Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells
This study investigated the adhesive behaviors of normal and abnormal hematopoietic cells on nanotopographical materials. Previously, electrospun nanofiber scaffolds (NFSs) were used to capture and expand hematopoietic stem cells in vitro; here, we demonstrate that NFS could also serve as a useful b...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2014
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| Online Access: | https://hdl.handle.net/10356/103015 http://hdl.handle.net/10220/19151 |
| _version_ | 1811677029300436992 |
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| author | Chan, Jerry Kok Yen Cao, Xue Kwek, Kenneth Chan, Casey K. H. Lim, Mayasari |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Chan, Jerry Kok Yen Cao, Xue Kwek, Kenneth Chan, Casey K. H. Lim, Mayasari |
| author_sort | Chan, Jerry Kok Yen |
| collection | NTU |
| description | This study investigated the adhesive behaviors of normal and abnormal hematopoietic cells on nanotopographical materials. Previously, electrospun nanofiber scaffolds (NFSs) were used to capture and expand hematopoietic stem cells in vitro; here, we demonstrate that NFS could also serve as a useful bioadhesive platform for capturing functionally adherent leukemia cells. Collagen-blended poly(d,l-lactide-co-glycolide) NFS enabled more rapid and efficient capture of K562 leukemia cells than tissue culture polystyrene surfaces with up to 70% improved adhesion and shorter time. Cellular extensions, stronger adhesion, and enhanced cell–cell interactions were observed in K562 cells captured on NFS. While NFS promoted hematopoietic progenitor cell proliferation, it inhibited leukemia cell proliferation and affected cell cycle status by shifting more cells toward the G0/G1 phase. The expression of α-integrins was equally high in both captured and uncaptured leukemia cell populations demonstrating no relation to its adhesive nature. Hematopoietic morphological signatures of NFS captured cells presented no impact on cell differentiation. We conclude that electrospun NFS serves as an excellent platform not only for capturing functionally adherent leukemia cells but also for studying the impact of niche-like structure in the nanoscale. |
| first_indexed | 2024-10-01T02:30:52Z |
| format | Journal Article |
| id | ntu-10356/103015 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:30:52Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/1030152020-03-07T11:35:36Z Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells Chan, Jerry Kok Yen Cao, Xue Kwek, Kenneth Chan, Casey K. H. Lim, Mayasari School of Chemical and Biomedical Engineering DRNTU::Science::Chemistry This study investigated the adhesive behaviors of normal and abnormal hematopoietic cells on nanotopographical materials. Previously, electrospun nanofiber scaffolds (NFSs) were used to capture and expand hematopoietic stem cells in vitro; here, we demonstrate that NFS could also serve as a useful bioadhesive platform for capturing functionally adherent leukemia cells. Collagen-blended poly(d,l-lactide-co-glycolide) NFS enabled more rapid and efficient capture of K562 leukemia cells than tissue culture polystyrene surfaces with up to 70% improved adhesion and shorter time. Cellular extensions, stronger adhesion, and enhanced cell–cell interactions were observed in K562 cells captured on NFS. While NFS promoted hematopoietic progenitor cell proliferation, it inhibited leukemia cell proliferation and affected cell cycle status by shifting more cells toward the G0/G1 phase. The expression of α-integrins was equally high in both captured and uncaptured leukemia cell populations demonstrating no relation to its adhesive nature. Hematopoietic morphological signatures of NFS captured cells presented no impact on cell differentiation. We conclude that electrospun NFS serves as an excellent platform not only for capturing functionally adherent leukemia cells but also for studying the impact of niche-like structure in the nanoscale. 2014-04-07T05:12:25Z 2019-12-06T21:03:59Z 2014-04-07T05:12:25Z 2019-12-06T21:03:59Z 2013 2013 Journal Article Cao, X., Kwek, K., Chan, J. K. Y., Chan, C. K. H., & Lim, M. (2014). Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells. Journal of Biomedical Materials Research Part A, 102(2), 523-531. 1549-3296 https://hdl.handle.net/10356/103015 http://hdl.handle.net/10220/19151 10.1002/jbm.a.34716 en Journal of biomedical materials research part A © 2013 Wiley Periodicals, Inc. |
| spellingShingle | DRNTU::Science::Chemistry Chan, Jerry Kok Yen Cao, Xue Kwek, Kenneth Chan, Casey K. H. Lim, Mayasari Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells |
| title | Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells |
| title_full | Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells |
| title_fullStr | Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells |
| title_full_unstemmed | Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells |
| title_short | Electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells |
| title_sort | electrospun nanofibers as a bioadhesive platform for capturing adherent leukemia cells |
| topic | DRNTU::Science::Chemistry |
| url | https://hdl.handle.net/10356/103015 http://hdl.handle.net/10220/19151 |
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