In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies
The dopamine receptor D2 (DRD2), a G-protein coupled receptor is expressed into PBd22-PEO13 and PMOXA20-PDMS54-PMOXA20 block copolymer vesicles (see scheme). The conformational integrity of the receptor is confirmed by antibody- and ligand-binding assays. Replacement of bound dopamine is demonstrate...
| Main Authors: | , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2014
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| Online Access: | https://hdl.handle.net/10356/102365 http://hdl.handle.net/10220/19016 |
| _version_ | 1826112639951962112 |
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| author | Low, Ying Xiu De Hoog, Hans-Peter M. May, Sylvia Andreasson-Ochsner, Mirjam Fu, Zhikang Tan, Darren Ritz, Sandra Nallani, Madhavan Sinner, Eva-Kathrin |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Low, Ying Xiu De Hoog, Hans-Peter M. May, Sylvia Andreasson-Ochsner, Mirjam Fu, Zhikang Tan, Darren Ritz, Sandra Nallani, Madhavan Sinner, Eva-Kathrin |
| author_sort | Low, Ying Xiu |
| collection | NTU |
| description | The dopamine receptor D2 (DRD2), a G-protein coupled receptor is expressed into PBd22-PEO13 and PMOXA20-PDMS54-PMOXA20 block copolymer vesicles (see scheme). The conformational integrity of the receptor is confirmed by antibody- and ligand-binding assays. Replacement of bound dopamine is demonstrated on surface-immobilized polymersomes, thus making this a promising platform for drug screening. |
| first_indexed | 2024-10-01T03:10:23Z |
| format | Journal Article |
| id | ntu-10356/102365 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:10:23Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/1023652020-06-01T10:13:45Z In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies Low, Ying Xiu De Hoog, Hans-Peter M. May, Sylvia Andreasson-Ochsner, Mirjam Fu, Zhikang Tan, Darren Ritz, Sandra Nallani, Madhavan Sinner, Eva-Kathrin School of Materials Science & Engineering DRNTU::Engineering::Materials::Biomaterials The dopamine receptor D2 (DRD2), a G-protein coupled receptor is expressed into PBd22-PEO13 and PMOXA20-PDMS54-PMOXA20 block copolymer vesicles (see scheme). The conformational integrity of the receptor is confirmed by antibody- and ligand-binding assays. Replacement of bound dopamine is demonstrated on surface-immobilized polymersomes, thus making this a promising platform for drug screening. 2014-03-27T09:06:11Z 2019-12-06T20:53:58Z 2014-03-27T09:06:11Z 2019-12-06T20:53:58Z 2013 2013 Journal Article May, S., Andreasson-Ochsner, M., Fu, Z., Low , Y. X., Tan, D., de Hoog, H.-P. M., et al. (2013). In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies. Angewandte Chemie International Edition, 52(2), 749-753. 1433-7851 https://hdl.handle.net/10356/102365 http://hdl.handle.net/10220/19016 10.1002/anie.201204645 en Angewandte chemie international edition © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| spellingShingle | DRNTU::Engineering::Materials::Biomaterials Low, Ying Xiu De Hoog, Hans-Peter M. May, Sylvia Andreasson-Ochsner, Mirjam Fu, Zhikang Tan, Darren Ritz, Sandra Nallani, Madhavan Sinner, Eva-Kathrin In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies |
| title | In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies |
| title_full | In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies |
| title_fullStr | In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies |
| title_full_unstemmed | In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies |
| title_short | In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies |
| title_sort | in vitro expressed gpcr inserted in polymersome membranes for ligand binding studies |
| topic | DRNTU::Engineering::Materials::Biomaterials |
| url | https://hdl.handle.net/10356/102365 http://hdl.handle.net/10220/19016 |
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