Impact of short-term perfusion on cell retention for 3D bioconstruct development
Perfusion culture is a commonly used dynamic culture technique in tissue engineering for promoting higher cell number growth. Under perfusion culture, the cell number could be co-influenced by cell detachment and cell proliferation. However, previous studies have mainly focused on the perfusion effe...
| Main Authors: | , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2014
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/102721 http://hdl.handle.net/10220/19067 |
| _version_ | 1826130195969474560 |
|---|---|
| author | Liu, Dan Chua, Chee Kai Leong, Kah Fai |
| author2 | School of Mechanical and Aerospace Engineering |
| author_facet | School of Mechanical and Aerospace Engineering Liu, Dan Chua, Chee Kai Leong, Kah Fai |
| author_sort | Liu, Dan |
| collection | NTU |
| description | Perfusion culture is a commonly used dynamic culture technique in tissue engineering for promoting higher cell number growth. Under perfusion culture, the cell number could be co-influenced by cell detachment and cell proliferation. However, previous studies have mainly focused on the perfusion effects on cell proliferation but largely ignored the aspect of cell detachment. This work demonstrates that perfusion as small as 2.3 mL/min have induced cell loss compared with static culture even for a culture period of 12 minutes Both perfusion rate and direction have influenced the cell retention in the construct. In general, higher perfusion rates have caused more cell loss, largely due to the induced higher flow shear. The influence of perfusion directions on cell retention has been closely related to cell distribution in the construct. The study shows that cell retention is an important aspect that is worth more research attention and should be considered for cell number analysis in 3D construct development. |
| first_indexed | 2024-10-01T07:52:50Z |
| format | Journal Article |
| id | ntu-10356/102721 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:52:50Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/1027212020-03-07T13:22:21Z Impact of short-term perfusion on cell retention for 3D bioconstruct development Liu, Dan Chua, Chee Kai Leong, Kah Fai School of Mechanical and Aerospace Engineering DRNTU::Engineering::Materials Perfusion culture is a commonly used dynamic culture technique in tissue engineering for promoting higher cell number growth. Under perfusion culture, the cell number could be co-influenced by cell detachment and cell proliferation. However, previous studies have mainly focused on the perfusion effects on cell proliferation but largely ignored the aspect of cell detachment. This work demonstrates that perfusion as small as 2.3 mL/min have induced cell loss compared with static culture even for a culture period of 12 minutes Both perfusion rate and direction have influenced the cell retention in the construct. In general, higher perfusion rates have caused more cell loss, largely due to the induced higher flow shear. The influence of perfusion directions on cell retention has been closely related to cell distribution in the construct. The study shows that cell retention is an important aspect that is worth more research attention and should be considered for cell number analysis in 3D construct development. 2014-04-01T07:56:55Z 2019-12-06T20:59:35Z 2014-04-01T07:56:55Z 2019-12-06T20:59:35Z 2012 2012 Journal Article Liu, D., Chua, C. K., & Leong, K. F. (2013). Impact of short-term perfusion on cell retention for 3D bioconstruct development. Journal of Biomedical Materials Research Part A, 101A(3), 647-652. 1549-3296 https://hdl.handle.net/10356/102721 http://hdl.handle.net/10220/19067 10.1002/jbm.a.34366 en Journal of biomedical materials research part A © 2012 Wiley Periodicals, Inc. |
| spellingShingle | DRNTU::Engineering::Materials Liu, Dan Chua, Chee Kai Leong, Kah Fai Impact of short-term perfusion on cell retention for 3D bioconstruct development |
| title | Impact of short-term perfusion on cell retention for 3D bioconstruct development |
| title_full | Impact of short-term perfusion on cell retention for 3D bioconstruct development |
| title_fullStr | Impact of short-term perfusion on cell retention for 3D bioconstruct development |
| title_full_unstemmed | Impact of short-term perfusion on cell retention for 3D bioconstruct development |
| title_short | Impact of short-term perfusion on cell retention for 3D bioconstruct development |
| title_sort | impact of short term perfusion on cell retention for 3d bioconstruct development |
| topic | DRNTU::Engineering::Materials |
| url | https://hdl.handle.net/10356/102721 http://hdl.handle.net/10220/19067 |
| work_keys_str_mv | AT liudan impactofshorttermperfusiononcellretentionfor3dbioconstructdevelopment AT chuacheekai impactofshorttermperfusiononcellretentionfor3dbioconstructdevelopment AT leongkahfai impactofshorttermperfusiononcellretentionfor3dbioconstructdevelopment |