Transcriptome analysis identified genes for growth and omega-3/-6 ratio in saline tilapia
Growth and omega-3/-6 ratio are important traits in aquaculture. The mechanisms underlying quick growth and high omega-3/-6 ratio in fish are not fully understood. The consumption of the meat of tilapia suffers a bad reputation due to its low omega-3/-6 ratio. To facilitate the improvement of these...
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2019
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| Online Access: | https://hdl.handle.net/10356/103978 http://hdl.handle.net/10220/48626 |
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| author | Lin, Grace Thevasagayam, Natascha M. Wan, Zi Yi Ye, B. Q. Yue, Gen Hua |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Lin, Grace Thevasagayam, Natascha M. Wan, Zi Yi Ye, B. Q. Yue, Gen Hua |
| author_sort | Lin, Grace |
| collection | NTU |
| description | Growth and omega-3/-6 ratio are important traits in aquaculture. The mechanisms underlying quick growth and high omega-3/-6 ratio in fish are not fully understood. The consumption of the meat of tilapia suffers a bad reputation due to its low omega-3/-6 ratio. To facilitate the improvement of these traits and to understand more about the mechanisms underlying quick growth and high omega-3/-6 ratio, we conducted transcriptome analysis in the muscle and liver of fast- and slow-growing hybrid saline tilapia generated by crossing Mozambique tilapia and red tilapia. A transcriptome with an average length of 963 bp was generated by using 486.65 million clean 100 bp paired-end reads. A total of 42,699 annotated unique sequences with an average length of 3.4 kb were obtained. Differentially expressed genes (DEGs) in the muscle and liver were identified between fast- and slow-growing tilapia. Pathway analysis classified these genes into many pathways. Ten genes, including foxK1, sparc, smad3, usp38, crot, fadps, sqlea, cyp7b1, impa1, and gss, from the DEGs were located within QTL for growth and omega-3, which were previously detected content in tilapia, suggesting that these ten genes could be important candidate genes for growth and omega-3 fatty acid content. Analysis of SNPs in introns 1 and 2 of foxK1 revealed that the SNPs were significantly associated with growth and omega-3/-6 ratio. This study lays the groundwork for further investigation of the molecular mechanisms underlying the phenotypic variation of these two traits and provides SNPs for selecting these traits at fingerling stage. |
| first_indexed | 2024-10-01T04:58:46Z |
| format | Journal Article |
| id | ntu-10356/103978 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:58:46Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/1039782023-02-28T17:05:28Z Transcriptome analysis identified genes for growth and omega-3/-6 ratio in saline tilapia Lin, Grace Thevasagayam, Natascha M. Wan, Zi Yi Ye, B. Q. Yue, Gen Hua School of Biological Sciences Growth DRNTU::Science::Biological sciences Tilapia Growth and omega-3/-6 ratio are important traits in aquaculture. The mechanisms underlying quick growth and high omega-3/-6 ratio in fish are not fully understood. The consumption of the meat of tilapia suffers a bad reputation due to its low omega-3/-6 ratio. To facilitate the improvement of these traits and to understand more about the mechanisms underlying quick growth and high omega-3/-6 ratio, we conducted transcriptome analysis in the muscle and liver of fast- and slow-growing hybrid saline tilapia generated by crossing Mozambique tilapia and red tilapia. A transcriptome with an average length of 963 bp was generated by using 486.65 million clean 100 bp paired-end reads. A total of 42,699 annotated unique sequences with an average length of 3.4 kb were obtained. Differentially expressed genes (DEGs) in the muscle and liver were identified between fast- and slow-growing tilapia. Pathway analysis classified these genes into many pathways. Ten genes, including foxK1, sparc, smad3, usp38, crot, fadps, sqlea, cyp7b1, impa1, and gss, from the DEGs were located within QTL for growth and omega-3, which were previously detected content in tilapia, suggesting that these ten genes could be important candidate genes for growth and omega-3 fatty acid content. Analysis of SNPs in introns 1 and 2 of foxK1 revealed that the SNPs were significantly associated with growth and omega-3/-6 ratio. This study lays the groundwork for further investigation of the molecular mechanisms underlying the phenotypic variation of these two traits and provides SNPs for selecting these traits at fingerling stage. NRF (Natl Research Foundation, S’pore) Published version 2019-06-11T04:14:28Z 2019-12-06T21:23:53Z 2019-06-11T04:14:28Z 2019-12-06T21:23:53Z 2019 Journal Article Lin, G., Thevasagayam, N. M., Wan, Z. Y., Ye, B. Q., & Yue, G. H. (2019). Transcriptome analysis identified genes for growth and omega-3/-6 ratio in saline tilapia. Frontiers in Genetics, 10, 244-. doi:10.3389/fgene.2019.00244 https://hdl.handle.net/10356/103978 http://hdl.handle.net/10220/48626 10.3389/fgene.2019.00244 en Frontiers in Genetics © 2019 Lin, Thevasagayam, Wan, Ye and Yue. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. 11 p. application/pdf |
| spellingShingle | Growth DRNTU::Science::Biological sciences Tilapia Lin, Grace Thevasagayam, Natascha M. Wan, Zi Yi Ye, B. Q. Yue, Gen Hua Transcriptome analysis identified genes for growth and omega-3/-6 ratio in saline tilapia |
| title | Transcriptome analysis identified genes for growth and omega-3/-6 ratio in saline tilapia |
| title_full | Transcriptome analysis identified genes for growth and omega-3/-6 ratio in saline tilapia |
| title_fullStr | Transcriptome analysis identified genes for growth and omega-3/-6 ratio in saline tilapia |
| title_full_unstemmed | Transcriptome analysis identified genes for growth and omega-3/-6 ratio in saline tilapia |
| title_short | Transcriptome analysis identified genes for growth and omega-3/-6 ratio in saline tilapia |
| title_sort | transcriptome analysis identified genes for growth and omega 3 6 ratio in saline tilapia |
| topic | Growth DRNTU::Science::Biological sciences Tilapia |
| url | https://hdl.handle.net/10356/103978 http://hdl.handle.net/10220/48626 |
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