Microtubule-like properties of the bacterial actin homolog ParM-R1
In preparation for mammalian cell division, microtubules repeatedly probe the cytoplasm to capture chromosomes and assemble the mitotic spindle. Critical features of this microtubule system are the formation of radial arrays centered at the centrosomes and dynamic instability, leading to persistent...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/104888 http://hdl.handle.net/10220/17016 |
| _version_ | 1826124916155482112 |
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| author | Popp, David. Narita, Akihiro. Lee, Lin Jie. Larsson, Mårten. Robinson, Robert C. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Popp, David. Narita, Akihiro. Lee, Lin Jie. Larsson, Mårten. Robinson, Robert C. |
| author_sort | Popp, David. |
| collection | NTU |
| description | In preparation for mammalian cell division, microtubules repeatedly probe the cytoplasm to capture chromosomes and assemble the mitotic spindle. Critical features of this microtubule system are the formation of radial arrays centered at the centrosomes and dynamic instability, leading to persistent cycles of polymerization and depolymerization. Here, we show that actin homolog, ParM-R1 that drives segregation of the R1 multidrug resistance plasmid from Escherichia coli, can also self-organize in vitro into asters, which resemble astral microtubules. ParM-R1 asters grow from centrosome-like structures consisting of interconnected nodes related by a pseudo 8-fold symmetry. In addition, we show that ParM-R1 is able to perform persistent microtubule-like oscillations of assembly and disassembly. In vitro, a whole population of ParM-R1 filaments is synchronized between phases of growth and shrinkage, leading to prolonged synchronous oscillations even at physiological ParM-R1 concentrations. These results imply that the selection pressure to reliably segregate DNA during cell division has led to common mechanisms within diverse segregation machineries. |
| first_indexed | 2024-10-01T06:28:11Z |
| format | Journal Article |
| id | ntu-10356/104888 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:28:11Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/1048882022-02-16T16:27:19Z Microtubule-like properties of the bacterial actin homolog ParM-R1 Popp, David. Narita, Akihiro. Lee, Lin Jie. Larsson, Mårten. Robinson, Robert C. School of Biological Sciences DRNTU::Science::Biological sciences::Biochemistry In preparation for mammalian cell division, microtubules repeatedly probe the cytoplasm to capture chromosomes and assemble the mitotic spindle. Critical features of this microtubule system are the formation of radial arrays centered at the centrosomes and dynamic instability, leading to persistent cycles of polymerization and depolymerization. Here, we show that actin homolog, ParM-R1 that drives segregation of the R1 multidrug resistance plasmid from Escherichia coli, can also self-organize in vitro into asters, which resemble astral microtubules. ParM-R1 asters grow from centrosome-like structures consisting of interconnected nodes related by a pseudo 8-fold symmetry. In addition, we show that ParM-R1 is able to perform persistent microtubule-like oscillations of assembly and disassembly. In vitro, a whole population of ParM-R1 filaments is synchronized between phases of growth and shrinkage, leading to prolonged synchronous oscillations even at physiological ParM-R1 concentrations. These results imply that the selection pressure to reliably segregate DNA during cell division has led to common mechanisms within diverse segregation machineries. 2013-10-30T02:14:07Z 2019-12-06T21:42:00Z 2013-10-30T02:14:07Z 2019-12-06T21:42:00Z 2012 2012 Journal Article Popp, D., Narita, A., Lee, L. J., Larsson, M., & Robinson, R. C. (2012). Microtubule-like properties of the bacterial actin homolog ParM-R1. Journal of biological chemistry, 287(44), 37078-37088. https://hdl.handle.net/10356/104888 http://hdl.handle.net/10220/17016 10.1074/jbc.M111.319491 22908230 en Journal of biological chemistry |
| spellingShingle | DRNTU::Science::Biological sciences::Biochemistry Popp, David. Narita, Akihiro. Lee, Lin Jie. Larsson, Mårten. Robinson, Robert C. Microtubule-like properties of the bacterial actin homolog ParM-R1 |
| title | Microtubule-like properties of the bacterial actin homolog ParM-R1 |
| title_full | Microtubule-like properties of the bacterial actin homolog ParM-R1 |
| title_fullStr | Microtubule-like properties of the bacterial actin homolog ParM-R1 |
| title_full_unstemmed | Microtubule-like properties of the bacterial actin homolog ParM-R1 |
| title_short | Microtubule-like properties of the bacterial actin homolog ParM-R1 |
| title_sort | microtubule like properties of the bacterial actin homolog parm r1 |
| topic | DRNTU::Science::Biological sciences::Biochemistry |
| url | https://hdl.handle.net/10356/104888 http://hdl.handle.net/10220/17016 |
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