Designed di-heme binding helical transmembrane protein
De novo designing of functional membrane proteins is fundamental in terms of understanding the structure, folding, and stability of membrane proteins. In this work, we report the design and characterization of a transmembrane protein, termed HETPRO (HEme-binding Transmembrane PROtein), that binds tw...
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2014
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| Online Access: | https://hdl.handle.net/10356/105143 http://hdl.handle.net/10220/20460 http://dx.doi.org/10.1002/cbic.201402142 |
| _version_ | 1824454316038029312 |
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| author | Mahajan, Mukesh Bhattacharjya, Surajit |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Mahajan, Mukesh Bhattacharjya, Surajit |
| author_sort | Mahajan, Mukesh |
| collection | NTU |
| description | De novo designing of functional membrane proteins is fundamental in terms of understanding the structure, folding, and stability of membrane proteins. In this work, we report the design and characterization of a transmembrane protein, termed HETPRO (HEme-binding Transmembrane PROtein), that binds two molecules of heme in a membrane and catalyzes oxidation/reduction reactions. The primary structure of HETPRO has been optimized in a guided fashion, from an antimicrobial peptide, for transmembrane orientation, defined 3D structure, and functions. HETPRO assembles into a tetrameric form, from an apo dimeric helical structure, in complex with cofactor in detergent micelles. The NMR structure of the apo HETPRO in micelles reveals an antiparallel helical dimer that inserts into the nonpolar core of detergent micelles. The well-defined structure of HETPRO and its ability to bind to heme moieties could be utilized to develop a functional membrane protein mimic for electron transport and photosystems. |
| first_indexed | 2025-02-19T03:20:22Z |
| format | Journal Article |
| id | ntu-10356/105143 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:20:22Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/1051432019-12-06T21:46:29Z Designed di-heme binding helical transmembrane protein Mahajan, Mukesh Bhattacharjya, Surajit School of Biological Sciences DRNTU::Science::Biological sciences De novo designing of functional membrane proteins is fundamental in terms of understanding the structure, folding, and stability of membrane proteins. In this work, we report the design and characterization of a transmembrane protein, termed HETPRO (HEme-binding Transmembrane PROtein), that binds two molecules of heme in a membrane and catalyzes oxidation/reduction reactions. The primary structure of HETPRO has been optimized in a guided fashion, from an antimicrobial peptide, for transmembrane orientation, defined 3D structure, and functions. HETPRO assembles into a tetrameric form, from an apo dimeric helical structure, in complex with cofactor in detergent micelles. The NMR structure of the apo HETPRO in micelles reveals an antiparallel helical dimer that inserts into the nonpolar core of detergent micelles. The well-defined structure of HETPRO and its ability to bind to heme moieties could be utilized to develop a functional membrane protein mimic for electron transport and photosystems. 2014-09-08T08:05:08Z 2019-12-06T21:46:29Z 2014-09-08T08:05:08Z 2019-12-06T21:46:29Z 2014 2014 Journal Article Mahajan, M., & Bhattacharjya, S. (2014). Designed di-heme binding helical transmembrane protein. ChemBioChem, 15(9), 1257-1262. 1439-4227 https://hdl.handle.net/10356/105143 http://hdl.handle.net/10220/20460 http://dx.doi.org/10.1002/cbic.201402142 en ChemBioChem © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| spellingShingle | DRNTU::Science::Biological sciences Mahajan, Mukesh Bhattacharjya, Surajit Designed di-heme binding helical transmembrane protein |
| title | Designed di-heme binding helical transmembrane protein |
| title_full | Designed di-heme binding helical transmembrane protein |
| title_fullStr | Designed di-heme binding helical transmembrane protein |
| title_full_unstemmed | Designed di-heme binding helical transmembrane protein |
| title_short | Designed di-heme binding helical transmembrane protein |
| title_sort | designed di heme binding helical transmembrane protein |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/105143 http://hdl.handle.net/10220/20460 http://dx.doi.org/10.1002/cbic.201402142 |
| work_keys_str_mv | AT mahajanmukesh designeddihemebindinghelicaltransmembraneprotein AT bhattacharjyasurajit designeddihemebindinghelicaltransmembraneprotein |