A generic micropatterning platform to direct human mesenchymal stem cells from different origins towards myogenic differentiation
Human mesenchymal stem cells (MSCs) derived from various origins show varied differentiation capability. Recent work shows that cell shape manipulation via micropatterning can modulate the differentiation of bone-marrow-derived MSCs. Herein, the effect of micropatterning on the myogenesis of MSCs is...
| Main Authors: | , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/103661 http://hdl.handle.net/10220/16726 |
| _version_ | 1824455609605423104 |
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| author | Tan, Lay Poh Yu, Ting Chua, Chee Kai Tay, Chor Yong Wen, Feng Yu, Haiyang Chan, Jerry Kok Yen Chong, Mark Seow Khoon Leong, David Tai Wei |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Tan, Lay Poh Yu, Ting Chua, Chee Kai Tay, Chor Yong Wen, Feng Yu, Haiyang Chan, Jerry Kok Yen Chong, Mark Seow Khoon Leong, David Tai Wei |
| author_sort | Tan, Lay Poh |
| collection | NTU |
| description | Human mesenchymal stem cells (MSCs) derived from various origins show varied differentiation capability. Recent work shows that cell shape manipulation via micropatterning can modulate the differentiation of bone-marrow-derived MSCs. Herein, the effect of micropatterning on the myogenesis of MSCs isolated from three different sources (bone marrow, fetal tissue, and adipose) is reported. All the well-aligned cells, regardless of source, predominantly commit to myogenic lineage, as shown by the significant upregulation of myogenic gene markers and positive myosin heavy chain staining. It is demonstrated that our novel micropattern can be used as a generic platform for inducing myogenesis of MSCs from different sources and may also have the potential to be extended to induce other lineage commitment. |
| first_indexed | 2025-02-19T03:40:56Z |
| format | Journal Article |
| id | ntu-10356/103661 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:40:56Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/1036612020-06-01T10:13:38Z A generic micropatterning platform to direct human mesenchymal stem cells from different origins towards myogenic differentiation Tan, Lay Poh Yu, Ting Chua, Chee Kai Tay, Chor Yong Wen, Feng Yu, Haiyang Chan, Jerry Kok Yen Chong, Mark Seow Khoon Leong, David Tai Wei School of Chemical and Biomedical Engineering School of Materials Science & Engineering School of Mechanical and Aerospace Engineering DRNTU::Engineering::Chemical engineering::Biochemical engineering Human mesenchymal stem cells (MSCs) derived from various origins show varied differentiation capability. Recent work shows that cell shape manipulation via micropatterning can modulate the differentiation of bone-marrow-derived MSCs. Herein, the effect of micropatterning on the myogenesis of MSCs isolated from three different sources (bone marrow, fetal tissue, and adipose) is reported. All the well-aligned cells, regardless of source, predominantly commit to myogenic lineage, as shown by the significant upregulation of myogenic gene markers and positive myosin heavy chain staining. It is demonstrated that our novel micropattern can be used as a generic platform for inducing myogenesis of MSCs from different sources and may also have the potential to be extended to induce other lineage commitment. 2013-10-23T07:03:10Z 2019-12-06T21:17:16Z 2013-10-23T07:03:10Z 2019-12-06T21:17:16Z 2013 2013 Journal Article Yu, T., Chua, C. K., Tay, C. Y., Wen, F., Yu, H., Chan, J. K. Y., et al. (2013). A generic micropatterning platform to direct human mesenchymal stem cells from different origins towards myogenic differentiation. Macromolecular bioscience, 13(6), 799-807. 1616-5187 https://hdl.handle.net/10356/103661 http://hdl.handle.net/10220/16726 10.1002/mabi.201200481 en Macromolecular bioscience |
| spellingShingle | DRNTU::Engineering::Chemical engineering::Biochemical engineering Tan, Lay Poh Yu, Ting Chua, Chee Kai Tay, Chor Yong Wen, Feng Yu, Haiyang Chan, Jerry Kok Yen Chong, Mark Seow Khoon Leong, David Tai Wei A generic micropatterning platform to direct human mesenchymal stem cells from different origins towards myogenic differentiation |
| title | A generic micropatterning platform to direct human mesenchymal stem cells from different origins towards myogenic differentiation |
| title_full | A generic micropatterning platform to direct human mesenchymal stem cells from different origins towards myogenic differentiation |
| title_fullStr | A generic micropatterning platform to direct human mesenchymal stem cells from different origins towards myogenic differentiation |
| title_full_unstemmed | A generic micropatterning platform to direct human mesenchymal stem cells from different origins towards myogenic differentiation |
| title_short | A generic micropatterning platform to direct human mesenchymal stem cells from different origins towards myogenic differentiation |
| title_sort | generic micropatterning platform to direct human mesenchymal stem cells from different origins towards myogenic differentiation |
| topic | DRNTU::Engineering::Chemical engineering::Biochemical engineering |
| url | https://hdl.handle.net/10356/103661 http://hdl.handle.net/10220/16726 |
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