Nano-imaging collagen by atomic force, near-field and nonlinear microscope
As the most abundant protein in the human body, collagen has a very important role in vast numbers of bio-medical applications. The unique second order nonlinear properties of fibrillar collagen make it a very important index in nonlinear optical imaging based disease diagnosis of the brain, skin, l...
| Main Authors: | , , , , , , |
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| Format: | Conference Paper |
| Language: | English |
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2015
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| Online Access: | https://hdl.handle.net/10356/105263 http://hdl.handle.net/10220/26037 http://dx.doi.org/10.1117/12.2078911 |
| _version_ | 1811694396924493824 |
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| author | Lim, Ken Choong Tang, Jinkai Li, Hao Ng, Boon Ping Kok, Shaw Wei Wang, Qijie Zhang, Ying |
| author2 | Cartwright, Alexander N. |
| author_facet | Cartwright, Alexander N. Lim, Ken Choong Tang, Jinkai Li, Hao Ng, Boon Ping Kok, Shaw Wei Wang, Qijie Zhang, Ying |
| author_sort | Lim, Ken Choong |
| collection | NTU |
| description | As the most abundant protein in the human body, collagen has a very important role in vast numbers of bio-medical applications. The unique second order nonlinear properties of fibrillar collagen make it a very important index in nonlinear optical imaging based disease diagnosis of the brain, skin, liver, colon, kidney, bone, heart and other organs in the human body. The second-order nonlinear susceptibility of collagen has been explored at the macroscopic level and was explained as a volume-averaged molecular hyperpolarizability. However, details about the origin of optical second harmonic signals from collagen fibrils at the molecular level are still not clear. Such information is necessary for accurate interpolation of bio-information from nonlinear optical imaging techniques. The later has shown great potential in collagen based disease diagnosis methodologies. In this paper, we report our work using an atomic force microscope (AFM), near field (SNOM) and nonlinear laser scanning microscope (NLSM) to study the structure of collagen fibrils and other pro-collagen structures. |
| first_indexed | 2024-10-01T07:06:55Z |
| format | Conference Paper |
| id | ntu-10356/105263 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:06:55Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/1052632019-12-06T21:48:18Z Nano-imaging collagen by atomic force, near-field and nonlinear microscope Lim, Ken Choong Tang, Jinkai Li, Hao Ng, Boon Ping Kok, Shaw Wei Wang, Qijie Zhang, Ying Cartwright, Alexander N. Nicolau, Dan V. School of Electrical and Electronic Engineering Progress in biomedical optics and imaging - Proceedings of SPIE DRNTU::Engineering::Electrical and electronic engineering::Optics, optoelectronics, photonics As the most abundant protein in the human body, collagen has a very important role in vast numbers of bio-medical applications. The unique second order nonlinear properties of fibrillar collagen make it a very important index in nonlinear optical imaging based disease diagnosis of the brain, skin, liver, colon, kidney, bone, heart and other organs in the human body. The second-order nonlinear susceptibility of collagen has been explored at the macroscopic level and was explained as a volume-averaged molecular hyperpolarizability. However, details about the origin of optical second harmonic signals from collagen fibrils at the molecular level are still not clear. Such information is necessary for accurate interpolation of bio-information from nonlinear optical imaging techniques. The later has shown great potential in collagen based disease diagnosis methodologies. In this paper, we report our work using an atomic force microscope (AFM), near field (SNOM) and nonlinear laser scanning microscope (NLSM) to study the structure of collagen fibrils and other pro-collagen structures. Published version 2015-06-23T07:56:15Z 2019-12-06T21:48:18Z 2015-06-23T07:56:15Z 2019-12-06T21:48:18Z 2015 2015 Conference Paper Lim, K. C., Tang, J., Li, H., Ng, B. P., Kok, S. W., Wang, Q., et al. (2015). Nano-imaging collagen by atomic force, near-field and nonlinear microscope. Progress in biomedical optics and imaging - Proceedings of SPIE, 9337, 933709-. https://hdl.handle.net/10356/105263 http://hdl.handle.net/10220/26037 http://dx.doi.org/10.1117/12.2078911 en © 2015 Society of Photo-Optical Instrumentation Engineers (SPIE). This paper was published in Progress in Biomedical Optics and Imaging - Proceedings of SPIE and is made available as an electronic reprint (preprint) with permission of Society of Photo-Optical Instrumentation Engineers (SPIE). The paper can be found at the following official DOI: [http://dx.doi.org/10.1117/12.2078911]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. application/pdf |
| spellingShingle | DRNTU::Engineering::Electrical and electronic engineering::Optics, optoelectronics, photonics Lim, Ken Choong Tang, Jinkai Li, Hao Ng, Boon Ping Kok, Shaw Wei Wang, Qijie Zhang, Ying Nano-imaging collagen by atomic force, near-field and nonlinear microscope |
| title | Nano-imaging collagen by atomic force, near-field and nonlinear microscope |
| title_full | Nano-imaging collagen by atomic force, near-field and nonlinear microscope |
| title_fullStr | Nano-imaging collagen by atomic force, near-field and nonlinear microscope |
| title_full_unstemmed | Nano-imaging collagen by atomic force, near-field and nonlinear microscope |
| title_short | Nano-imaging collagen by atomic force, near-field and nonlinear microscope |
| title_sort | nano imaging collagen by atomic force near field and nonlinear microscope |
| topic | DRNTU::Engineering::Electrical and electronic engineering::Optics, optoelectronics, photonics |
| url | https://hdl.handle.net/10356/105263 http://hdl.handle.net/10220/26037 http://dx.doi.org/10.1117/12.2078911 |
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