Automated assessment of β-cell area and density per islet and patient using TMEM27 and BACE2 immunofluorescence staining in human pancreatic β-cells
In this study we aimed to establish an unbiased automatic quantification pipeline to assess islet specific features such as β-cell area and density per islet based on immunofluorescence stainings. To determine these parameters, the in vivo protein expression levels of TMEM27 and BACE2 in pancreatic...
Main Authors: | Rechsteiner, Markus P., Floros, Xenofon, Boehm, Bernhard O., Marselli, Lorella, Marchetti, Piero, Stoffel, Markus, Moch, Holger, Spinas, Giatgen A. |
---|---|
Other Authors: | Pietropaolo, Massimo |
Format: | Journal Article |
Language: | English |
Published: |
2014
|
Subjects: | |
Online Access: | https://hdl.handle.net/10356/105082 http://hdl.handle.net/10220/20420 |
Similar Items
-
Apolipoprotein CIII links islet insulin resistance to β-cell failure in diabetes
by: Leibiger, Ingo B., et al.
Published: (2015) -
Lack of ZnT8 protects pancreatic islets from hypoxia- and cytokine-induced cell death
by: Karsai, Maria, et al.
Published: (2022) -
Single-cell transcriptomics of East-Asian pancreatic islets cells
by: Tay, Vanessa Shi Yun, et al.
Published: (2018) -
Proinflammatory cytokines suppress nonsense-mediated RNA decay to impair regulated transcript isoform processing in pancreatic β cells
by: Ghiasi, Seyed M., et al.
Published: (2024) -
Tmem119-EGFP and Tmem119-CreERT2 Transgenic Mice for Labeling and Manipulating Microglia
by: Kaiser, Tobias, et al.
Published: (2020)