Phaeobacter gallaeciensis genomes from globally opposite locations reveal high similarity of adaptation to surface life
Phaeobacter gallaeciensis, a member of the abundant marine Roseobacter clade, is known to be an effective colonizer of biotic and abiotic marine surfaces. Production of the antibiotic tropodithietic acid (TDA) makes P. gallaeciensis a strong antagonist of many bacteria, including fish and mollusc pa...
| Autors principals: | , , , , , , , , , , , |
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| Altres autors: | |
| Format: | Journal Article |
| Idioma: | English |
| Publicat: |
2013
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| Accés en línia: | https://hdl.handle.net/10356/103140 http://hdl.handle.net/10220/16900 |
| _version_ | 1826121261134118912 |
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| author | Thole, Sebastian Kalhoefer, Daniela Voget, Sonja Berger, Martine Engelhardt, Tim Liesegang, Heiko Wollherr, Antje Kjelleberg, Staffan Daniel, Rolf Simon, Meinhard Thomas, Torsten Brinkhoff, Thorsten |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Thole, Sebastian Kalhoefer, Daniela Voget, Sonja Berger, Martine Engelhardt, Tim Liesegang, Heiko Wollherr, Antje Kjelleberg, Staffan Daniel, Rolf Simon, Meinhard Thomas, Torsten Brinkhoff, Thorsten |
| author_sort | Thole, Sebastian |
| collection | NTU |
| description | Phaeobacter gallaeciensis, a member of the abundant marine Roseobacter clade, is known to be an effective colonizer of biotic and abiotic marine surfaces. Production of the antibiotic tropodithietic acid (TDA) makes P. gallaeciensis a strong antagonist of many bacteria, including fish and mollusc pathogens. In addition to TDA, several other secondary metabolites are produced, allowing the mutualistic bacterium to also act as an opportunistic pathogen. Here we provide the manually annotated genome sequences of the P. gallaeciensis strains DSM 17395 and 2.10, isolated at the Atlantic coast of north western Spain and near Sydney, Australia, respectively. Despite their isolation sites from the two different hemispheres, the genome comparison demonstrated a surprisingly high level of synteny (only 3% nucleotide dissimilarity and 88% and 93% shared genes). Minor differences in the genomes result from horizontal gene transfer and phage infection. Comparison of the P. gallaeciensis genomes with those of other roseobacters revealed unique genomic traits, including the production of iron-scavenging siderophores. Experiments supported the predicted capacity of both strains to grow on various algal osmolytes. Transposon mutagenesis was used to expand the current knowledge on the TDA biosynthesis pathway in strain DSM 17395. This first comparative genomic analysis of finished genomes of two closely related strains belonging to one species of the Roseobacter clade revealed features that provide competitive advantages and facilitate surface attachment and interaction with eukaryotic hosts. |
| first_indexed | 2024-10-01T05:29:48Z |
| format | Journal Article |
| id | ntu-10356/103140 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:29:48Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/1031402022-02-16T16:28:08Z Phaeobacter gallaeciensis genomes from globally opposite locations reveal high similarity of adaptation to surface life Thole, Sebastian Kalhoefer, Daniela Voget, Sonja Berger, Martine Engelhardt, Tim Liesegang, Heiko Wollherr, Antje Kjelleberg, Staffan Daniel, Rolf Simon, Meinhard Thomas, Torsten Brinkhoff, Thorsten School of Biological Sciences DRNTU::Science::Biological sciences Phaeobacter gallaeciensis, a member of the abundant marine Roseobacter clade, is known to be an effective colonizer of biotic and abiotic marine surfaces. Production of the antibiotic tropodithietic acid (TDA) makes P. gallaeciensis a strong antagonist of many bacteria, including fish and mollusc pathogens. In addition to TDA, several other secondary metabolites are produced, allowing the mutualistic bacterium to also act as an opportunistic pathogen. Here we provide the manually annotated genome sequences of the P. gallaeciensis strains DSM 17395 and 2.10, isolated at the Atlantic coast of north western Spain and near Sydney, Australia, respectively. Despite their isolation sites from the two different hemispheres, the genome comparison demonstrated a surprisingly high level of synteny (only 3% nucleotide dissimilarity and 88% and 93% shared genes). Minor differences in the genomes result from horizontal gene transfer and phage infection. Comparison of the P. gallaeciensis genomes with those of other roseobacters revealed unique genomic traits, including the production of iron-scavenging siderophores. Experiments supported the predicted capacity of both strains to grow on various algal osmolytes. Transposon mutagenesis was used to expand the current knowledge on the TDA biosynthesis pathway in strain DSM 17395. This first comparative genomic analysis of finished genomes of two closely related strains belonging to one species of the Roseobacter clade revealed features that provide competitive advantages and facilitate surface attachment and interaction with eukaryotic hosts. 2013-10-25T03:16:37Z 2019-12-06T21:06:25Z 2013-10-25T03:16:37Z 2019-12-06T21:06:25Z 2012 2012 Journal Article Thole, S., Kalhoefer, D., Voget, S., Berger, M., Engelhardt, T., Liesegang, H., et al. (2012). Phaeobacter gallaeciensis genomes from globally opposite locations reveal high similarity of adaptation to surface life. The ISME Journal, 6, 2229-2244. https://hdl.handle.net/10356/103140 http://hdl.handle.net/10220/16900 10.1038/ismej.2012.62 22717884 en The ISME journal © 2012 International Society for Microbial Ecology. |
| spellingShingle | DRNTU::Science::Biological sciences Thole, Sebastian Kalhoefer, Daniela Voget, Sonja Berger, Martine Engelhardt, Tim Liesegang, Heiko Wollherr, Antje Kjelleberg, Staffan Daniel, Rolf Simon, Meinhard Thomas, Torsten Brinkhoff, Thorsten Phaeobacter gallaeciensis genomes from globally opposite locations reveal high similarity of adaptation to surface life |
| title | Phaeobacter gallaeciensis genomes from globally opposite locations reveal high similarity of adaptation to surface life |
| title_full | Phaeobacter gallaeciensis genomes from globally opposite locations reveal high similarity of adaptation to surface life |
| title_fullStr | Phaeobacter gallaeciensis genomes from globally opposite locations reveal high similarity of adaptation to surface life |
| title_full_unstemmed | Phaeobacter gallaeciensis genomes from globally opposite locations reveal high similarity of adaptation to surface life |
| title_short | Phaeobacter gallaeciensis genomes from globally opposite locations reveal high similarity of adaptation to surface life |
| title_sort | phaeobacter gallaeciensis genomes from globally opposite locations reveal high similarity of adaptation to surface life |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/103140 http://hdl.handle.net/10220/16900 |
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