1 genetic control elements as iPSC reporter |
| title_fullStr | Use of UTF1 genetic control elements as iPSC reporter |
| title_full_unstemmed | Use of UTF1 genetic control elements as iPSC reporter |
| title_short | Use of UTF1 genetic control elements as iPSC reporter |
| title_sort | use of utf1 genetic control elements as ipsc reporter |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/102135 http://hdl.handle.net/10220/16803 |
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