A novel λ integrase-mediated seamless vector transgenesis platform for therapeutic protein expression
Advances in stem cell engineering, gene therapy and molecular medicine often involve genome engineering at a cellular level. However, functionally large or multi transgene cassette insertion into the human genome still remains a challenge. Current practices such as random transgene integration or ta...
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| Format: | Journal Article |
| Language: | English |
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2019
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| Online Access: | https://hdl.handle.net/10356/103690 http://hdl.handle.net/10220/47376 |
| _version_ | 1826127479114301440 |
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| author | Makhija, Harshyaa Roy, Suki Hoon, Shawn Ghadessy, Farid John Wong, Desmond Jaiswal, Rahul Campana, Dario Dröge, Peter |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Makhija, Harshyaa Roy, Suki Hoon, Shawn Ghadessy, Farid John Wong, Desmond Jaiswal, Rahul Campana, Dario Dröge, Peter |
| author_sort | Makhija, Harshyaa |
| collection | NTU |
| description | Advances in stem cell engineering, gene therapy and molecular medicine often involve genome engineering at a cellular level. However, functionally large or multi transgene cassette insertion into the human genome still remains a challenge. Current practices such as random transgene integration or targeted endonuclease-based genome editing are suboptimal and might pose safety concerns. Taking this into consideration, we previously developed a transgenesis tool derived from phage λ integrase (Int) that precisely recombines large plasmid DNA into an endogenous sequence found in human Long INterspersed Elements-1 (LINE-1). Despite this advancement, biosafety concerns associated with bacterial components of plasmids, enhanced uptake and efficient transgene expression remained problematic. We therefore further improved and herein report a more superior Int-based transgenesis tool. This novel Int platform allows efficient and easy derivation of sufficient amounts of seamless supercoiled transgene vectors from conventional plasmids via intramolecular recombination as well as subsequent intermolecular site-specific genome integration into LINE-1. Furthermore, we identified certain LINE-1 as preferred insertion sites for Int-mediated seamless vector transgenesis, and showed that targeted anti-CD19 chimeric antigen receptor gene integration achieves high-level sustained transgene expression in human embryonic stem cell clones for potential downstream therapeutic applications. |
| first_indexed | 2024-10-01T07:09:33Z |
| format | Journal Article |
| id | ntu-10356/103690 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:09:33Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/1036902023-02-28T17:06:11Z A novel λ integrase-mediated seamless vector transgenesis platform for therapeutic protein expression Makhija, Harshyaa Roy, Suki Hoon, Shawn Ghadessy, Farid John Wong, Desmond Jaiswal, Rahul Campana, Dario Dröge, Peter School of Biological Sciences NTU Institute of Structural Biology Transgenesis Protein Expression DRNTU::Science::Biological sciences Advances in stem cell engineering, gene therapy and molecular medicine often involve genome engineering at a cellular level. However, functionally large or multi transgene cassette insertion into the human genome still remains a challenge. Current practices such as random transgene integration or targeted endonuclease-based genome editing are suboptimal and might pose safety concerns. Taking this into consideration, we previously developed a transgenesis tool derived from phage λ integrase (Int) that precisely recombines large plasmid DNA into an endogenous sequence found in human Long INterspersed Elements-1 (LINE-1). Despite this advancement, biosafety concerns associated with bacterial components of plasmids, enhanced uptake and efficient transgene expression remained problematic. We therefore further improved and herein report a more superior Int-based transgenesis tool. This novel Int platform allows efficient and easy derivation of sufficient amounts of seamless supercoiled transgene vectors from conventional plasmids via intramolecular recombination as well as subsequent intermolecular site-specific genome integration into LINE-1. Furthermore, we identified certain LINE-1 as preferred insertion sites for Int-mediated seamless vector transgenesis, and showed that targeted anti-CD19 chimeric antigen receptor gene integration achieves high-level sustained transgene expression in human embryonic stem cell clones for potential downstream therapeutic applications. ASTAR (Agency for Sci., Tech. and Research, S’pore) NMRC (Natl Medical Research Council, S’pore) Published version 2019-01-04T07:50:17Z 2019-12-06T21:18:03Z 2019-01-04T07:50:17Z 2019-12-06T21:18:03Z 2018 Journal Article Makhija, H., Roy, S., Hoon, S., Ghadessy, F. J., Wong, D., Jaiswal, R., . . . Dröge, P. (2018). A novel λ integrase-mediated seamless vector transgenesis platform for therapeutic protein expression. Nucleic Acids Research, 46(16), e99-. doi:10.1093/nar/gky500 0305-1048 https://hdl.handle.net/10356/103690 http://hdl.handle.net/10220/47376 10.1093/nar/gky500 en Nucleic Acids Research © 2018 The Author(s). Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com 14 p. application/pdf |
| spellingShingle | Transgenesis Protein Expression DRNTU::Science::Biological sciences Makhija, Harshyaa Roy, Suki Hoon, Shawn Ghadessy, Farid John Wong, Desmond Jaiswal, Rahul Campana, Dario Dröge, Peter A novel λ integrase-mediated seamless vector transgenesis platform for therapeutic protein expression |
| title | A novel λ integrase-mediated seamless vector transgenesis platform for therapeutic protein expression |
| title_full | A novel λ integrase-mediated seamless vector transgenesis platform for therapeutic protein expression |
| title_fullStr | A novel λ integrase-mediated seamless vector transgenesis platform for therapeutic protein expression |
| title_full_unstemmed | A novel λ integrase-mediated seamless vector transgenesis platform for therapeutic protein expression |
| title_short | A novel λ integrase-mediated seamless vector transgenesis platform for therapeutic protein expression |
| title_sort | novel λ integrase mediated seamless vector transgenesis platform for therapeutic protein expression |
| topic | Transgenesis Protein Expression DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/103690 http://hdl.handle.net/10220/47376 |
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