Global regulator morA affects virulence-associated protease secretion in pseudomonas aeruginosa PAO1
Bacterial invasion plays a critical role in the establishment of Pseudomonas aeruginosa infection and is aided by two major virulence factors – surface appendages and secreted proteases. The second messenger cyclic diguanylate (c-di-GMP) is known to affect bacterial attachment to surfaces, biofilm f...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2015
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| Online Access: | https://hdl.handle.net/10356/103501 http://hdl.handle.net/10220/25829 |
| _version_ | 1811677763079241728 |
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| author | Swarup, Sanjay Ravichandran, Ayshwarya Ramachandran, Malarmathy Suriyanarayanan, Tanujaa Wong, Chui Ching |
| author2 | Singapore Centre for Environmental Life Sciences Engineering |
| author_facet | Singapore Centre for Environmental Life Sciences Engineering Swarup, Sanjay Ravichandran, Ayshwarya Ramachandran, Malarmathy Suriyanarayanan, Tanujaa Wong, Chui Ching |
| author_sort | Swarup, Sanjay |
| collection | NTU |
| description | Bacterial invasion plays a critical role in the establishment of Pseudomonas aeruginosa infection and is aided by two major virulence factors – surface appendages and secreted proteases. The second messenger cyclic diguanylate (c-di-GMP) is known to affect bacterial attachment to surfaces, biofilm formation and related virulence phenomena. Here we report that MorA, a global regulator with GGDEF and EAL domains that was previously reported to affect virulence factors, negatively regulates protease secretion via the type II secretion system (T2SS) in P. aeruginosa PAO1. Infection assays with mutant strains carrying gene deletion and domain mutants show that host cell invasion is dependent on the active domain function of MorA. Further investigations suggest that the MorA-mediated c-di-GMP signaling affects protease secretion largely at a post-translational level. We thus report c-di-GMP second messenger system as a novel regulator of T2SS function in P. aeruginosa. Given that T2SS is a central and constitutive pump, and the secreted proteases are involved in interactions with the microbial surroundings, our data broadens the significance of c-di-GMP signaling in P. aeruginosa pathogenesis and ecological fitness. |
| first_indexed | 2024-10-01T02:42:32Z |
| format | Journal Article |
| id | ntu-10356/103501 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:42:32Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/1035012022-02-16T16:27:17Z Global regulator morA affects virulence-associated protease secretion in pseudomonas aeruginosa PAO1 Swarup, Sanjay Ravichandran, Ayshwarya Ramachandran, Malarmathy Suriyanarayanan, Tanujaa Wong, Chui Ching Singapore Centre for Environmental Life Sciences Engineering DRNTU::Science::Biological sciences::Microbiology::Bacteria Bacterial invasion plays a critical role in the establishment of Pseudomonas aeruginosa infection and is aided by two major virulence factors – surface appendages and secreted proteases. The second messenger cyclic diguanylate (c-di-GMP) is known to affect bacterial attachment to surfaces, biofilm formation and related virulence phenomena. Here we report that MorA, a global regulator with GGDEF and EAL domains that was previously reported to affect virulence factors, negatively regulates protease secretion via the type II secretion system (T2SS) in P. aeruginosa PAO1. Infection assays with mutant strains carrying gene deletion and domain mutants show that host cell invasion is dependent on the active domain function of MorA. Further investigations suggest that the MorA-mediated c-di-GMP signaling affects protease secretion largely at a post-translational level. We thus report c-di-GMP second messenger system as a novel regulator of T2SS function in P. aeruginosa. Given that T2SS is a central and constitutive pump, and the secreted proteases are involved in interactions with the microbial surroundings, our data broadens the significance of c-di-GMP signaling in P. aeruginosa pathogenesis and ecological fitness. Published version 2015-06-08T05:05:27Z 2019-12-06T21:14:04Z 2015-06-08T05:05:27Z 2019-12-06T21:14:04Z 2015 2015 Journal Article Ravichandran, A., Ramachandran, M., Suriyanarayanan, T., Wong, C. C., & Swarup, S. (2015). Global regulator morA affects virulence-associated protease secretion in pseudomonas aeruginosa PAO1. PLOS One, 10(4), e0123805-. 1932-6203 https://hdl.handle.net/10356/103501 http://hdl.handle.net/10220/25829 10.1371/journal.pone.0123805 25894344 en PLOS One © 2015 Ravichandran et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 17 p. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Microbiology::Bacteria Swarup, Sanjay Ravichandran, Ayshwarya Ramachandran, Malarmathy Suriyanarayanan, Tanujaa Wong, Chui Ching Global regulator morA affects virulence-associated protease secretion in pseudomonas aeruginosa PAO1 |
| title | Global regulator morA affects virulence-associated protease secretion in pseudomonas aeruginosa PAO1 |
| title_full | Global regulator morA affects virulence-associated protease secretion in pseudomonas aeruginosa PAO1 |
| title_fullStr | Global regulator morA affects virulence-associated protease secretion in pseudomonas aeruginosa PAO1 |
| title_full_unstemmed | Global regulator morA affects virulence-associated protease secretion in pseudomonas aeruginosa PAO1 |
| title_short | Global regulator morA affects virulence-associated protease secretion in pseudomonas aeruginosa PAO1 |
| title_sort | global regulator mora affects virulence associated protease secretion in pseudomonas aeruginosa pao1 |
| topic | DRNTU::Science::Biological sciences::Microbiology::Bacteria |
| url | https://hdl.handle.net/10356/103501 http://hdl.handle.net/10220/25829 |
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