Twilight, a Novel Circadian-Regulated Gene, Integrates Phototropism with Nutrient and Redox Homeostasis during Fungal Development
Phototropic regulation of circadian clock is important for environmental adaptation, organismal growth and differentiation. Light plays a critical role in fungal development and virulence. However, it is unclear what governs the intracellular metabolic response to such dark-light rhythms in fungi. H...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2015
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| Online Access: | https://hdl.handle.net/10356/103524 http://hdl.handle.net/10220/38767 |
| _version_ | 1826114243050602496 |
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| author | Deng, Yi Zhen Qu, Ziwei Naqvi, Naweed Isaak |
| author2 | Guo, Hui-Shan |
| author_facet | Guo, Hui-Shan Deng, Yi Zhen Qu, Ziwei Naqvi, Naweed Isaak |
| author_sort | Deng, Yi Zhen |
| collection | NTU |
| description | Phototropic regulation of circadian clock is important for environmental adaptation, organismal growth and differentiation. Light plays a critical role in fungal development and virulence. However, it is unclear what governs the intracellular metabolic response to such dark-light rhythms in fungi. Here, we describe a novel circadian-regulated Twilight (TWL) function essential for phototropic induction of asexual development and pathogenesis in the rice-blast fungus Magnaporthe oryzae. The TWL transcript oscillates during circadian cycles and peaks at subjective twilight. GFP-Twl remains acetylated and cytosolic in the dark, whereas light-induced phosphorylation (by the carbon sensor Snf1 kinase) drives it into the nucleus. The mRNA level of the transcription/repair factor TFB5, was significantly down regulated in the twl∆ mutant. Overexpression of TFB5 significantly suppressed the conidiation defects in the twl∆ mutant. Furthermore, Tfb5-GFP translocates to the nucleus during the phototropic response and under redox stress, while it failed to do so in the twl∆ mutant. Thus, we provide mechanistic insight into Twl-based regulation of nutrient and redox homeostasis in response to light during pathogen adaptation to the host milieu in the rice blast pathosystem. |
| first_indexed | 2024-10-01T03:36:18Z |
| format | Journal Article |
| id | ntu-10356/103524 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:36:18Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/1035242023-02-28T16:56:20Z Twilight, a Novel Circadian-Regulated Gene, Integrates Phototropism with Nutrient and Redox Homeostasis during Fungal Development Deng, Yi Zhen Qu, Ziwei Naqvi, Naweed Isaak Guo, Hui-Shan School of Biological Sciences Phototropic regulation of circadian clock is important for environmental adaptation, organismal growth and differentiation. Light plays a critical role in fungal development and virulence. However, it is unclear what governs the intracellular metabolic response to such dark-light rhythms in fungi. Here, we describe a novel circadian-regulated Twilight (TWL) function essential for phototropic induction of asexual development and pathogenesis in the rice-blast fungus Magnaporthe oryzae. The TWL transcript oscillates during circadian cycles and peaks at subjective twilight. GFP-Twl remains acetylated and cytosolic in the dark, whereas light-induced phosphorylation (by the carbon sensor Snf1 kinase) drives it into the nucleus. The mRNA level of the transcription/repair factor TFB5, was significantly down regulated in the twl∆ mutant. Overexpression of TFB5 significantly suppressed the conidiation defects in the twl∆ mutant. Furthermore, Tfb5-GFP translocates to the nucleus during the phototropic response and under redox stress, while it failed to do so in the twl∆ mutant. Thus, we provide mechanistic insight into Twl-based regulation of nutrient and redox homeostasis in response to light during pathogen adaptation to the host milieu in the rice blast pathosystem. Published version 2015-10-01T07:27:04Z 2019-12-06T21:14:32Z 2015-10-01T07:27:04Z 2019-12-06T21:14:32Z 2015 2015 Journal Article Deng, Y. Z., Qu, Z., & Naqvi, N. I. (2015). Twilight, a Novel Circadian-Regulated Gene, Integrates Phototropism with Nutrient and Redox Homeostasis during Fungal Development. PLOS Pathogens, 11(6), e1004972-. 1553-7374 https://hdl.handle.net/10356/103524 http://hdl.handle.net/10220/38767 10.1371/journal.ppat.1004972 26102503 en PLOS Pathogens © 2015 Deng et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. application/pdf |
| spellingShingle | Deng, Yi Zhen Qu, Ziwei Naqvi, Naweed Isaak Twilight, a Novel Circadian-Regulated Gene, Integrates Phototropism with Nutrient and Redox Homeostasis during Fungal Development |
| title | Twilight, a Novel Circadian-Regulated Gene, Integrates Phototropism with Nutrient and Redox Homeostasis during Fungal Development |
| title_full | Twilight, a Novel Circadian-Regulated Gene, Integrates Phototropism with Nutrient and Redox Homeostasis during Fungal Development |
| title_fullStr | Twilight, a Novel Circadian-Regulated Gene, Integrates Phototropism with Nutrient and Redox Homeostasis during Fungal Development |
| title_full_unstemmed | Twilight, a Novel Circadian-Regulated Gene, Integrates Phototropism with Nutrient and Redox Homeostasis during Fungal Development |
| title_short | Twilight, a Novel Circadian-Regulated Gene, Integrates Phototropism with Nutrient and Redox Homeostasis during Fungal Development |
| title_sort | twilight a novel circadian regulated gene integrates phototropism with nutrient and redox homeostasis during fungal development |
| url | https://hdl.handle.net/10356/103524 http://hdl.handle.net/10220/38767 |
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