Electrochemical amplification strategies in DNA nanosensors
Electrochemical DNA nanosensors exploit the intrinsic property of ssDNA to hybridize to its complementary sequence and this selective hybridization event can be translated into detectable electrochemical signals following electrochemical amplification. This review describes some of the advanced elec...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2014
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| Online Access: | https://hdl.handle.net/10356/102449 http://hdl.handle.net/10220/18999 |
| _version_ | 1811688504871092224 |
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| author | Rai, Varun Toh, Chee-Seng |
| author2 | School of Physical and Mathematical Sciences |
| author_facet | School of Physical and Mathematical Sciences Rai, Varun Toh, Chee-Seng |
| author_sort | Rai, Varun |
| collection | NTU |
| description | Electrochemical DNA nanosensors exploit the intrinsic property of ssDNA to hybridize to its complementary sequence and this selective hybridization event can be translated into detectable electrochemical signals following electrochemical amplification. This review describes some of the advanced electrochemical signal amplification strategies in DNA detection relying on specific sequence hybridization with focus on electro-active intercalating reagents, primary and secondary reporter DNA probes and 'de-amplification' of signals derived from nanopores and nanochannels. These amplification strategies elegantly achieve extremely low detection limits of pico- to femtomolarity concentration and zeptomolar amount of DNAs and high specificity responses without the use of multi-step polymerase chain reaction (PCR) procedures or high sensitivity instrumentation. |
| first_indexed | 2024-10-01T05:33:16Z |
| format | Journal Article |
| id | ntu-10356/102449 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:33:16Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/1024492020-03-07T12:34:51Z Electrochemical amplification strategies in DNA nanosensors Rai, Varun Toh, Chee-Seng School of Physical and Mathematical Sciences DRNTU::Science::Chemistry::Analytical chemistry::Nucleic acids Electrochemical DNA nanosensors exploit the intrinsic property of ssDNA to hybridize to its complementary sequence and this selective hybridization event can be translated into detectable electrochemical signals following electrochemical amplification. This review describes some of the advanced electrochemical signal amplification strategies in DNA detection relying on specific sequence hybridization with focus on electro-active intercalating reagents, primary and secondary reporter DNA probes and 'de-amplification' of signals derived from nanopores and nanochannels. These amplification strategies elegantly achieve extremely low detection limits of pico- to femtomolarity concentration and zeptomolar amount of DNAs and high specificity responses without the use of multi-step polymerase chain reaction (PCR) procedures or high sensitivity instrumentation. 2014-03-27T08:00:54Z 2019-12-06T20:55:14Z 2014-03-27T08:00:54Z 2019-12-06T20:55:14Z 2013 2013 Journal Article Rai, V., & Toh, C.-S. (2013). Electrochemical amplification strategies in DNA nanosensors. Nanoscience and Nanotechnology Letters, 5(6), 613-623. https://hdl.handle.net/10356/102449 http://hdl.handle.net/10220/18999 10.1166/nnl.2013.1633 en Nanoscience and nanotechnology letters © 2013 American Scientific Publishers. |
| spellingShingle | DRNTU::Science::Chemistry::Analytical chemistry::Nucleic acids Rai, Varun Toh, Chee-Seng Electrochemical amplification strategies in DNA nanosensors |
| title | Electrochemical amplification strategies in DNA nanosensors |
| title_full | Electrochemical amplification strategies in DNA nanosensors |
| title_fullStr | Electrochemical amplification strategies in DNA nanosensors |
| title_full_unstemmed | Electrochemical amplification strategies in DNA nanosensors |
| title_short | Electrochemical amplification strategies in DNA nanosensors |
| title_sort | electrochemical amplification strategies in dna nanosensors |
| topic | DRNTU::Science::Chemistry::Analytical chemistry::Nucleic acids |
| url | https://hdl.handle.net/10356/102449 http://hdl.handle.net/10220/18999 |
| work_keys_str_mv | AT raivarun electrochemicalamplificationstrategiesindnananosensors AT tohcheeseng electrochemicalamplificationstrategiesindnananosensors |