Organ-on-e-chip : three-dimensional self-rolled biosensor array for electrical interrogations of human electrogenic spheroids
Cell-cell communication plays a pivotal role in coordination and function of biological systems. Three-dimensional (3D) spheroids provide venues to explore cellular communication for tissue development and drug discovery, as their 3D architecture mimics native in vivo microenvironments. Cellular ele...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2019
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| Online Access: | https://hdl.handle.net/10356/104260 http://hdl.handle.net/10220/49992 |
| _version_ | 1826120945039835136 |
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| author | Kalmykov, Anna Huang, Changjin Bliley, Jacqueline Shiwarski, Daniel Tashman, Joshua Rastogi, Sahil K. Shukla, Shivani Mataev, Elnatan Feinberg, Adam W. Hsia, K. Jimmy Cohen-Karni, Tzahi Arif Abdullah |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Kalmykov, Anna Huang, Changjin Bliley, Jacqueline Shiwarski, Daniel Tashman, Joshua Rastogi, Sahil K. Shukla, Shivani Mataev, Elnatan Feinberg, Adam W. Hsia, K. Jimmy Cohen-Karni, Tzahi Arif Abdullah |
| author_sort | Kalmykov, Anna |
| collection | NTU |
| description | Cell-cell communication plays a pivotal role in coordination and function of biological systems. Three-dimensional (3D) spheroids provide venues to explore cellular communication for tissue development and drug discovery, as their 3D architecture mimics native in vivo microenvironments. Cellular electrophysiology is a prevalent signaling paradigm for studying electroactive cells. Currently, electrophysiological studies do not provide direct, multisite, simultaneous investigation of tissues in 3D. In this study, 3D self-rolled biosensor arrays (3D-SR-BAs) of either active field-effect transistors or passive microelectrodes were implemented to interface human cardiac spheroids in 3D. The arrays provided continuous and stable multiplexed recordings of field potentials with high sensitivity and spatiotemporal resolution, supported with simultaneous calcium imaging. Our approach enables electrophysiological investigation and monitoring of the complex signal transduction in 3D cellular assemblies toward an organ-on-an-electronic-chip (organ-on-e-chip) platform for tissue maturation investigations and development of drugs for disease treatment, such as arrhythmias. |
| first_indexed | 2024-10-01T05:24:43Z |
| format | Journal Article |
| id | ntu-10356/104260 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:24:43Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/1042602023-03-04T17:20:39Z Organ-on-e-chip : three-dimensional self-rolled biosensor array for electrical interrogations of human electrogenic spheroids Kalmykov, Anna Huang, Changjin Bliley, Jacqueline Shiwarski, Daniel Tashman, Joshua Rastogi, Sahil K. Shukla, Shivani Mataev, Elnatan Feinberg, Adam W. Hsia, K. Jimmy Cohen-Karni, Tzahi Arif Abdullah School of Chemical and Biomedical Engineering School of Mechanical and Aerospace Engineering Electrical Science::Biological sciences Spheroids Cell-cell communication plays a pivotal role in coordination and function of biological systems. Three-dimensional (3D) spheroids provide venues to explore cellular communication for tissue development and drug discovery, as their 3D architecture mimics native in vivo microenvironments. Cellular electrophysiology is a prevalent signaling paradigm for studying electroactive cells. Currently, electrophysiological studies do not provide direct, multisite, simultaneous investigation of tissues in 3D. In this study, 3D self-rolled biosensor arrays (3D-SR-BAs) of either active field-effect transistors or passive microelectrodes were implemented to interface human cardiac spheroids in 3D. The arrays provided continuous and stable multiplexed recordings of field potentials with high sensitivity and spatiotemporal resolution, supported with simultaneous calcium imaging. Our approach enables electrophysiological investigation and monitoring of the complex signal transduction in 3D cellular assemblies toward an organ-on-an-electronic-chip (organ-on-e-chip) platform for tissue maturation investigations and development of drugs for disease treatment, such as arrhythmias. Published version 2019-09-24T09:15:04Z 2019-12-06T21:29:16Z 2019-09-24T09:15:04Z 2019-12-06T21:29:16Z 2019 Journal Article Kalmykov, A., Huang, C., Bliley, J., Shiwarski, D., Tashman, J., Arif Abdullah, . . . Cohen-Karni, T. (2019). Organ-on-e-chip : three-dimensional self-rolled biosensor array for electrical interrogations of human electrogenic spheroids. Science Advances, 5(8), eaax0729-. doi:10.1126/sciadv.aax0729 https://hdl.handle.net/10356/104260 http://hdl.handle.net/10220/49992 10.1126/sciadv.aax0729 en Science Advances © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). 11 p. application/pdf |
| spellingShingle | Electrical Science::Biological sciences Spheroids Kalmykov, Anna Huang, Changjin Bliley, Jacqueline Shiwarski, Daniel Tashman, Joshua Rastogi, Sahil K. Shukla, Shivani Mataev, Elnatan Feinberg, Adam W. Hsia, K. Jimmy Cohen-Karni, Tzahi Arif Abdullah Organ-on-e-chip : three-dimensional self-rolled biosensor array for electrical interrogations of human electrogenic spheroids |
| title | Organ-on-e-chip : three-dimensional self-rolled biosensor array for electrical interrogations of human electrogenic spheroids |
| title_full | Organ-on-e-chip : three-dimensional self-rolled biosensor array for electrical interrogations of human electrogenic spheroids |
| title_fullStr | Organ-on-e-chip : three-dimensional self-rolled biosensor array for electrical interrogations of human electrogenic spheroids |
| title_full_unstemmed | Organ-on-e-chip : three-dimensional self-rolled biosensor array for electrical interrogations of human electrogenic spheroids |
| title_short | Organ-on-e-chip : three-dimensional self-rolled biosensor array for electrical interrogations of human electrogenic spheroids |
| title_sort | organ on e chip three dimensional self rolled biosensor array for electrical interrogations of human electrogenic spheroids |
| topic | Electrical Science::Biological sciences Spheroids |
| url | https://hdl.handle.net/10356/104260 http://hdl.handle.net/10220/49992 |
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