Proteomics based identification of cell migration related proteins in HBV expressing HepG2 cells
Proteomics study was performed to investigate the specific protein expression profiles of HepG2 cells transfected with mutant HBV compared with wildtype HBV genome, aiming to identify the specific functions of SH3 binding domain (proline rich region) located in HBx. In addition to the cell movement...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2014
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| Online Access: | https://hdl.handle.net/10356/104202 http://hdl.handle.net/10220/19560 |
| _version_ | 1826128102119440384 |
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| author | Feng, Huixing Li, Xi Chan, Vincent Chen, Wei Ning |
| author2 | Kaushik-Basu, Neerja |
| author_facet | Kaushik-Basu, Neerja Feng, Huixing Li, Xi Chan, Vincent Chen, Wei Ning |
| author_sort | Feng, Huixing |
| collection | NTU |
| description | Proteomics study was performed to investigate the specific protein expression profiles of HepG2 cells transfected with mutant HBV compared with wildtype HBV genome, aiming to identify the specific functions of SH3 binding domain (proline rich region) located in HBx. In addition to the cell movement and kinetics changes due to the expression of HBV genome we have observed previously, here we further targeted to explore the specific changes of cellular proteins and potential intracellular protein interactions, which might provide more information of the potential cellular mechanism of the differentiated cell movements. Specific changes of a number of proteins were shown in global protein profiling in HepG2 cells expressing wildtype HBV, including cell migration related proteins, and interestingly the changes were found recovered by SH3 binding domain mutated HBV. The distinctive expressions of proteins were validated by Western blot analysis. |
| first_indexed | 2024-10-01T07:19:25Z |
| format | Journal Article |
| id | ntu-10356/104202 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:19:25Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/1042022023-12-29T06:52:03Z Proteomics based identification of cell migration related proteins in HBV expressing HepG2 cells Feng, Huixing Li, Xi Chan, Vincent Chen, Wei Ning Kaushik-Basu, Neerja School of Chemical and Biomedical Engineering DRNTU::Science::Biological sciences::Biochemistry Proteomics study was performed to investigate the specific protein expression profiles of HepG2 cells transfected with mutant HBV compared with wildtype HBV genome, aiming to identify the specific functions of SH3 binding domain (proline rich region) located in HBx. In addition to the cell movement and kinetics changes due to the expression of HBV genome we have observed previously, here we further targeted to explore the specific changes of cellular proteins and potential intracellular protein interactions, which might provide more information of the potential cellular mechanism of the differentiated cell movements. Specific changes of a number of proteins were shown in global protein profiling in HepG2 cells expressing wildtype HBV, including cell migration related proteins, and interestingly the changes were found recovered by SH3 binding domain mutated HBV. The distinctive expressions of proteins were validated by Western blot analysis. Published version 2014-06-04T04:49:23Z 2019-12-06T21:28:24Z 2014-06-04T04:49:23Z 2019-12-06T21:28:24Z 2014 2014 Journal Article Feng, H., Li, X., Chan, V., & Chen, W. N. (2014). Proteomics Based Identification of Cell Migration Related Proteins in HBV Expressing HepG2 Cells. PLoS ONE, 9(4), e95621-. 1932-6203 https://hdl.handle.net/10356/104202 http://hdl.handle.net/10220/19560 10.1371/journal.pone.0095621 24763314 en PLoS ONE © 2014 Feng et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences::Biochemistry Feng, Huixing Li, Xi Chan, Vincent Chen, Wei Ning Proteomics based identification of cell migration related proteins in HBV expressing HepG2 cells |
| title | Proteomics based identification of cell migration related proteins in HBV expressing HepG2 cells |
| title_full | Proteomics based identification of cell migration related proteins in HBV expressing HepG2 cells |
| title_fullStr | Proteomics based identification of cell migration related proteins in HBV expressing HepG2 cells |
| title_full_unstemmed | Proteomics based identification of cell migration related proteins in HBV expressing HepG2 cells |
| title_short | Proteomics based identification of cell migration related proteins in HBV expressing HepG2 cells |
| title_sort | proteomics based identification of cell migration related proteins in hbv expressing hepg2 cells |
| topic | DRNTU::Science::Biological sciences::Biochemistry |
| url | https://hdl.handle.net/10356/104202 http://hdl.handle.net/10220/19560 |
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