A new molecular surveillance system for Leishmaniasis
Presently, global efforts are being made to control and eradicate the deadliest tropical diseases through the improvement of adequate interventions. A critical point for programs to succeed is the prompt and accurate diagnosis in endemic regions. Rapid diagnostic tests (RDTs) are being massively dep...
| Main Authors: | , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2014
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/103645 http://hdl.handle.net/10220/20298 |
| _version_ | 1826126475324030976 |
|---|---|
| author | Pandey, Kishor Pandey, Basu Dev Mallik, Arun Kumar Acharya, Jyoti Kato, Kentaro Kaneko, Osamu Ferreira, Pedro Eduardo |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Pandey, Kishor Pandey, Basu Dev Mallik, Arun Kumar Acharya, Jyoti Kato, Kentaro Kaneko, Osamu Ferreira, Pedro Eduardo |
| author_sort | Pandey, Kishor |
| collection | NTU |
| description | Presently, global efforts are being made to control and eradicate the deadliest tropical diseases through the improvement of adequate interventions. A critical point for programs to succeed is the prompt and accurate diagnosis in endemic regions. Rapid diagnostic tests (RDTs) are being massively deployed and used to improve diagnosis in tropical countries. In the present report, we evaluated the hypothesis of, after use for diagnosis, the reuse of the Leishmania RDT kit as a DNA source, which can be used downstream as a molecular surveillance and/or quality control tool. As a proof of principle, a polymerase chain reaction-based method was used to detect Leishmania spp. minicircle kinetoplast DNA from leishmaniasis RDT kits. Our results show that Leishmania spp. DNA can be extracted from used RDTs and may constitute an important, reliable, and affordable tool to assist in future leishmaniasis molecular surveillance methods. |
| first_indexed | 2024-10-01T06:53:16Z |
| format | Journal Article |
| id | ntu-10356/103645 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:53:16Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/1036452023-02-28T17:06:10Z A new molecular surveillance system for Leishmaniasis Pandey, Kishor Pandey, Basu Dev Mallik, Arun Kumar Acharya, Jyoti Kato, Kentaro Kaneko, Osamu Ferreira, Pedro Eduardo School of Biological Sciences DRNTU::Science::Biological sciences Presently, global efforts are being made to control and eradicate the deadliest tropical diseases through the improvement of adequate interventions. A critical point for programs to succeed is the prompt and accurate diagnosis in endemic regions. Rapid diagnostic tests (RDTs) are being massively deployed and used to improve diagnosis in tropical countries. In the present report, we evaluated the hypothesis of, after use for diagnosis, the reuse of the Leishmania RDT kit as a DNA source, which can be used downstream as a molecular surveillance and/or quality control tool. As a proof of principle, a polymerase chain reaction-based method was used to detect Leishmania spp. minicircle kinetoplast DNA from leishmaniasis RDT kits. Our results show that Leishmania spp. DNA can be extracted from used RDTs and may constitute an important, reliable, and affordable tool to assist in future leishmaniasis molecular surveillance methods. Published version 2014-08-15T05:51:51Z 2019-12-06T21:16:59Z 2014-08-15T05:51:51Z 2019-12-06T21:16:59Z 2014 2014 Journal Article Pandey, K., Pandey, B. D., Mallik, A. K., Acharya, J., Kato, K., Kaneko, O., et al. (2014). A New Molecular Surveillance System for Leishmaniasis. American Journal of Tropical Medicine and Hygiene, 13-0329. 0002-9637 https://hdl.handle.net/10356/103645 http://hdl.handle.net/10220/20298 10.4269/ajtmh.13-0329 24752687 178903 en American journal of tropical medicine and hygiene © 2014 American Society of Tropical Medicine and Hygiene. This paper was published in American Journal of Tropical Medicine and Hygiene and is made available as an electronic reprint (preprint) with permission of American Society of Tropical Medicine and Hygiene. The paper can be found at the following official DOI: [http://dx.doi.org/10.4269/ajtmh.13-0329]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Pandey, Kishor Pandey, Basu Dev Mallik, Arun Kumar Acharya, Jyoti Kato, Kentaro Kaneko, Osamu Ferreira, Pedro Eduardo A new molecular surveillance system for Leishmaniasis |
| title | A new molecular surveillance system for Leishmaniasis |
| title_full | A new molecular surveillance system for Leishmaniasis |
| title_fullStr | A new molecular surveillance system for Leishmaniasis |
| title_full_unstemmed | A new molecular surveillance system for Leishmaniasis |
| title_short | A new molecular surveillance system for Leishmaniasis |
| title_sort | new molecular surveillance system for leishmaniasis |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/103645 http://hdl.handle.net/10220/20298 |
| work_keys_str_mv | AT pandeykishor anewmolecularsurveillancesystemforleishmaniasis AT pandeybasudev anewmolecularsurveillancesystemforleishmaniasis AT mallikarunkumar anewmolecularsurveillancesystemforleishmaniasis AT acharyajyoti anewmolecularsurveillancesystemforleishmaniasis AT katokentaro anewmolecularsurveillancesystemforleishmaniasis AT kanekoosamu anewmolecularsurveillancesystemforleishmaniasis AT ferreirapedroeduardo anewmolecularsurveillancesystemforleishmaniasis AT pandeykishor newmolecularsurveillancesystemforleishmaniasis AT pandeybasudev newmolecularsurveillancesystemforleishmaniasis AT mallikarunkumar newmolecularsurveillancesystemforleishmaniasis AT acharyajyoti newmolecularsurveillancesystemforleishmaniasis AT katokentaro newmolecularsurveillancesystemforleishmaniasis AT kanekoosamu newmolecularsurveillancesystemforleishmaniasis AT ferreirapedroeduardo newmolecularsurveillancesystemforleishmaniasis |