Third-party ATP sensing in polymersomes : a label-free assay of enzyme reactions in vesicular compartments
Submicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclid...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2014
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/102791 http://hdl.handle.net/10220/19139 |
| _version_ | 1826114084981964800 |
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| author | Yildiz, Umit Hakan De Hoog, Hans-Peter M. Fu, Zhikang Tomczak, Nikodem Parikh, Atul N. Nallani, Madhavan Liedberg, Bo |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Yildiz, Umit Hakan De Hoog, Hans-Peter M. Fu, Zhikang Tomczak, Nikodem Parikh, Atul N. Nallani, Madhavan Liedberg, Bo |
| author_sort | Yildiz, Umit Hakan |
| collection | NTU |
| description | Submicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclidin-1-ium. Passive diffusion of exogenously added adenosine triphosphate (ATP) through the membrane was sensed by monitoring the ATP-induced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase. |
| first_indexed | 2024-10-01T03:33:44Z |
| format | Journal Article |
| id | ntu-10356/102791 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:33:44Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/1027912020-06-01T10:26:41Z Third-party ATP sensing in polymersomes : a label-free assay of enzyme reactions in vesicular compartments Yildiz, Umit Hakan De Hoog, Hans-Peter M. Fu, Zhikang Tomczak, Nikodem Parikh, Atul N. Nallani, Madhavan Liedberg, Bo School of Materials Science & Engineering DRNTU::Engineering::Materials Submicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclidin-1-ium. Passive diffusion of exogenously added adenosine triphosphate (ATP) through the membrane was sensed by monitoring the ATP-induced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase. 2014-04-07T02:41:02Z 2019-12-06T21:00:14Z 2014-04-07T02:41:02Z 2019-12-06T21:00:14Z 2013 2013 Journal Article Yildiz, U. H., De Hoog, H.-P. M., Fu, Z., Tomczak, N., Parikh, A. N., Nallani, M., et al. (2014). Third-Party ATP Sensing in Polymersomes: A Label-Free Assay of Enzyme Reactions in Vesicular Compartments. Small, 10(3), 442-447. 1613-6810 https://hdl.handle.net/10356/102791 http://hdl.handle.net/10220/19139 10.1002/smll.201300060 en Small © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| spellingShingle | DRNTU::Engineering::Materials Yildiz, Umit Hakan De Hoog, Hans-Peter M. Fu, Zhikang Tomczak, Nikodem Parikh, Atul N. Nallani, Madhavan Liedberg, Bo Third-party ATP sensing in polymersomes : a label-free assay of enzyme reactions in vesicular compartments |
| title | Third-party ATP sensing in polymersomes : a label-free assay of enzyme reactions in vesicular compartments |
| title_full | Third-party ATP sensing in polymersomes : a label-free assay of enzyme reactions in vesicular compartments |
| title_fullStr | Third-party ATP sensing in polymersomes : a label-free assay of enzyme reactions in vesicular compartments |
| title_full_unstemmed | Third-party ATP sensing in polymersomes : a label-free assay of enzyme reactions in vesicular compartments |
| title_short | Third-party ATP sensing in polymersomes : a label-free assay of enzyme reactions in vesicular compartments |
| title_sort | third party atp sensing in polymersomes a label free assay of enzyme reactions in vesicular compartments |
| topic | DRNTU::Engineering::Materials |
| url | https://hdl.handle.net/10356/102791 http://hdl.handle.net/10220/19139 |
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