The Pseudomonas aeruginosa Type III translocon is required for biofilm formation at the epithelial barrier
Clinical infections by Pseudomonas aeruginosa, a deadly Gram-negative, opportunistic pathogen of immunocompromised hosts, often involve the formation of antibiotic-resistant biofilms. Although biofilm formation has been extensively studied in vitro on glass or plastic surfaces, much less is known ab...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2014
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| Online Access: | https://hdl.handle.net/10356/103191 http://hdl.handle.net/10220/24407 |
| _version_ | 1811678151322894336 |
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| author | Tran, Cindy S. Rangel, Stephanie M. Almblad, Henrik Kierbel, Arlinet Givskov, Michael Tolker-Nielsen, Tim Hauser, Alan R. Engel, Joanne N. |
| author2 | Yahr, Timothy L. |
| author_facet | Yahr, Timothy L. Tran, Cindy S. Rangel, Stephanie M. Almblad, Henrik Kierbel, Arlinet Givskov, Michael Tolker-Nielsen, Tim Hauser, Alan R. Engel, Joanne N. |
| author_sort | Tran, Cindy S. |
| collection | NTU |
| description | Clinical infections by Pseudomonas aeruginosa, a deadly Gram-negative, opportunistic pathogen of immunocompromised hosts, often involve the formation of antibiotic-resistant biofilms. Although biofilm formation has been extensively studied in vitro on glass or plastic surfaces, much less is known about biofilm formation at the epithelial barrier. We have previously shown that when added to the apical surface of polarized epithelial cells, P. aeruginosa rapidly forms cell-associated aggregates within 60 minutes of infection. By confocal microscopy we now show that cell-associated aggregates exhibit key characteristics of biofilms, including the presence of extracellular matrix and increased resistance to antibiotics compared to planktonic bacteria. Using isogenic mutants in the type III secretion system, we found that the translocon, but not the effectors themselves, were required for cell-associated aggregation on the surface of polarized epithelial cells and at early time points in a murine model of acute pneumonia. In contrast, the translocon was not required for aggregation on abiotic surfaces, suggesting a novel function for the type III secretion system during cell-associated aggregation. Supernatants from epithelial cells infected with wild-type bacteria or from cells treated with the pore-forming toxin streptolysin O could rescue aggregate formation in a type III secretion mutant, indicating that cell-associated aggregation requires one or more host cell factors. Our results suggest a previously unappreciated function for the type III translocon in the formation of P. aeruginosa biofilms at the epithelial barrier and demonstrate that biofilms may form at early time points of infection. |
| first_indexed | 2024-10-01T02:48:42Z |
| format | Journal Article |
| id | ntu-10356/103191 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:48:42Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/1031912022-02-16T16:29:35Z The Pseudomonas aeruginosa Type III translocon is required for biofilm formation at the epithelial barrier Tran, Cindy S. Rangel, Stephanie M. Almblad, Henrik Kierbel, Arlinet Givskov, Michael Tolker-Nielsen, Tim Hauser, Alan R. Engel, Joanne N. Yahr, Timothy L. Singapore Centre for Environmental Life Sciences Engineering DRNTU::Science::Biological sciences Clinical infections by Pseudomonas aeruginosa, a deadly Gram-negative, opportunistic pathogen of immunocompromised hosts, often involve the formation of antibiotic-resistant biofilms. Although biofilm formation has been extensively studied in vitro on glass or plastic surfaces, much less is known about biofilm formation at the epithelial barrier. We have previously shown that when added to the apical surface of polarized epithelial cells, P. aeruginosa rapidly forms cell-associated aggregates within 60 minutes of infection. By confocal microscopy we now show that cell-associated aggregates exhibit key characteristics of biofilms, including the presence of extracellular matrix and increased resistance to antibiotics compared to planktonic bacteria. Using isogenic mutants in the type III secretion system, we found that the translocon, but not the effectors themselves, were required for cell-associated aggregation on the surface of polarized epithelial cells and at early time points in a murine model of acute pneumonia. In contrast, the translocon was not required for aggregation on abiotic surfaces, suggesting a novel function for the type III secretion system during cell-associated aggregation. Supernatants from epithelial cells infected with wild-type bacteria or from cells treated with the pore-forming toxin streptolysin O could rescue aggregate formation in a type III secretion mutant, indicating that cell-associated aggregation requires one or more host cell factors. Our results suggest a previously unappreciated function for the type III translocon in the formation of P. aeruginosa biofilms at the epithelial barrier and demonstrate that biofilms may form at early time points of infection. Published version 2014-12-09T08:57:23Z 2019-12-06T21:07:07Z 2014-12-09T08:57:23Z 2019-12-06T21:07:07Z 2014 2014 Journal Article Tran, C. S., Rangel, S. M., Almblad, H., Kierbel, A., Givskov, M., Tolker-Nielsen, T., et al. (2014). The Pseudomonas aeruginosa Type III translocon is required for biofilm formation at the epithelial barrier. PLoS Pathogens, 10(11), e1004479-. 1553-7374 https://hdl.handle.net/10356/103191 http://hdl.handle.net/10220/24407 10.1371/journal.ppat.1004479 25375398 en PLoS Pathogens © 2014 Tran et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Tran, Cindy S. Rangel, Stephanie M. Almblad, Henrik Kierbel, Arlinet Givskov, Michael Tolker-Nielsen, Tim Hauser, Alan R. Engel, Joanne N. The Pseudomonas aeruginosa Type III translocon is required for biofilm formation at the epithelial barrier |
| title | The Pseudomonas aeruginosa Type III translocon is required for biofilm formation at the epithelial barrier |
| title_full | The Pseudomonas aeruginosa Type III translocon is required for biofilm formation at the epithelial barrier |
| title_fullStr | The Pseudomonas aeruginosa Type III translocon is required for biofilm formation at the epithelial barrier |
| title_full_unstemmed | The Pseudomonas aeruginosa Type III translocon is required for biofilm formation at the epithelial barrier |
| title_short | The Pseudomonas aeruginosa Type III translocon is required for biofilm formation at the epithelial barrier |
| title_sort | pseudomonas aeruginosa type iii translocon is required for biofilm formation at the epithelial barrier |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/103191 http://hdl.handle.net/10220/24407 |
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