Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering
Introduction: In search for cross-linkers with multifunctional characteristics, the present work investigated the utility of quaternary ammonium organosilane (QOS) as a potential cross-linker for electrospun collagen nanofibers. We hypothesized that the quaternary ammonium ions improve the electrosp...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2019
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/103718 http://hdl.handle.net/10220/47368 |
| _version_ | 1826117548515524608 |
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| author | Dhand, Chetna Balakrishnan, Yamini Ong, Seow Theng Dwivedi, Neeraj Venugopal, Jayarama R. Harini, Sriram Leung, Chak Ming Low, Kenny Zhi Wei Loh, Xian Jun Beuerman, Roger W. Ramakrishna, Seeram Verma, Navin Kumar Lakshminarayanan, Rajamani |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Dhand, Chetna Balakrishnan, Yamini Ong, Seow Theng Dwivedi, Neeraj Venugopal, Jayarama R. Harini, Sriram Leung, Chak Ming Low, Kenny Zhi Wei Loh, Xian Jun Beuerman, Roger W. Ramakrishna, Seeram Verma, Navin Kumar Lakshminarayanan, Rajamani |
| author_sort | Dhand, Chetna |
| collection | NTU |
| description | Introduction: In search for cross-linkers with multifunctional characteristics, the present work investigated the utility of quaternary ammonium organosilane (QOS) as a potential cross-linker for electrospun collagen nanofibers. We hypothesized that the quaternary ammonium ions improve the electrospinnability by reducing the surface tension and confer antimicrobial properties, while the formation of siloxane after alkaline hydrolysis could cross-link collagen and stimulate cell proliferation. Materials and methods: QOS collagen nanofibers were electrospun by incorporating various concentrations of QOS (0.1%–10% w/w) and were cross-linked in situ after exposure to ammonium carbonate. The QOS cross-linked scaffolds were characterized and their biological properties were evaluated in terms of their biocompatibility, cellular adhesion and metabolic activity for primary human dermal fibroblasts and human fetal osteoblasts. Results and discussion: The study revealed that 1) QOS cross-linking increased the flexibility of otherwise rigid collagen nanofibers and improved the thermal stability; 2) QOS cross-linked mats displayed potent antibacterial activity and 3) the biocompatibility of the composite mats depended on the amount of QOS present in dope solution – at low QOS concentrations (0.1% w/w), the mats promoted mammalian cell proliferation and growth, whereas at higher QOS concentrations, cytotoxic effect was observed. Conclusion: This study demonstrates that QOS cross-linked mats possess anti-infective properties and confer niches for cellular growth and proliferation, thus offering a useful approach, which is important for hard and soft tissue engineering and regenerative medicine. |
| first_indexed | 2024-10-01T04:29:04Z |
| format | Journal Article |
| id | ntu-10356/103718 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:29:04Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/1037182020-11-01T05:18:05Z Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering Dhand, Chetna Balakrishnan, Yamini Ong, Seow Theng Dwivedi, Neeraj Venugopal, Jayarama R. Harini, Sriram Leung, Chak Ming Low, Kenny Zhi Wei Loh, Xian Jun Beuerman, Roger W. Ramakrishna, Seeram Verma, Navin Kumar Lakshminarayanan, Rajamani Lee Kong Chian School of Medicine (LKCMedicine) DRNTU::Science::Medicine Anti-infective Wound Dressing Cyto-compatible Nanofibre Introduction: In search for cross-linkers with multifunctional characteristics, the present work investigated the utility of quaternary ammonium organosilane (QOS) as a potential cross-linker for electrospun collagen nanofibers. We hypothesized that the quaternary ammonium ions improve the electrospinnability by reducing the surface tension and confer antimicrobial properties, while the formation of siloxane after alkaline hydrolysis could cross-link collagen and stimulate cell proliferation. Materials and methods: QOS collagen nanofibers were electrospun by incorporating various concentrations of QOS (0.1%–10% w/w) and were cross-linked in situ after exposure to ammonium carbonate. The QOS cross-linked scaffolds were characterized and their biological properties were evaluated in terms of their biocompatibility, cellular adhesion and metabolic activity for primary human dermal fibroblasts and human fetal osteoblasts. Results and discussion: The study revealed that 1) QOS cross-linking increased the flexibility of otherwise rigid collagen nanofibers and improved the thermal stability; 2) QOS cross-linked mats displayed potent antibacterial activity and 3) the biocompatibility of the composite mats depended on the amount of QOS present in dope solution – at low QOS concentrations (0.1% w/w), the mats promoted mammalian cell proliferation and growth, whereas at higher QOS concentrations, cytotoxic effect was observed. Conclusion: This study demonstrates that QOS cross-linked mats possess anti-infective properties and confer niches for cellular growth and proliferation, thus offering a useful approach, which is important for hard and soft tissue engineering and regenerative medicine. MOE (Min. of Education, S’pore) NMRC (Natl Medical Research Council, S’pore) MOH (Min. of Health, S’pore) Published version 2019-01-04T05:20:06Z 2019-12-06T21:18:43Z 2019-01-04T05:20:06Z 2019-12-06T21:18:43Z 2018 Journal Article Dhand, C., Balakrishnan, Y., Ong, S. T., Dwivedi, N., Venugopal, J. R., Harini, S., ... Lakshminarayanan, R. (2018). Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering. International Journal of Nanomedicine, Volume 13, 4473-4492. doi:10.2147/IJN.S159770 1176-9114 https://hdl.handle.net/10356/103718 http://hdl.handle.net/10220/47368 10.2147/IJN.S159770 en International Journal of Nanomedicine © 2018 Dhand et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). 20 p. application/pdf |
| spellingShingle | DRNTU::Science::Medicine Anti-infective Wound Dressing Cyto-compatible Nanofibre Dhand, Chetna Balakrishnan, Yamini Ong, Seow Theng Dwivedi, Neeraj Venugopal, Jayarama R. Harini, Sriram Leung, Chak Ming Low, Kenny Zhi Wei Loh, Xian Jun Beuerman, Roger W. Ramakrishna, Seeram Verma, Navin Kumar Lakshminarayanan, Rajamani Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering |
| title | Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering |
| title_full | Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering |
| title_fullStr | Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering |
| title_full_unstemmed | Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering |
| title_short | Antimicrobial quaternary ammonium organosilane cross-linked nanofibrous collagen scaffolds for tissue engineering |
| title_sort | antimicrobial quaternary ammonium organosilane cross linked nanofibrous collagen scaffolds for tissue engineering |
| topic | DRNTU::Science::Medicine Anti-infective Wound Dressing Cyto-compatible Nanofibre |
| url | https://hdl.handle.net/10356/103718 http://hdl.handle.net/10220/47368 |
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