Dry powder inhaler formulation of lipid–polymer hybrid nanoparticles via electrostatically-driven nanoparticle assembly onto microscale carrier particles
Lipid–polymer hybrid nanoparticles have emerged as promising nanoscale carriers of therapeutics as they combine the attractive characteristics of liposomes and polymers. Herein we develop dry powder inhaler (DPI) formulation of hybrid nanoparticles composed of poly(lactic-co-glycolic acid) and soybe...
| Main Authors: | , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/102261 http://hdl.handle.net/10220/16847 |
| _version_ | 1811691938730999808 |
|---|---|
| author | Yang, Yue Cheow, Wean Sin Hadinoto, Kunn |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Yang, Yue Cheow, Wean Sin Hadinoto, Kunn |
| author_sort | Yang, Yue |
| collection | NTU |
| description | Lipid–polymer hybrid nanoparticles have emerged as promising nanoscale carriers of therapeutics as they combine the attractive characteristics of liposomes and polymers. Herein we develop dry powder inhaler (DPI) formulation of hybrid nanoparticles composed of poly(lactic-co-glycolic acid) and soybean lecithin as the polymer and lipid constituents, respectively. The hybrid nanoparticles are transformed into inhalable microscale nanocomposite structures by a novel technique based on electrostatically-driven adsorption of nanoparticles onto polysaccharide carrier particles, which eliminates the drawbacks of conventional techniques based on controlled drying (e.g. nanoparticle-specific formulation, low yield). First, we engineer polysaccharide carrier particles made up of chitosan cross-linked with tripolyphosphate and dextran sulphate to exhibit the desired aerosolization characteristics and physical robustness. Second, we investigate the effects of nanoparticle to carrier mass ratio and salt inclusion on the adsorption efficiency, in terms of the nanoparticle loading and yield, from which the optimal formulation is determined. Desorption of the nanoparticles from the carrier particles in phosphate buffer saline is also examined. Lastly, we characterize aerosolization efficiency of the nanocomposite product in vitro, where the emitted dose and respirable fraction are found to be comparable to the values of conventional DPI formulations. |
| first_indexed | 2024-10-01T06:27:51Z |
| format | Journal Article |
| id | ntu-10356/102261 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:27:51Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/1022612020-03-07T11:35:35Z Dry powder inhaler formulation of lipid–polymer hybrid nanoparticles via electrostatically-driven nanoparticle assembly onto microscale carrier particles Yang, Yue Cheow, Wean Sin Hadinoto, Kunn School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering Lipid–polymer hybrid nanoparticles have emerged as promising nanoscale carriers of therapeutics as they combine the attractive characteristics of liposomes and polymers. Herein we develop dry powder inhaler (DPI) formulation of hybrid nanoparticles composed of poly(lactic-co-glycolic acid) and soybean lecithin as the polymer and lipid constituents, respectively. The hybrid nanoparticles are transformed into inhalable microscale nanocomposite structures by a novel technique based on electrostatically-driven adsorption of nanoparticles onto polysaccharide carrier particles, which eliminates the drawbacks of conventional techniques based on controlled drying (e.g. nanoparticle-specific formulation, low yield). First, we engineer polysaccharide carrier particles made up of chitosan cross-linked with tripolyphosphate and dextran sulphate to exhibit the desired aerosolization characteristics and physical robustness. Second, we investigate the effects of nanoparticle to carrier mass ratio and salt inclusion on the adsorption efficiency, in terms of the nanoparticle loading and yield, from which the optimal formulation is determined. Desorption of the nanoparticles from the carrier particles in phosphate buffer saline is also examined. Lastly, we characterize aerosolization efficiency of the nanocomposite product in vitro, where the emitted dose and respirable fraction are found to be comparable to the values of conventional DPI formulations. 2013-10-24T09:01:46Z 2019-12-06T20:52:21Z 2013-10-24T09:01:46Z 2019-12-06T20:52:21Z 2012 2012 Journal Article Yang, Y., Cheow, W. S., & Hadinoto, K. (2012). Dry powder inhaler formulation of lipid–polymer hybrid nanoparticles via electrostatically-driven nanoparticle assembly onto microscale carrier particles. International journal of pharmaceutics, 434(1-2), 49-58. 0378-5173 https://hdl.handle.net/10356/102261 http://hdl.handle.net/10220/16847 10.1016/j.ijpharm.2012.05.036 en International journal of pharmaceutics |
| spellingShingle | DRNTU::Engineering::Chemical engineering Yang, Yue Cheow, Wean Sin Hadinoto, Kunn Dry powder inhaler formulation of lipid–polymer hybrid nanoparticles via electrostatically-driven nanoparticle assembly onto microscale carrier particles |
| title | Dry powder inhaler formulation of lipid–polymer hybrid nanoparticles via electrostatically-driven nanoparticle assembly onto microscale carrier particles |
| title_full | Dry powder inhaler formulation of lipid–polymer hybrid nanoparticles via electrostatically-driven nanoparticle assembly onto microscale carrier particles |
| title_fullStr | Dry powder inhaler formulation of lipid–polymer hybrid nanoparticles via electrostatically-driven nanoparticle assembly onto microscale carrier particles |
| title_full_unstemmed | Dry powder inhaler formulation of lipid–polymer hybrid nanoparticles via electrostatically-driven nanoparticle assembly onto microscale carrier particles |
| title_short | Dry powder inhaler formulation of lipid–polymer hybrid nanoparticles via electrostatically-driven nanoparticle assembly onto microscale carrier particles |
| title_sort | dry powder inhaler formulation of lipid polymer hybrid nanoparticles via electrostatically driven nanoparticle assembly onto microscale carrier particles |
| topic | DRNTU::Engineering::Chemical engineering |
| url | https://hdl.handle.net/10356/102261 http://hdl.handle.net/10220/16847 |
| work_keys_str_mv | AT yangyue drypowderinhalerformulationoflipidpolymerhybridnanoparticlesviaelectrostaticallydrivennanoparticleassemblyontomicroscalecarrierparticles AT cheowweansin drypowderinhalerformulationoflipidpolymerhybridnanoparticlesviaelectrostaticallydrivennanoparticleassemblyontomicroscalecarrierparticles AT hadinotokunn drypowderinhalerformulationoflipidpolymerhybridnanoparticlesviaelectrostaticallydrivennanoparticleassemblyontomicroscalecarrierparticles |