TSpred : a web server for the rational design of temperature-sensitive mutants
Temperature sensitive (Ts) mutants of proteins provide experimentalists with a powerful and reversible way of conditionally expressing genes. The technique has been widely used in determining the role of gene and gene products in several cellular processes. Traditionally, Ts mutants are generated by...
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2014
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/104966 http://hdl.handle.net/10220/20390 |
| _version_ | 1811687282089918464 |
|---|---|
| author | Tan, Kuan Pern Khare, Shruti Varadarajan, Raghavan Madhusudhan, Mallur Srivatsan |
| author2 | School of Computer Engineering |
| author_facet | School of Computer Engineering Tan, Kuan Pern Khare, Shruti Varadarajan, Raghavan Madhusudhan, Mallur Srivatsan |
| author_sort | Tan, Kuan Pern |
| collection | NTU |
| description | Temperature sensitive (Ts) mutants of proteins provide experimentalists with a powerful and reversible way of conditionally expressing genes. The technique has been widely used in determining the role of gene and gene products in several cellular processes. Traditionally, Ts mutants are generated by random mutagenesis and then selected though laborious large-scale screening. Our web server, TSpred (http://mspc.bii.a-star.edu.sg/TSpred/), now enables users to rationally design Ts mutants for their proteins of interest. TSpred uses hydrophobicity and hydrophobic moment, deduced from primary sequence and residue depth, inferred from 3D structures to predict/identify buried hydrophobic residues. Mutating these residues leads to the creation of Ts mutants. Our method has been experimentally validated in 36 positions in six different proteins. It is an attractive proposition for Ts mutant engineering as it proposes a small number of mutations and with high precision. The accompanying web server is simple and intuitive to use and can handle proteins and protein complexes of different sizes. |
| first_indexed | 2024-10-01T05:13:50Z |
| format | Journal Article |
| id | ntu-10356/104966 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:13:50Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/1049662022-02-16T16:26:29Z TSpred : a web server for the rational design of temperature-sensitive mutants Tan, Kuan Pern Khare, Shruti Varadarajan, Raghavan Madhusudhan, Mallur Srivatsan School of Computer Engineering DRNTU::Engineering::Computer science and engineering Temperature sensitive (Ts) mutants of proteins provide experimentalists with a powerful and reversible way of conditionally expressing genes. The technique has been widely used in determining the role of gene and gene products in several cellular processes. Traditionally, Ts mutants are generated by random mutagenesis and then selected though laborious large-scale screening. Our web server, TSpred (http://mspc.bii.a-star.edu.sg/TSpred/), now enables users to rationally design Ts mutants for their proteins of interest. TSpred uses hydrophobicity and hydrophobic moment, deduced from primary sequence and residue depth, inferred from 3D structures to predict/identify buried hydrophobic residues. Mutating these residues leads to the creation of Ts mutants. Our method has been experimentally validated in 36 positions in six different proteins. It is an attractive proposition for Ts mutant engineering as it proposes a small number of mutations and with high precision. The accompanying web server is simple and intuitive to use and can handle proteins and protein complexes of different sizes. Published version 2014-08-25T02:12:36Z 2019-12-06T21:43:41Z 2014-08-25T02:12:36Z 2019-12-06T21:43:41Z 2014 2014 Journal Article Tan, K. P., Khare, S., Varadarajan, R., & Madhusudhan, M. S. (2014). TSpred: a web server for the rational design of temperature-sensitive mutants. Nucleic Acids Research, 42(W1), W277-W284. 0305-1048 https://hdl.handle.net/10356/104966 http://hdl.handle.net/10220/20390 10.1093/nar/gku319 24782523 en Nucleic acids research © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. application/pdf |
| spellingShingle | DRNTU::Engineering::Computer science and engineering Tan, Kuan Pern Khare, Shruti Varadarajan, Raghavan Madhusudhan, Mallur Srivatsan TSpred : a web server for the rational design of temperature-sensitive mutants |
| title | TSpred : a web server for the rational design of temperature-sensitive mutants |
| title_full | TSpred : a web server for the rational design of temperature-sensitive mutants |
| title_fullStr | TSpred : a web server for the rational design of temperature-sensitive mutants |
| title_full_unstemmed | TSpred : a web server for the rational design of temperature-sensitive mutants |
| title_short | TSpred : a web server for the rational design of temperature-sensitive mutants |
| title_sort | tspred a web server for the rational design of temperature sensitive mutants |
| topic | DRNTU::Engineering::Computer science and engineering |
| url | https://hdl.handle.net/10356/104966 http://hdl.handle.net/10220/20390 |
| work_keys_str_mv | AT tankuanpern tspredawebserverfortherationaldesignoftemperaturesensitivemutants AT khareshruti tspredawebserverfortherationaldesignoftemperaturesensitivemutants AT varadarajanraghavan tspredawebserverfortherationaldesignoftemperaturesensitivemutants AT madhusudhanmallursrivatsan tspredawebserverfortherationaldesignoftemperaturesensitivemutants |