Free-standing hydrogel-particle composite membrane with dynamically controlled permeability
The preparation and investigation of a free-standing membrane made from a composite of thermoresponsive poly(N-isopropylacrylamide) (pNIPAAm) and polystyrene nanoparticles (PS NP) with temperature-controlled permeability is reported. The method exploits the light-induced crosslinking of the photo-re...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2018
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| Online Access: | https://hdl.handle.net/10356/103317 http://hdl.handle.net/10220/47279 |
| _version_ | 1826123528289648640 |
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| author | Sergelen, Khulan Petri, Christian Jonas, Ulrich Dostalek, Jakub |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Sergelen, Khulan Petri, Christian Jonas, Ulrich Dostalek, Jakub |
| author_sort | Sergelen, Khulan |
| collection | NTU |
| description | The preparation and investigation of a free-standing membrane made from a composite of thermoresponsive poly(N-isopropylacrylamide) (pNIPAAm) and polystyrene nanoparticles (PS NP) with temperature-controlled permeability is reported. The method exploits the light-induced crosslinking of the photo-reactive pNIPAAm-based polymer and mechanical reinforcement of the membrane structure by the polystyrene nanoparticles. About micrometer thick layers were either directly attached to a gold surface or prepared as free-standing layers spanning over arrays of microfluidic channels with a width of about hundred microns by using template stripping. Diffusion of liquid medium, low molecular weight molecules, and large molecular weight proteins contained in blood through the composite membrane was observed with combined surface plasmon resonance (SPR) and optical waveguide spectroscopy (OWS). The swelling ratio, permeability, and nonspecific sorption to these composite membranes were investigated by SPR and OWS as a function of molecular weight of analyte, loading of PS NP in the composite film, and temperature. The authors show successful preparation of a defect-free membrane structure that acts as a thermoresponsive filter with nanoscale pores spanning over an area of several square millimeters. This membrane can be reversibly switched to block or allow the diffusion of low mass molecules to the sensor surface by temperature-triggered swelling and collapsing of the hydrogel component. Blocking of diffusion and low unspecific sorption of proteins contained in blood serum is observed. These features make this platform interesting for potential future applications in continuous monitoring biosensors for the analysis of low molecular weight drug analytes or for advanced cell-on-chip microfluidic studies. |
| first_indexed | 2024-10-01T06:06:09Z |
| format | Journal Article |
| id | ntu-10356/103317 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:06:09Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/1033172023-07-14T15:55:36Z Free-standing hydrogel-particle composite membrane with dynamically controlled permeability Sergelen, Khulan Petri, Christian Jonas, Ulrich Dostalek, Jakub School of Materials Science & Engineering Centre for Biomimetic Sensor Science Biosensors DRNTU::Engineering::Materials Biofluids The preparation and investigation of a free-standing membrane made from a composite of thermoresponsive poly(N-isopropylacrylamide) (pNIPAAm) and polystyrene nanoparticles (PS NP) with temperature-controlled permeability is reported. The method exploits the light-induced crosslinking of the photo-reactive pNIPAAm-based polymer and mechanical reinforcement of the membrane structure by the polystyrene nanoparticles. About micrometer thick layers were either directly attached to a gold surface or prepared as free-standing layers spanning over arrays of microfluidic channels with a width of about hundred microns by using template stripping. Diffusion of liquid medium, low molecular weight molecules, and large molecular weight proteins contained in blood through the composite membrane was observed with combined surface plasmon resonance (SPR) and optical waveguide spectroscopy (OWS). The swelling ratio, permeability, and nonspecific sorption to these composite membranes were investigated by SPR and OWS as a function of molecular weight of analyte, loading of PS NP in the composite film, and temperature. The authors show successful preparation of a defect-free membrane structure that acts as a thermoresponsive filter with nanoscale pores spanning over an area of several square millimeters. This membrane can be reversibly switched to block or allow the diffusion of low mass molecules to the sensor surface by temperature-triggered swelling and collapsing of the hydrogel component. Blocking of diffusion and low unspecific sorption of proteins contained in blood serum is observed. These features make this platform interesting for potential future applications in continuous monitoring biosensors for the analysis of low molecular weight drug analytes or for advanced cell-on-chip microfluidic studies. Published version 2018-12-28T07:33:24Z 2019-12-06T21:09:48Z 2018-12-28T07:33:24Z 2019-12-06T21:09:48Z 2017 Journal Article Sergelen, K., Petri, C., Jonas, U., & Dostalek, J. (2017). Free-standing hydrogel-particle composite membrane with dynamically controlled permeability. Biointerphases, 12(5), 051002-. doi:10.1116/1.4996952 1934-8630 https://hdl.handle.net/10356/103317 http://hdl.handle.net/10220/47279 10.1116/1.4996952 en Biointerphases © 2017 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). 9 p. application/pdf |
| spellingShingle | Biosensors DRNTU::Engineering::Materials Biofluids Sergelen, Khulan Petri, Christian Jonas, Ulrich Dostalek, Jakub Free-standing hydrogel-particle composite membrane with dynamically controlled permeability |
| title | Free-standing hydrogel-particle composite membrane with dynamically controlled permeability |
| title_full | Free-standing hydrogel-particle composite membrane with dynamically controlled permeability |
| title_fullStr | Free-standing hydrogel-particle composite membrane with dynamically controlled permeability |
| title_full_unstemmed | Free-standing hydrogel-particle composite membrane with dynamically controlled permeability |
| title_short | Free-standing hydrogel-particle composite membrane with dynamically controlled permeability |
| title_sort | free standing hydrogel particle composite membrane with dynamically controlled permeability |
| topic | Biosensors DRNTU::Engineering::Materials Biofluids |
| url | https://hdl.handle.net/10356/103317 http://hdl.handle.net/10220/47279 |
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